Proteomics analysis of the germinating seeds of Cariniana legalis (Mart.) Kuntze (Meliaceae): an endangered species of the Brazilian Atlantic Rainforest

Trindade, Bianca Machado Campos; Reis, Robert L.; Vale, Ellen Moura; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1007/s40415-017-0419-7

Cited by 2 publications

(4 citation statements)

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“…Protein extraction -Soluble proteins were extracted according to the method described by Trindade et al (2017). Protein extracts were prepared from three biological samples (100 mg FM each sample) obtained at each time of analysis (0, 3, 6, and 12 months of storage).…”

Section: Methodsmentioning

confidence: 99%

“…Three 2-DE gels were performed for each biological sample using aliquots containing 700 μg of protein according to the method described by Trindade et al (2017). The samples were precipitated overnight in five volumes of 0.1 M ammonium acetate (Vetec, Rio de Janeiro, Brazil) in methanol (Merck, Darmstadt, Germany) at − 20 °C.…”

Section: Two-dimensional Electrophoresis (2-de) Analysis -mentioning

confidence: 99%

“…analysis -A NanoAcquity ultra-performance liquid chromatography (UPLC) column connected to a SYNAPT G2-Si high-definition mass spectrometry (HDMS) system (Waters, Manchester, UK) for electrospray ionization-liquid chromatography-tandem mass spectrometry (ESI-LC-MS/MS) using a BEH 130 C18 1.7 μm (100 μm × 100 mm) column at a flow rate of 500 nL min −1 was used for mass spectrometric analysis according to Trindade et al (2017). For peptide elution, a binary gradient was used, with mobile phase A consisting of water (Tedia, Fairfield, OH, USA) and 0.1% FA and mobile phase B consisting of acetonitrile and 0.1% FA.…”

Section: Protein Identification By Ms/ms and Bioinformaticsmentioning

confidence: 99%

“…The results suggested that the presence of glucose and fructose, a higher level of the polyamine putrescine, and lower levels of amino acids may be associated with greater reductions in vigor and seedling emergence in this species (Sousa et al 2016). In addition, a recent study analyzed the proteomic profile of C. legalis seeds during germination using two-dimensional gel electrophoresis (2-DE) and identified several proteins that play an important role in the germination process (Trindade et al 2017). These results have improved our understanding of the molecular processes involved in seed germination of C. legalis.…”

Section: Introductionmentioning

confidence: 99%

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Storage time affects the germination and proteomic profile of seeds of Cariniana legalis (Mart.) O. Kuntze (Lecythidaceae), an endangered tree species native to the Brazilian Atlantic Forest

et al. 2019

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Seed aging is a critical process during storage and plays an important role in maintaining seed vigor. The present study aimed to investigate the effects of seed storage on germination and the differential abundance of proteins associated with viability loss in Cariniana legalis (Mart.) O. Kuntze. Seeds were stored for 12 months at 6 °C in plastic bags. Analyses of germination were performed at time 0 (newly harvested seeds) and after 3, 6, and 12 months of storage. To identify differentially abundant proteins, two-dimensional electrophoresis was performed, followed by tandem mass spectrometric (MS/ MS) analysis. Germination, the germination speed index and the moisture content were significantly reduced during storage. Proteomic analysis revealed 50 differentially abundant proteins among the times analyzed; these proteins were classified into several functional classes related to the biological, metabolic, cellular, oxidation-reduction, carbohydrate metabolism, storage, aging, cell wall organization or biogenesis, response to stress and stimulus, and proteolysis categories. The correlation between seed aging and proteome changes suggests that specific proteins may be involved in the mechanisms underlying seed deterioration, including ferritin, seed maturation-like, 2,4-dienoyl-CoA reductase, 11S globulin, polygalacturonases, superoxide dismutase, heat shock proteins, and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Our data showed that increasing storage time significantly reduced germination and revealed the regulation of differentially abundant proteins, which may be related to the reduction in seed viability in C. legalis.

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Section: Methodsmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis (2-de) Analysis -mentioning

confidence: 99%

Section: Protein Identification By Ms/ms and Bioinformaticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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