Proteomics as a tool to monitor plant-microbe endosymbioses in the rhizosphere

Bestel-Corre, Gwénaëlle; Dumas‐Gaudot, Eliane; Gianinazzi, Silvio

doi:10.1007/s00572-003-0280-3

Cited by 45 publications

(18 citation statements)

References 95 publications

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“…In the last decade, proteomics has succeeded in identifying approximately 400 proteins associated with the development and functioning of both mycorrhizal and rhizobial symbioses (Bestel-Corre et al 2004;Rolfe et al 2003;Trevaskis et al 2002). Other previous studies have focused on leguminous seeds (Mooney and Thelen 2004), roots (Bestel-Corre et al 2002;Morris and Djordjevic 2001;Natera et al 2000), nodules (Natera et al 2000), symbiosome membranes of nodules (Panter et al 2000;Saalbach et al 2002;Wienkoop and Saalbach 2003), symbiosome space , as well as bacteroids (Natera et al 2000) and mitochondria in soybean nodules (Hoa et al 2004).…”

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confidence: 99%

Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

Wan¹,

Torres²,

Ganapathy³

et al. 2005

MPMI

124

122

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Infection of soybean root hairs by Bradyrhizobium japonicum is the first of several complex events leading to nodulation. In the current proteomic study, soybean root hairs after inoculation with B. japonicum were separated from roots. Total proteins were analyzed by two-dimensional (2-D) polyacrylamide gel electrophoresis. In one experiment, 96 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to compare protein profiles between uninoculated roots and root hairs. Another 37 spots, derived from inoculated root hairs over different timepoints, were also analyzed by tandem MS (MS/MS). As expected, some proteins were differentially expressed in root hairs compared with roots (e.g., a chitinase and phosphoenolpyruvate carboxylase). Out of 37 spots analyzed by MS/MS, 27 candidate proteins were identified by database comparisons. These included several proteins known to respond to rhizobial inoculation (e.g., peroxidase and phenylalanine-ammonia lyase). However, novel proteins were also identified (e.g., phospholipase D and phosphoglucomutase). This research establishes an excellent system for the study of root-hair infection by rhizobia and, in a more general sense, the functional genomics of a single, plant cell type. The results obtained also indicate that proteomic studies with soybean, lacking a complete genome sequence, are practical.

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mentioning

confidence: 99%

Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

Wan¹,

Torres²,

Ganapathy³

et al. 2005

MPMI

124

122

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“…The four-order structure for the phylogenetic position of P. indica (after Schüßler et al 2001;Varma et al 2001) "dialogue" between plant and fungus, as exerted through the exchange of both recognition and acceptance signals (Vierheilig and Piché 2002). The result of this dialogue finally depends on the genome expression of both partners (Gianinazzi-Pearson et al 1996;Bestel-Corre et al 2004;Franken and Requena 2001;Kuster et al 2007).…”

Section: Functions Of Am Fungimentioning

confidence: 96%

Mycorrhizal Fungi and Other Root Endophytes as Biocontrol Agents Against Root Pathogens

et al. 2008

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“…Early studies focussed on the investigation of model mycorhizal and rhizobial symbioses (Bestel-Corre et al, 2004). Synonymous to other 'omic' approaches, with technical developments proteomics has been applied to investigate community functioning in natural environments.…”

Section: Proteomicsmentioning

confidence: 99%

Genomics and the Rhizosphere

Tett

Turner

Poole

2012

Encyclopedia of Life Sciences

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The rhizosphere is a nutrient‐rich area surrounding roots with increased microbial number and activity compared with bulk soil. Plant–microbe interactions in the rhizosphere are an essential determinant of plant health and productivity, as well as biogeochemical cycling. Traditionally, genomics has focussed on the small proportion of cultivable microbes, but recent advances in culture‐independent molecular tools, high‐throughput deoxyribonucleic acid (DNA) sequencing, and bioinformatics are revolutionising our understanding of plant–microbe interactions. Identification of total DNA (metagenomics), ribonucleic acid (RNA) (metatranscriptomics) and expressed proteins (metaproteomics) and metabolites (metabolomics) are now applicable to complex microbial communities such as those in the rhizosphere. Combining these genomic technologies will enable us to analyse the rhizosphere at the systems level. Key Concepts: Soil microbial diversity is vast and plant interfaces are a focus of microbial activity. Plant–microbial interactions are an essential determinant of plant health and productivity. Microbes in the rhizosphere play a key role in biogeochemical cycling. Genomic technologies enable the investigation of the genetic and functional diversity of noncultivable microbes. Next generation sequencing technologies are revolutionising the study of microbial community analysis.

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Proteomics as a tool to monitor plant-microbe endosymbioses in the rhizosphere

Cited by 45 publications

References 95 publications

Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

Mycorrhizal Fungi and Other Root Endophytes as Biocontrol Agents Against Root Pathogens

Genomics and the Rhizosphere

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