Proteomics study reveals that the dysregulation of focal adhesion and ribosome contribute to early pregnancy loss

Abstract

PurposeEarly pregnancy loss (EPL) affects 50–70% pregnant women in first trimester. The precise molecular mechanisms underlying EPL are far from being fully understood. Therefore, we aim to identify the molecular signaling pathways relating to EPL.Experimental designWe performed proteomics and bioinformatics analysis of the placental villi in women who have undergone EPL and in normal pregnant women. The proteomics data were validated by Western blot analysis.ResultsWe identified a total of 5952 proteins in placental villi, of which 588 proteins were differentially expressed in the EPL women. Bioinformatics analysis revealed that these differentially expressed proteins participated in a variety of signaling pathways, including the focal adhesion pathway and ribosome pathway. Moreover, results of the Western blot confirmed that Desmin, Lamin A/C, MMP‐9, and histone H4 were upregulated in EPL and the Lamin C/ Lamin A ratio decreased obviously in EPL. These proteins could be associated with the pathophysiology of EPL. The data have been deposited to the ProteomeXchange with identifier PXD002391.Conclusion and clinical relevanceOur study demonstrated that the focal adhesion pathway and ribosome pathway are involved in EPL, and these findings might contribute to unveil the pathophysiology of EPL.

Biochemical clinical factors associated with missed abortion independent of maternal age

“…Further bioinformatics analysis indicated that these differentially expressed proteins participated in a variety of signaling pathways, including the focal adhesion pathway and ribosome pathway. [33] These identified proteins, especially those with known roles in apoptosis and oxidative stress, may be novel predictive markers for MA.…”

High Level of APOA1 in Blood and Maternal Fetal Interface Is Associated With Early Miscarriage

“…Liu et al 13 used sliver-stained 2-DE profiling and found 18 differentially expressed proteins, of which 13 were downregulated and 5 were upregulated. Similarly, Xin et al 14 differentially using gel-free TMT labeling. Hence, differential protein expression studies in the EM patients have been of great interest.…”

“…Liu et al 13 used sliver-stained 2-DE profiling and found 18 differentially expressed proteins, of which 13 were downregulated and 5 were upregulated. Similarly, Xin et al 14 used tandem mass tag (TMT) labeling-based proteomics and identified 5952 differentially expressed proteins in placental villi of EM patients compared to controls. Ni et al 15 identified 3423 proteins and reported 51 proteins expressed differentially using gel-free TMT labeling.…”

“…In recent years, various proteomic approaches have been employed to identify altered protein in chorionic villi of EM patients. [13][14][15] We employed comparative expression profiling by Two-dimensional gel electrophoresis (2-DE) followed by image analysis of the Coomassie stained gel. Out of the top most candidates that were differentially expressed between EM patients and willful medical termination of pregnancy, we selected APOA1 for further exploration.…”

Early pregnancy factor, chaperonin 10 and rheumatoid arthritis; the story unravels

“…In 6 studies [21][22][23][24][25] only one study identified one Hsp10 peptide [26], although 100-800 other proteins were identified in these studies. Hsp10 is not intrinsically difficult to detect by mass spectroscopy [27,28]. Using an antibodycapture ELISA (detection limit 0.5 ng/ml) [29], Cpn10 levels were mostly at or below the detection limit, with no indication that levels in mouse pregnancy were significantly elevated ( Figure 2).…”