Proteomics and metabolomics: The molecular make‐up of toxic aromatic pollutant bioremediation

Abstract

Microbial-mediated attenuation of toxic aromatic pollutants offers great potential for the restoration of contaminated environments in an ecologically acceptable manner. However, incomplete biological information regarding the regulation of growth and metabolism in many microbial communities restricts progress in the site-specific mineralization process. In the postgenomic era, recent advances in MS have allowed enormous progress in proteomics and elucidated many complex biological interactions. These research forefronts are now expanding toward the analysis of low-molecular-weight primary and secondary metabolites analysis, i.e., metabolomics. The advent of 2-DE in conjunction with MS offers a promising approach to address the molecular mechanisms of bioremediation. The two fields of proteomics and metabolomics have thus far worked separately to identify proteins and primary and secondary metabolites during bioremediation. A simultaneous study combining functional proteomics and metabolomics, i.e., proteometabolomics would create a system-wide approach to studying site-specific microorganisms during active mineralization processes. This article deals with advances in environmental proteomics and metabolomics and advocates the simultaneous study of both technologies to implement cell-free bioremediation.

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

“…[62][63][64][65][66][67] A aplicação da metabolômica na área ambiental é muito ampla e envolve o estudo de organismos aquáticos, [67][68][69][70] de plantas, 71 e como estes respondem ao estresse oxidativo, 72 estresse abiótico, [73][74][75] fatores bióticos, 76 ou fatores bióticos e abióticos simultaneamente. 77 Também são encontrados trabalhos de exposição de organismos a poluentes, 78 18,86 entre outros. Estudos apontam a presença de fármacos em diferentes concentrações em águas de rios, em estações de tratamento de águas, de estuários, entre outros.…”

Molecular Analyses of Microbial Activities Involved in Bioremediation

“…The lack of complete knowledge restricts progress in the site-specifi c mineralization process. In the postgenomic era the emphasis is on 2D PAGE and MS to obtain the incomplete biological information regarding the regulation of growth and metabolism in microbial communities (Singh 2006 ) . Moreover, Singh ( 2006 ) in his review emphasizes the need of combining proteomic and metabolomic studies.…”

“…In the postgenomic era the emphasis is on 2D PAGE and MS to obtain the incomplete biological information regarding the regulation of growth and metabolism in microbial communities (Singh 2006 ) . Moreover, Singh ( 2006 ) in his review emphasizes the need of combining proteomic and metabolomic studies. MS can be used in bioremediation studies for identifi cation, purifi cation, characterization and quantifi cation of key enzymes and metabolites involved in biodegradation pathways as it can be used in conjunction with almost all other techniques.…”

Proteomic detection of proteins involved in perchlorate and chlorate metabolism

“…Perchlorate can impair proper functioning of the thyroid glands at concentrations as low as 24 ppb (Wood 2008). It competitively inhibits iodine uptake by the thyroid glands, by blocking the sodiumiodide symporter protein on thyroid follicle cells (Sellers et al 2007;National Research Council 2005). Biological reduction of perchlorate by microorganisms is the most widely used treatment technology for perchlorate contamination.…”