Proteomics unravels platelet function

Peter, Karlheinz

doi:10.1182/blood-2010-02-270298

Cited by 4 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Proteomic technology has become an indispensable and efficient tool in biochemical research. And, this technology has successfully used to study signal pathways in platelets [12], [13]. Twenty possible target-related proteins of SB in platelets were found in the present study.…”

Section: Discussionmentioning

confidence: 66%

Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B

Yao

Yue

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

BackgroundSalvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin α2β1. But, the signal cascades in platelets after SB binding are still not clear.Methodology/Principal FindingsIn the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin α2β1 was predicted. To certify the predicted network, binding affinity of SB to integrin α2β1 was checked in vitro and ex vivo in platelets. Furthermore, the effects of SB on protein levels of hsp70, coronin-1B and intracellular levels of Ca(2+) and reactive oxygen species (ROS) were checked with or without pre-treatment of platelets using antibody against integrin α2β1. Electron microscopy study confirmed that SB affected cytoskeleton structure of platelets.Conclusions/SignificanceIntegrin α2β1 might be one of the direct target proteins of SB in platelets. The signal cascades network of SB after binding with integrin α2β1 might include regulation of intracellular Ca(2+) level, cytoskeleton-related proteins such as coronin-1B and cytoskeleton structure of platelets.

show abstract

Section: Discussionmentioning

confidence: 66%

Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B

Yao

Yue

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Активація тромбоцитів лежить в основі виконання ними своїх функцій (Peter, 2010;Rao, 2013;Pitchford, 2015). Багато речовин, які входять до списку основних речовин, що активують тромбоцити, можуть синтезуватися за гіпокінезії, особливо тривалої (Stogov, 2009).…”

Section: а бunclassified

Mechanism of damage to platelet and erythrocyte hemostasis in rats with prolonged hypokinesia

Pоpеl

Kryzаnivskaya

Zhurakivskyi

et al. 2017

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

The aim of the study was to determine the morphological changes in the cellular elements of blood in rats of different ages under conditions of prolonged hypokinesia. Research into the structural and functional properties of platelets and erythrocytes was carried out by electron microscope and by biochemical methods in 90 mature male rats aged 2, 12 and 24 months. We found that in young (2 month animals) there was a significant increase in the relative content of activated platelets while the normal content of aggregated and degranulated forms with the appearance of single platelets was maintained with an imbalance of alpha and delta granules. In 12-month-old animals, platelet hemostasis disorders were manifested by a significant increase in the relative content of activated platelets (by 125.8–134.7%) with an increase in aggregated and degranulated forms, the appearance of numerous platelets with an imbalance of alpha- and delta-granules, and reduction of mitochondria (by 24.9–27.8%). In 24 month old animals there was a sharp violation of platelet hemostasis due to a significant increase in the content of activated, degranulated and aggregated platelets, the release of the majority of granules, the development of intravascular platelet hyperactivation, an increase in the number of reversible and irreversibly transformed erythrocytes, an increase in the level of aggregation, in particular the appearance of complex cellular aggregates and bizarre forms of red blood cells. The greatest degree of manifestation of morpho-functional changes was revealed in animals aged 24 months, with relative stability of the hemostasis system in 2 and 12 month old animals. Our results showed that irrespective of age, the degree of disturbance of platelet-erythrocyte hemostasis rose as the period of hypokinesia increased and also depended on the increase in the level of serum creatinine. Taken together, intravascular platelet hyperactivation, an imbalance in the content of all types of granules, the transformation of surface cytoarchitectonics of erythrocytes, and the formation of platelet aggregates may play the role of an early predictor of the development of hypokinetic disease

show abstract

“…Other recent applications of proteomics to platelet biology have provided hints of information that may be relevant to transfusion medicine. These include the report that platelets may continue to divide during storage [33] or the identification of novel signalling components during glycoprotein VI‐induced platelet activation as analysed by differential proteomics [34,35].…”

Section: Platelet Proteomicsmentioning

confidence: 99%

Proteomic applications in blood transfusion: working the jigsaw puzzle

Devine

Schubert

2010

Vox Sanguinis

View full text Add to dashboard Cite

The application of proteomic technologies to transfusion medicine has opened new avenues to our understanding of the products we prepare for patients and the processes that impact the quality of those products. The development of the field of proteomics has paralleled that of transfusion medicine with over a century of key scientific accomplishments required to bring us to our modern systems. We review the technology of proteomics and its application to transfusion medicine with specific reference to the analysis of blood products, both fractionated and fresh. Although the use of proteomic tools to address transfusion medicine questions is really just beginning, it is clear that this method of analysis provides different insights into unaddressed issues in the area of blood product research. Proteomics also offers the promise of improving our approach to the control of blood product quality and even the assessment of blood donors, but these are efforts for the near future.

show abstract

Proteomics unravels platelet function

Cited by 4 publications

References 8 publications

Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B

Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B

Mechanism of damage to platelet and erythrocyte hemostasis in rats with prolonged hypokinesia

Proteomic applications in blood transfusion: working the jigsaw puzzle

Contact Info

Product

Resources

About