Proteomics analysis of the developing fish oocyte

Chapovetsky, Victoria; Gattegno, Tamar; Admon, Arie

doi:10.1007/978-1-4020-6235-3_4

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…These roles cannot be followed by genomic or transcriptomic technologies, necessitating application of proteomics for their elucidation. While proteomic profiling has been widely employed to study the cell biology of oocytes in many species, including humans, mice, pigs, fish and insects [ 16 ], it has rarely been applied to studies of fish egg quality. The spectrum of proteins present in eggs was partly evaluated and a few proteins associated with egg quality were identified in studies of rainbow trout ( Oncorhynchus mykiss ) [ 17 ], European sea bass ( Dicentrarchus labrax ) [ 18 ], Eurasian perch ( Perca fluviatilis ) [ 19 ], and hapuku ( Polyprion oxygeneios ) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

et al. 2017

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Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry. Obtained spectra were searched against a zebrafish proteome database and detected proteins were annotated, categorized and quantified based on normalized spectral counts. Manually curated and automated enrichment analyses revealed poor quality eggs to be deficient of proteins involved in protein synthesis and energy and lipid metabolism, and of some vitellogenin products and lectins, and to have a surfeit of proteins involved in endo-lysosomal activities, autophagy, and apoptosis, and of some oncogene products, lectins and egg envelope proteins. Results of pathway and network analyses suggest that this aberrant proteomic profile results from failure of oocytes giving rise to poor quality eggs to properly transit through final maturation, and implicated Wnt signaling in the etiology of this defect. Quantitative comparisons of abundant proteins in good versus poor quality eggs revealed 17 candidate egg quality markers. Thus, the zebrafish egg proteome is clearly linked to embryo developmental potential, a phenomenon that begs further investigation to elucidate the root causes of poor egg quality, presently a serious and intractable problem in livestock and human reproductive medicine.

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Section: Introductionmentioning

confidence: 99%

Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

et al. 2017

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“…Proteomic profiling has long been employed to study the cell biology of oocytes in many species, including humans, mice, pigs, fish and insects [ 17 ], as well as in investigations of egg quality in fishes such as rainbow trout ( Oncorhynchus mykiss ) [ 18 ], European sea bass ( Dicentrarchus labrax ) [ 19 ], Eurasian perch ( Perca fluviatilis ) [ 20 ], and hapuku ( Polyprion oxygeneios ) [ 21 ]. A comparison of the global proteomes of different quality eggs from zebrafish revealed a number of proteins as potential quality markers and, more importantly, several molecular mechanisms and related physiological processes associated with egg quality in this species [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteome profiling reveals molecular hallmarks of egg quality in Atlantic halibut: impairments of transcription and protein folding impede protein and energy homeostasis during early development

et al. 2022

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Background Tandem mass tag spectrometry (TMT labeling-LC-MS/MS) was utilized to examine the global proteomes of Atlantic halibut eggs at the 1-cell-stage post fertilization. Comparisons were made between eggs judged to be of good quality (GQ) versus poor quality (BQ) as evidenced by their subsequent rates of survival for 12 days. Altered abundance of selected proteins in BQ eggs was confirmed by parallel reaction monitoring spectrometry (PRM-LC-MS/MS). Correspondence of protein levels to expression of related gene transcripts was examined via qPCR. Potential mitochondrial differences between GQ and BQ eggs were assessed by transmission electron microscopy (TEM) and measurements of mitochondrial DNA (mtDNA) levels. Results A total of 115 proteins were found to be differentially abundant between GQ and BQ eggs. Frequency distributions of these proteins indicated higher protein folding activity in GQ eggs compared to higher transcription and protein degradation activities in BQ eggs. BQ eggs were also significantly enriched with proteins related to mitochondrial structure and biogenesis. Quantitative differences in abundance of several proteins with parallel differences in their transcript levels were confirmed in egg samples obtained over three consecutive reproductive seasons. The observed disparities in global proteome profiles suggest impairment of protein and energy homeostasis related to unfolded protein response and mitochondrial stress in BQ eggs. TEM revealed BQ eggs to contain significantly higher numbers of mitochondria, but differences in corresponding genomic mtDNA (mt-nd5 and mt-atp6) levels were not significant. Mitochondria from BQ eggs were significantly smaller with a more irregular shape and a higher number of cristae than those from GQ eggs. Conclusion The results of this study indicate that BQ Atlantic halibut eggs are impaired at both transcription and translation levels leading to endoplasmic reticulum and mitochondrial disorders. Observation of these irregularities over three consecutive reproductive seasons in BQ eggs from females of diverse background, age and reproductive experience indicates that they are a hallmark of poor egg quality. Additional research is needed to discover when in oogenesis and under what circumstances these defects may arise. The prevalence of this suite of markers in BQ eggs of diverse vertebrate species also begs investigation.

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“…Proteomic profiling has been widely employed to study the cell biology of oocytes in many species, including humans, mice, pigs, fish and insects (17) (Chapovetsky et al, 2007), but it has been less than two decades since it has been considered as a useful and practical tool to study fish egg quality, i.e. rainbow trout (Oncorhynchus mykiss) (18) (Rime et al, 2004), European sea bass (Dicentrarchus labrax) (19) (Crespel et al, 2008), Eurasian perch (Perca fluviatilis) (20) (Castets et al, 2012), and hapuku (Polyprion oxygeneios) (21) (Kohn et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms involved in Atlantic halibut (Hippoglossus hippoglossus) egg quality: impairments at transcription and protein folding levels induce inefficient protein and energy homeostasis during early development

Yilmaz

Jensen

Harboe

et al. 2022

Preprint

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Background: Reproductive success and normal development in all animals are dependent on egg quality and developmental competence of the produced embryo. This study employed tandem mass tags labeling based liquid chromatography tandem mass spectrometry for egg proteomic profiling to investigate differences in the global proteome of good versus poor quality Atlantic halibut eggs at 1-cell stage post fertilization. Results: A total of 115 proteins were found to be differentially abundant between good and poor quality eggs. Frequency distribution of these proteins revealed higher protein folding activity in good quality eggs in comparison to higher transcription and protein degradation activities in poor quality eggs (p < 0.05). Poor quality halibut eggs were significantly enriched with additional proteins related to mitochondrial structure and biogenesis (p < 0.05). The differential abundance of a selection of proteins was first confirmed at gene expression level using a transcriptomic approach followed by a targeted proteomic approach (parallel reaction monitoring based mass spectrometry) in biological samples obtained from two consecutive reproductive seasons. The findings of global proteome profiling, together with the validation of differential abundance of targeted proteins and their related genes, suggest impairments in protein and energy homeostasis which might be related to unfolded protein response and mitochondrial stress in poor quality eggs. Additional transmission electron microscopy studies were taken to assess potential differences in abundance and morphological integrity of mitochondria between good and poor quality eggs. Observations reveal poor quality eggs to contain significantly higher number of mitochondria with higher number of cristae. These mitochondria, however, are significantly smaller and have a more irregular shape than those found in high-quality eggs. Therewithal difference in mtDNA levels represented by mt-nd5 and mt-atp6 genomic DNA abundance in this study, were found to be not statistically significant (p > 0.05) between good and bad quality eggs at both 1 hpf and 24 hpf stages. Conclusion: Overall evidence from this study indicate that poor quality eggs undergo impairments at both transcription and translation level leading to endoplasmic reticulum and mitochondrial deficiencies. Additional research may be required to expediate the details and the potential of these impairments occurring in different species. Nonetheless, this study will pave the way for future research and will help in acceleration of recent advances in the field of embryonic developmental competence of living organisms.

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Proteomics analysis of the developing fish oocyte

Cited by 6 publications

References 41 publications

Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

Quantitative proteome profiling reveals molecular hallmarks of egg quality in Atlantic halibut: impairments of transcription and protein folding impede protein and energy homeostasis during early development

Molecular mechanisms involved in Atlantic halibut (Hippoglossus hippoglossus) egg quality: impairments at transcription and protein folding levels induce inefficient protein and energy homeostasis during early development

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