Proteomics approach to analyze protein profiling related with ADME/Tox in rat treated with Scutellariae radix and Coptidis rhizoma as well as their compatibility

Miao, Qing; Zhao, Yuanyuan; Miao, Peipei; Chen, Ning; Yan, Xuehua; Guo, Chang-E; Chen, Hongying; Zhang, Yujie

doi:10.1016/j.jep.2015.07.044

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Protein expression was determined by western blot, which was performed as previously described (Miao et al. 2015 ). Nrf2 expression was measured in the cytoplasmic and nuclear fractions.…”

Section: Methodsmentioning

confidence: 99%

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

Miao

Wang

Sun

et al. 2022

Pharmaceutical Biology

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Context Puerarin (Pue) and tanshinone IIA (Tan IIA) are often used in combination in the treatment of cerebrovascular diseases. Objective To investigate the neuroprotective effect and synergic mechanism of Pue-Tan IIA on the treatment of ischaemic stroke (IS). Materials and methods IS was induced in rats by middle cerebral artery occlusion (MCAO). Rats were intraperitoneally injected with Pue (36 mg/kg), Tan IIA (7.2 mg/kg), or Pue-Tan IIA (36 and 7.2 mg/kg) for five times [30 min before ischaemia, immediately after reperfusion (0 h), 24, 48, and 72 h after reperfusion]. After administration, neurological function assessment and histological changes in the brain were performed. S-100β and NSE levels were measured to determine the severity of brain injury. Oxidative stress parameters and inflammatory mediators were measured. The proteins involved in Nrf2/ARE signalling pathway were determined by qRT-PCR and western blot. Results After administration, the neurological function scores, infarct volume, S-100β, and NSE levels were significantly reduced in MCAO rats, especially with Pue-Tan IIA treatment ( p < 0.05). All treatments increased T-AOC, CAT, SOD, and GSH activities and reduced GSSG activity and MDA, TNF-α, IL-6, ICAM-1, and COX-2 levels in MCAO rats. Pue-Tan IIA significantly increased Nrf2 expression in the nucleus (1.81-fold) and decreased its expression in the cytoplasm (0.60-fold). Pue-Tan IIA significantly increased the expressions of HO-1 (1.87-fold) and NQO1 (1.76-fold) and decreased Keap1 expression (0.39-fold). Discussion and conclusions The combination of Pue and Tan IIA could alleviate ischaemic brain injury by activating Nrf2/ARE signalling pathway, providing an experimental basis for clinical applications.

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“…Protein expression was determined by western blot, which was performed as previously described (Miao et al. 2015 ). Nrf2 expression was measured in the cytoplasmic and nuclear fractions.…”

Section: Methodsmentioning

confidence: 99%

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

Miao

Wang

Sun

et al. 2022

Pharmaceutical Biology

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“…These results indicated that effects of each drug had different emphasis in angiogenesis and reduced energy consumption, anti-oxidation and anti-adhesion, promotion of angiogenesis, promotion of microangiogenesis. Miao et al [73] explored the effects of single herb Radix Scutellariae, Rhizoma Coptidis and their herbal pair in the liver tissue of rats. Total 78 proteins expressed differently were associated with drug metabolism, energy metabolism, signal transduction and cytoskeleton.…”

Section: Miscellaneousmentioning

confidence: 99%

Differential proteomics for studying action mechanisms of traditional Chinese medicines

Yang

Gao

2019

Chin Med

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Differential proteomics, which has been widely used in studying of traditional Chinese medicines (TCMs) during the past 10 years, is a powerful tool to visualize differentially expressed proteins and analyzes their functions. In this paper, the applications of differential proteomics in exploring the action mechanisms of TCMs on various diseases including cancers, cardiovascular diseases, diabetes, liver diseases, kidney disorders and obesity, etc. were reviewed. Furthermore, differential proteomics in studying of TCMs identification, toxicity, processing and compatibility mechanisms were also included. This review will provide information for the further applications of differential proteomics in TCMs studies.

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Assessment of the Intestinal Permeability of Major Phytocomponents Contained in Gandi Capsules Using Ultra-High Performance Liquid Chromatography Coupled with Electrospray Ionization–Quadrupole-Time of Flight Mass Spectrometry

et al. 2018

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Proteomics approach to analyze protein profiling related with ADME/Tox in rat treated with Scutellariae radix and Coptidis rhizoma as well as their compatibility

Cited by 6 publications

References 24 publications

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

Differential proteomics for studying action mechanisms of traditional Chinese medicines

Assessment of the Intestinal Permeability of Major Phytocomponents Contained in Gandi Capsules Using Ultra-High Performance Liquid Chromatography Coupled with Electrospray Ionization–Quadrupole-Time of Flight Mass Spectrometry

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