Proteomics analysis to understand the ABA stimulation of wound suberization in kiwifruit

Han, Xueyuan; Lu, Wenjing; Wei, Xiaopeng; Li, Li; Mao, Linchun; Zhao, Yuying

doi:10.1016/j.jprot.2017.11.018

Cited by 55 publications

(26 citation statements)

References 56 publications

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“…The phenylpropanoid pathway produces phenolic compounds that contribute to fruit pigmentation and the disease resistance response during the ripening process of fruits [73]. Han et al [74] reported increased transcript levels of the KCS gene, a gene that involves very long-chain fatty acid biosynthesis of lipid pathway following the application of abscisic acid (ABA) for wound suberization. Furthermore, there was an upregulation of the gene that encodes β-carotene hydroxylase, an enzyme in the zeaxanthin biosynthetic pathway which could help for protection against stress [75].…”

Section: Stress-related Genes Due To Ethylene Treatmentmentioning

confidence: 99%

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

et al. 2020

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Fruit ripening involves changes in physical, physiological and metabolic activities through the actions of enzymes and regulatory genes. This study was initiated to identify the genes related to the ripening of kiwifruit. Gold ‘Haegeum’ kiwifruit is a yellow-fleshed kiwifruit cultivar usually used for fresh marketing. The fruit is harvested at a physiologically mature but unripe stage for proper storage, marketing distribution and longer shelf life. To identify the differentially expressed genes (DEGs) during ripening, fruit treated with ethylene were compared with control fruit that ripened naturally without ethylene treatment. Firmness, respiration rate, ethylene production rate, total soluble solids (TSS), titratable acidity (TA), brix acid ratio (BAR) and overall acceptability were taken during the study as fruit ripening indicators. Total mRNAs were sequenced by Illumina high-throughput sequencing platform and the transcriptome gene set was constructed by de novo assembly. We identified 99,601 unigenes with an average length of 511.77 bp in transcriptome contigs. A total of 28,582 differentially expressed unigenes were identified in the ethylene treatment vs. control. Of these 28,582 unigenes, 13,361 and 15,221 genes were up- and downregulated, respectively, in the treated fruit. The results also showed that 1682 and 855 genes were up- and downregulated, respectively, more than 2-fold at p < 0.05 in fruit treated with ethylene as compared with the control fruit. Moreover, we identified 75 genes showing significantly different expression; 42 were upregulated, and 33 were downregulated. A possible category of the identified ripening-related genes was also made. The findings of this study will add to the available information on the effect of ethylene treatment on ripening and the related changes of kiwifruit at the genomic level, and it could assist the further study of genes related to ripening for kiwifruit breeding and improvement.

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Section: Stress-related Genes Due To Ethylene Treatmentmentioning

confidence: 99%

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

et al. 2020

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“…The w-functionalized suberin monomers, w-hydroxyacids and a, w-diacids, play a critical role in suberin assemble. The a, wdiacids are esterified with glycerol to form the glycerol-a, wdiacid-glycerol unit, the basic three-dimensional structure of suberin (Graç a and Pereira, 2000;Graç a and Pereira, 2015;Jin et al, 2018). Moreover, w-hydroxyacids act as substrates for feruloyl transferase and glycerol 3-phosphate acyltransferase esterifying with ferulic acid and glycerol, respectively, to crosslink aliphatics and aromatics (Beisson et al, 2007;Gou et al, 2009;Molina et al, 2009;Serra et al, 2010;Yang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

Wei

Mao

et al. 2020

Front. Plant Sci.

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Wound attack stimulates accumulation of abscisic acid (ABA) that activates a number of genes associated with wound suberization of plants. Cytochrome P450 fatty acid whydroxylase CYP86A1 catalyzes w-hydroxylation of fatty acids to form the wfunctionalized monomers that play a pivotal role in suberin synthesis. However, the transcriptional regulation of ABA signaling on AchnCYP86A1 has not been characterized in kiwifruit. In this study, AchnCYP86A1, a kiwifruit homolog of Arabidopsis AtCYP86A1, was isolated. AchnCYP86A1-overexpressed N. benthamiana leaves displayed that the AchnCYP86A1 functioned as a fatty acid w-hydroxylase associated with synthesis of suberin monomer. The regulatory function of three transcription factors (TFs, including AchnMYC2, AchnMYB41 and AchnMYB107) on AchnCYP86A1 was identified. All the three TFs were localized in nucleus and could individually interact with AchnCYP86A1 promoter to activate gene expression in yeast one-hybrid and dual-luciferase assays. The findings were further demonstrated in transient overexpressed N. benthamiana, in which all TFs notably elevated the expression of aliphatic synthesis genes including CYP86A1 and the accumulation of w-hydroxyacids, a, w-diacids, fatty acids and primary alcohols. Moreover, exogenous ABA induced the expression of AchnMYC2, AchnMYB41 and AchnMYB107 that promoted AchnCYP86A1 involving in suberin monomer formation. Contrary to the inductive effects of ABA, however, fluridone (an inhibitor of ABA biosynthesis) inhibited the three TFs expression and suberin monomer formation. These results indicate that AchnMYC2, AchnMYB41 and AchnMYB107 positively regulate suberin monomer synthesis by activating AchnCYP86A1 promoter in response to ABA.

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“…ABA is especially important for plant adaptation to abiotic stress. In particular, the ability of ABA to stimulate suberin deposition during wounding and a deficiency in mineral nutrition has been identified [13,14]. ABA has been shown to increase the expression of LTP genes.…”

Section: Introductionmentioning

confidence: 99%

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

et al. 2019

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Lipid transfer proteins (LTPs) are a class of small, cationic proteins that bind and transfer lipids and play an important role in plant defense. However, their precise biological role in plants under adverse conditions including salinity and possible regulation by stress hormone abscisic acid (ABA) remains unknown. In this work, we studied the localization of LTPs and ABA in the roots of pea plants using specific antibodies. Presence of LTPs was detected on the periphery of the cells mainly located in the phloem. Mild salt stress (50 mM NaCI) led to slowing plant growth and higher immunostaining for LTPs in the phloem. The deposition of suberin in Casparian bands located in the endoderma revealed with Sudan III was shown to be more intensive under salt stress and coincided with the increased LTP staining. All obtained data suggest possible functions of LTPs in pea roots. We assume that these proteins can participate in stress-induced pea root suberization or in transport of phloem lipid molecules. Salt stress increased ABA immunostaining in pea root cells but its localization was different from that of the LTPs. Thus, we failed to confirm the hypothesis regarding the direct influence of ABA on the level of LTPs in the salt-stressed root cells.

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Proteomics analysis to understand the ABA stimulation of wound suberization in kiwifruit

Cited by 55 publications

References 56 publications

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

Transcriptome Analysis of ‘Haegeum’ Gold Kiwifruit Following Ethylene Treatment to Improve Postharvest Ripening Quality

Three Transcription Activators of ABA Signaling Positively Regulate Suberin Monomer Synthesis by Activating Cytochrome P450 CYP86A1 in Kiwifruit

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

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