Proteotranscriptomics reveals the secretory dynamics of teratocytes, regulators of parasitization by an endoparasitoid wasp

Pinto, Ciro Pedro G.; Walker, Andrew A.; Robinson, Samuel D.; King, Glenn F.; Rossi, Gabriel Augusto Marques

doi:10.1016/j.jinsphys.2022.104395

Cited by 6 publications

(11 citation statements)

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“…We selected four ICK peptides reported to be abundantly produced by the teratocytes of C. flavipes (Pinto et al. , 2022) for production and characterization (Table 1). Three smaller putative ICKs (CftICK‐I, CftICK‐II, and CftICK‐III) were produced by SPPS.…”

Section: Resultsmentioning

confidence: 99%

“…, 2019; Pinto et al. , 2022). Here, we studied the bioactivity of four C. flavipes teratocyte‐derived peptides produced in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…Parasitoids recruit ICK peptides for host regulation (Parkinson et al, 2004), but so far, their function in a parasitism context have not been elucidated (Özbek et al, 2019;Pinto et al, 2022). Here, we studied the bioactivity of four C. flavipes teratocyte-derived peptides produced in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…Peptides were expressed in the periplasm of E. coli using the optimized protocols described by Saez et al (2017). Sequence information obtained in a previous study (Pinto et al, 2022) was used to design codonoptimized genes that were produced by gene synthesis and subcloned in between the KpnI and XhoI restriction sites of a pLIC-MBP vector as a commercial service by GeneART (Thermo Fisher Scientific, Waltham, MA, USA). Peptides were expressed with an N-terminal MalE signal sequence to direct them into the periplasm (Cabrita et al, 2006;Saez et al, 2017).…”

Section: Recombinant Expressionmentioning

confidence: 99%

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Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)

et al. 2022

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Teratocytes are specialized cells released by parasitoid wasps into their hosts. They are known for producing regulatory molecules that aid the development of immature parasitoids. We have recently reported the primary structures of cystine-rich peptides, including some containing inhibitor cystine knot (ICK) motifs, produced by teratocytes of the parasitoid Cotesia flavipes (Hymenoptera: Braconidae). ICKs are known for their stability and diverse biological functions. In this study, we produced four putative ICK peptides from the teratocytes of C. flavipes using solid-phase peptide synthesis or recombinant expression in E. coli, and investigated their functions on host immune modulation as well their potential to impair the development of two lepidopterans after ingestion of the peptides. In addition, the peptides were assayed against pathogens and human cells. The peptides did not influence total hemocyte count but suppressed cellular immunity, detectable as a reduction of hemocyte encapsulation (CftICK-I, CftICK-II, CftICK-III) and spread indexes (CftICK-IV) in the host. None of the peptides influenced the activities of prophenoloxidase and phenoloxidase in the hemolymph of larval Diatraea saccharalis (Lepidoptera: Crambidae). CftICK-I and CftICK-II with previously unknown function showed antifungal activity against Candida albicans but were non-toxic to human cells. CftICK-I, CftICK-II, and CftICK-III increased larval mortality and reduced leaf consumption of D. saccharalis, a permissive host for C. flavipes. The CftICK-III also increased larval mortality and reduced leaf consumption of Spodoptera frugiperda (Lepidoptera: Noctuidae), a non-permissive host for C. flavipes. This study highlights biological functions and biotechnological potential of ICK peptides from the teratocytes of C. flavipes.

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Section: Resultsmentioning

confidence: 99%

“…, 2019; Pinto et al. , 2022). Here, we studied the bioactivity of four C. flavipes teratocyte‐derived peptides produced in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Recombinant Expressionmentioning

confidence: 99%

See 2 more Smart Citations

Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)

et al. 2022

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“…Thus, endoparasitoids require the ability to overcome the host immune response for successful parasitism. For this, endoparasitoids inject maternal factors such as polydnavirus (PDV), venom, and teratocytes into the host along with the eggs (Lin et al 2019; Pinto et al 2022). Suppression of the host immune response by maternal factors has been reported in several endoparasitoid‐host relationships.…”

Section: Introductionmentioning

confidence: 99%

Identification of immune‐related genes from the transcriptome of the venom gland in Cotesia plutellae

Cha,

Na,

Lee

2023

Entomological Research

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Cotesia plutellae, an endoparasitoid wasp is the natural enemy against the diamondback moth, Plutella xylostella. Successful parasitization of C. plutellae requires maternal factors such as polydnavirus, venom proteins, and ovary proteins into the host, protecting their eggs from the host's immune system. To identify the immune‐related genes from the venom glands, we analyzed the transcriptome of venom glands via RNA‐seq. RNA‐seq analysis reveals that 39,207 transcripts were identified. Totally 106 immune‐related genes such as peptidoglycan‐recognition proteins, serpins, spätzles, phenoloxidase, defensin, and calreticulin, etc. were identified and classified into pathogen recognition, immune signal modulation, Toll pathway, IMD pathway, effectors, and other immune genes, depending on their biological functions. These results suggest that the transcriptome of the venom glands of C. plutellae can expand the understanding of insect immune response.

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Silicon Application Enhances Sugarcane Growth by Impairing the Development of Larval Sugarcane Borer

Sousa,

Pinto,

dos Santos

et al. 2023

Silicon

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Sugarcane borer, Diatraea saccharalis, is one of the main insect pests of sugarcane elds, and it has been mainly managed by the use of chemical or biological controls. Considering the bene ts of Silicon (Si) fertilization against pests, it was assessed the development of sugarcane borer larvae and sugarcane growth with and without Si. A greenhouse experiment was conducted using a factorial design (2x2) with 10 repetitions. Two commercial sugarcane varieties were evaluated: SP80-3280 and IAC91-1099, which has, respectively, susceptibility, and intermediate resistance to D. saccharalis. Si was applied in soil in an equivalent rate of 800 kg of Si ha − 1 . Before herbivory, Si increased stalk diameter and plant height in both varieties, and number of leaves and leaf width were only increased in IAC91-1099. After 20 days of herbivory, Si increased stalk diameter in both varieties and plant height in IAC91-1099, but decreased the number of leaves and leaf width in SP80-3280. Larval D. saccharalis showed a reduced weight and a greater index for mandible abrasion after feeding Si-treated plants independently of variety. No in uence of Si-treated plants was found in immunological parameters of larvae (total number of hemocytes, cell viability, encapsulation capability, lysozyme active). The activity of phenol oxidase, an immunological and stress marker for insects, was greater in larval D. saccharalis fed with IAC 91-1099, independently of Si. In conclusion, Si application improved sugarcane growth of IAC91-1099 and impaired the development of larval D. saccharalis in both sugarcane varieties.

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Proteotranscriptomics reveals the secretory dynamics of teratocytes, regulators of parasitization by an endoparasitoid wasp

Cited by 6 publications

References 87 publications

Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)

Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)

Identification of immune‐related genes from the transcriptome of the venom gland in Cotesia plutellae

Silicon Application Enhances Sugarcane Growth by Impairing the Development of Larval Sugarcane Borer

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