Proteomics in Clinical Research: Perspectives and Expectations

Lefkovits, Ivan; Grussenmeyer, Thomas; Matt, Peter; Grapow, Martin; Lefkovits, Michael; Zerkowski, Hans‐Reinhard

doi:10.1002/0470034092.ch3

Cited by 2 publications

(2 citation statements)

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“…The second dimension then separates the proteins according to their molecular mass. 5 The size separation is done in a polyacrylamide matrix in a sodium dodecylsulfate milieu (eg, sodium dodecylsulfate-polyacrylamide gel electrophoresis). Proteins can be visualized in the 2-dimensional gel by using different detection methods.…”

Section: Two-dimensional Gel Electrophoresis and Liquid Chromatograph...mentioning

confidence: 99%

“…Methods for establishing amino acid sequences by means of sequential cleavage of each consecutive N-terminal amino acid were devised decades ago. 5 When it became apparent that the mass of small peptides can be most accurately measured by means of mass spectrometry and the amino acid composition can be deduced from the mass data, considerable effort was invested in adopting methods of working with "true" polypeptides. Because the prerequisite for such analysis is conversion of the analyte into gas-phase ions, proteins must be cleaved into peptidic fragments and then converted into gas-phase ions and ejected to measure the time of flight.…”

Section: Mass Spectrometrymentioning

confidence: 99%

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Proteomics in cardiovascular surgery

Matt

Carrel

White

et al. 2007

The Journal of Thoracic and Cardiovascular Surgery

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Proteomics describes, analogous to the term genomics, the study of the complete set of proteins present in a cell, organ, or organism at a given time. The genome tells us what could theoretically happen, whereas the proteome tells us what does happen. Therefore, a genomic-centered view of biologic processes is incomplete and does not describe what happens at the protein level. Proteomics is a relatively new methodology and is rapidly changing because of extensive advances in the underlying techniques. The core technologies of proteomics are 2-dimensional gel electrophoresis, liquid chromatography, and mass spectrometry. Proteomic approaches might help to close the gap between traditional pathophysiologic and more recent genomic studies, assisting our basic understanding of cardiovascular disease. The application of proteomics in cardiovascular medicine holds great promise. The analysis of tissue and plasma/serum specimens has the potential to provide unique information on the patient. Proteomics might therefore influence daily clinical practice, providing tools for diagnosis, defining the disease state, assessing of individual risk profiles, examining and/or screening of healthy relatives of patients, monitoring the course of the disease, determining the outcome, and setting up individual therapeutic strategies. Currently available clinical applications of proteomics are limited and focus mainly on cardiovascular biomarkers of chronic heart failure and myocardial ischemia. Larger clinical studies are required to test whether proteomics may have promising applications for clinical medicine. Cardiovascular surgeons should be aware of this increasingly pertinent and challenging field of science.

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Section: Two-dimensional Gel Electrophoresis and Liquid Chromatograph...mentioning

confidence: 99%

Section: Mass Spectrometrymentioning

confidence: 99%