Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages

Fan, Wenqiang; Ge, Gentu; Liu, Yinghao; Wang, Wei; Liu, Liying; Jia, Yushan

doi:10.1186/s12870-018-1291-8

Cited by 72 publications

(56 citation statements)

References 47 publications

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“…By using XCMS software, the nonlinear alignment, automatic integration, extraction of the peak intensities, peak alignment, and data filtering were finished. All the metabolites were identified by accuracy mass (< 25 ppm) matching and secondary mass spectrogram matching, and the score cutoff was set as 0.8 [31]. MetaboAnalyst 4.0 was used for statistical analysis [32].…”

Section: Discussionmentioning

confidence: 99%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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Drought is a major abiotic stress that restricts plants growth, development, and yield. Coronatine (COR), a mimic of JA-Ile, functions in plant tolerance to multiple stresses. In our study, we examined the effects of COR in tobacco under polyethylene glycol (PEG) stress. COR treatment improved plant growth under stress as measured by fresh weight (FW) and dry weight (DW). The enzyme activity assay indicated that, under osmotic stress conditions, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were enhanced by COR treatment. Histochemical analyses via nitrotetrazolium blue chloride (NBT) and 3,3′-diaminobenzidine (DAB) staining showed that COR reduced reactive oxygen species (ROS) accumulation during osmotic stress. Metabolite profiles revealed that COR triggered significant metabolic changes in tobacco leaves under osmotic stress, and many essential metabolites, such as sugar and sugar derivatives, organic acids, and nitrogen-containing compounds, which might play active roles in osmotic-stressed tobacco plants, were markedly accumulated in the COR-treated tobacco. The work presented here provides a comprehensive understanding of the COR-mediated physiological, biochemical, and metabolic adjustments that minimize the adverse impact of osmotic stress on tobacco.

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Section: Discussionmentioning

confidence: 99%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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“…using a UHPLC (1290 Infinity LC, Agilent Technologies) coupled to a quadrupole time-of-flight (AB Sciex TripleTOF 6600) in Shanghai Applied Protein Technology Co., Ltd. According to Wenqiang Fan's method, the HILIC separation was carried out samples were analyzed by using a 2.1 mm × 100 mm ACQUIY UPLC BEH 1.7 µm column (waters, Ireland) 69 . In the ESI positive and negative mode, the mobile phase contained A = 25 mM ammonium acetate and 25 mM aqueous ammonium hydroxide, B = acetonitrile.…”

Section: Lc-ms/ms Analysis For Serum and Cecal Contents Determinatiomentioning

confidence: 99%

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Chen

Wang

et al. 2020

Sci Rep

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This study investigated the cecal microbiota and serum metabolite profile of chickens fed with plant essential oils (PEO) or virginiamycin (VIRG) using high-throughput 16S rRNA gene sequencing and untargeted metabolomics approach. the main aim of this work was to explore the biochemical mechanisms involved in the improved growth performance of antibiotics and their alternatives in animal production. The results showed that both PEO and VIRG treatment significantly increased the relative abundance of phyla Bacteroidetes and decreased the abundance of phyla firmicutes and genus of Lactobacillus in cecal microbiota of chickens. compared to the control group (ct group), the relative abundance of genus of Alistipes, unclassified Rikenellaceae, Roseburia, and Anaeroplasma was enriched in the peo group; that of genus Bacteroides, Lachnospiraceae, and unclassified Enterobacteriaceae was enriched in the cecal microbiota of the ViRG group. Untargeted metabolomics analyses revealed that the PEO treatment modified 102 metabolites and 3 KEGG pathways (primary bile acid biosynthesis and phenylalanine metabolism) in the cecal microbiota, and 81 metabolites and relevant KEGG pathways (fructose and mannose metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid.) in the serum of the chicken. Compared to the CT group, VIRG treatment group differed 217 metabolites and 10 KEGG pathways in cecal contents and 142 metabolites and 7 KEGG pathways in serum of chickens. pearson's correlation analysis showed that phyla Bacteroidetes and genus of Bacteroides, Alistipes, and unclassified Rikenellaceae (in the VIRG and PE group) were positively correlated with many lipid metabolites. However, phyla firmicutes and genera Lactobacillus (higher in the ct group) were negatively correlated with the lipid and thymine metabolism, and positively correlated with hydroxyisocaproic acid, cytosine, and taurine. this study shows that dietary supplementation with peo and VIRG altered the composition and metabolism profile of the cecal microbiota, modified the serum metabolism profile. The intestinal microbiota, the population of microorganisms that inhabit the intestine, plays an important role in the intestinal morphology, immunity, nutrient digestion and absorption, and host health 1-3. Many studies have demonstrated that intestinal microbiota participates in many metabolic pathways, such as lipid metabolism and amino acid synthesis 4,5. The mechanism by which PEO promote growth of may be alter gut microflora and hence improved absorption of nutrients 6 , increase absorption of micronutrients in the small intestine 7 and reducing the deleterious effects of the microbial metabolites 8-10. There are many alternatives to antibiotics, such as acidifiers, probiotics, oligosaccharides, and plant extracts, which play a growth promoting role by regulating gut microbes in pig and poultry 11. Plant essential oils (PEO), which can be extracted from plants by steam distillation, extrusion, or solvent extraction 12,13 , serve as alternatives for antibi...

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“…Alanine, purines and pyrimidines content was reduced before the protein synthesis, which could lead to protein synthesis decreased, which was the reason of CP content in solid stage is lower than the jointing stage. The content of phenylalanine increased, but the protein content did not increase in the related metabolic pathway [18]. It can be considered that phenylalanine mainly comes from the hydrolysis of protein, which may also be one of the reasons for the decrease of protein content.…”

Section: Metabolomics Study Of Leymus Chinensismentioning

confidence: 93%

Base on metabolomics:analysis of the internal causes of nutrient changes in Leymus chinensis at different harvest stage

Wang

Zhou

et al. 2019

Preprint

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Background Leymus chinensis is one of most valuable forage on the native grassland for its higher nutrition content. But the local farmer often gained the worse Leymus chinensis hay due to the unfavourable harvest stage, the nutrition of Leymus chinensis was decreasing from jointing stage to solid stage fro its growth characteristics, and the results showed that the crude protein (CP) decreased and the fiber content increased which will caused the nutrition of forage reduced. However, the reason for the nutrient changes in Leymus chinensis has not yet explained at the metabolic level. Results In this research, the Leymus chinensis was harvested at two growth stage(jointing stage and solid stage).Metabolomics was used to analyze the expression changes of metabolites in the whole plant of Leymus chinensis at different harvest stage, and the metabolites were analyzed based on LC-MS/MS untargeted metabolomics. A total of 55 metabolic pathways and 119 metabolites were related to the nutritional change in Leymus chinensis. Most of the metabolites are involved in metabolic processes, including amino acid metabolism, carbohydrate metabolism and acid metabolism biosynthesis. Under the action of EBF1/2 and EIN3, cysteine participates in the ubiquitin-mediated protein degradation pathway. In solid stage, the increasing content of cysteine directly promotes the ubiquitin-mediated protein degradation rate, which is manifested reduce the synthesis of crude protein (CP) in Leymus chinensis, resulting in the decreasing of Leymus chinensis nutrient quality. Carbohydrate metabolism provides raw materials for the synthesis of hemicellulose, leading to the increasing of hemicellulose. In addition, phenylalanine provides the necessary conditions for the synthesis of lignin. These are the internal factors that lead to the decline of quality of Leymus chinensis. Conclusions This study analyzed the elucidate the relationship between the reduction in the nutritional value of Leymus chinensis and complex biological processes by the metabolomic,This study will provides a theoretical basis for producing high-quality Leymus chinensis hay and sets the stage for further research.

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Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages

Cited by 72 publications

References 47 publications

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Base on metabolomics:analysis of the internal causes of nutrient changes in Leymus chinensis at different harvest stage

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