Proteomics as an Emergent Tool for Identification of Stress-Induced Proteins in Control and Genetically Modified Wheat Lines

Horváth-Szanics, E.; Szabó, Z. G.; Janáky, Tamás; Pauk, János; Hajós, Gy.

doi:10.1365/s10337-005-0708-3

Cited by 21 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study concluded that the proteomic approach is powerful for investigating protein changes in GM crops and for understanding events involved in quality trait. Indeed, Horvath-Szanics et al (2006) used proteomic methods to identify stress-induced proteins in herbicide-resistant GM wheat lines and found changed level of LMW seed proteins and sensitivity to drought stress in this GM wheat under drought stress.…”

Section: Wheatmentioning

confidence: 99%

Proteomic evaluation of genetically modified crops: current status and challenges

Gong¹,

Wang²

2013

Front. Plant Sci.

View full text Add to dashboard Cite

Hectares of genetically modified (GM) crops have increased exponentially since 1996, when such crops began to be commercialized. GM biotechnology, together with conventional breeding, has become the main approach to improving agronomic traits of crops. However, people are concerned about the safety of GM crops, especially GM-derived food and feed. Many efforts have been made to evaluate the unintended effects caused by the introduction of exogenous genes. “Omics” techniques have advantages over targeted analysis in evaluating such crops because of their use of high-throughput screening. Proteins are key players in gene function and are directly involved in metabolism and cellular development or have roles as toxins, antinutrients, or allergens, which are essential for human health. Thus, proteomics can be expected to become one of the most useful tools in safety assessment. This review assesses the potential of proteomics in evaluating various GM crops. We further describe the challenges in ensuring homogeneity and sensitivity in detection techniques.

show abstract

Section: Wheatmentioning

confidence: 99%

Proteomic evaluation of genetically modified crops: current status and challenges

Gong¹,

Wang²

2013

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Furthermore, high temperature applied from anthesis to maturity decreased the contents of the albumin and globulin fractions, while drought applied after anthesis decreased the albumin and globulin fraction, but had no significant effect on gliadins and glutenins 17. Several studies have been performed to investigate the response of wheat grain proteins to either long‐term drought during vegetative growth or long‐term high temperature applied during grain filling, showing up‐regulation of pathogenesis‐related proteins, allergens, protease inhibitors, heat shock proteins and proteins involved in oxidative stress and starch biosynthesis 14, 18, 19. However, little is known about the effect of the combination of short‐term (days) water deficits and high temperature during grain development on wheat grain proteins.…”

Section: Introductionmentioning

confidence: 99%

Implications of high‐temperature events and water deficits on protein profiles in wheat (Triticum aestivumL. cv. Vinjett) grain

et al. 2011

View full text Add to dashboard Cite

Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are reported of interaction of water deficits and/or a high-temperature event (32°C) during vegetative growth (terminal spikelet) with either of these stress events applied during generative growth (anthesis) in wheat. Influence of combinations of stress on protein fractions (albumins, globulins, gliadins and glutenins) in grains and stress-induced changes on the albumin and gliadin proteomes were investigated by 2-DE and MS. The synthesis of individual protein fractions was shown to be affected by both the type and time of the applied stresses. Identified drought or high-temperature-responsive proteins included proteins involved in primary metabolism, storage and stress response such as late embryogenesis abundant proteins, peroxiredoxins and α-amylase/trypsin inhibitors. Several proteins, e.g. heat shock protein and 14-3-3 protein changed in abundance only under multiple high temperatures.

show abstract

“…Thus, in spite of the aforementioned advantages of a gel-free protein separation method coupled to MS, a differential expression proteomics strategy that combines 2-DGE and MS is still the dominant analytical platform to investigate how the genetic modification produce alterations in proteins abundance, structure, or function, as well as to study the relationships between stress-induced proteins and up-or down-regulated proteins. In this sense, 2-DGE followed by MALDI-TOF MS was used to study the effect of drought stress on the proteomic expression of a herbicide-resistant transgenic wheat; drought affected the expression of low MW proteins, in the range of 15-27 kDa with isoelectric points between 6.5 and 7.5 (Horvath-Szanics et al, 2006). A similar expression proteomics study was performed to assess changes in the transgenic leaf proteome from leaves of GM tobacco with perturbed polyamine metabolism caused by an S-adenosylmethionine decarboxylase (AdoMetDC) overexpression (Franceschetti et al, 2004).…”

Section: Ms Methodologies For Gm Crops Characterizationmentioning

confidence: 99%

MS‐based analytical methodologies to characterize genetically modified crops

García‐Cañas

Simó

León

et al. 2010

Mass Spectrometry Reviews

View full text Add to dashboard Cite

The development of genetically modified crops has had a great impact on the agriculture and food industries. However, the development of any genetically modified organism (GMO) requires the application of analytical procedures to confirm the equivalence of the GMO compared to its isogenic non-transgenic counterpart. Moreover, the use of GMOs in foods and agriculture faces numerous criticisms from consumers and ecological organizations that have led some countries to regulate their production, growth, and commercialization. These regulations have brought about the need of new and more powerful analytical methods to face the complexity of this topic. In this regard, MS-based technologies are increasingly used for GMOs analysis to provide very useful information on GMO composition (e.g., metabolites, proteins). This review focuses on the MS-based analytical methodologies used to characterize genetically modified crops (also called transgenic crops). First, an overview on genetically modified crops development is provided, together with the main difficulties of their analysis. Next, the different MS-based analytical approaches applied to characterize GM crops are critically discussed, and include "-omics" approaches and target-based approaches. These methodologies allow the study of intended and unintended effects that result from the genetic transformation. This information is considered to be essential to corroborate (or not) the equivalence of the GM crop with its isogenic non-transgenic counterpart.

show abstract

Proteomics as an Emergent Tool for Identification of Stress-Induced Proteins in Control and Genetically Modified Wheat Lines

Cited by 21 publications

References 19 publications

Proteomic evaluation of genetically modified crops: current status and challenges

Proteomic evaluation of genetically modified crops: current status and challenges

Implications of high‐temperature events and water deficits on protein profiles in wheat (Triticum aestivumL. cv. Vinjett) grain

MS‐based analytical methodologies to characterize genetically modified crops

Contact Info

Product

Resources

About