Proteus mirabilis fimbriae (PMF) are important for both bladder and kidney colonization in mice

Zunino, Pablo; Sosa, Vanessa; Allen, Andrew G.; Preston, Andrew; Schlapp, Geraldine; Maskell, Duncan J.

doi:10.1099/mic.0.26534-0

Cited by 54 publications

(49 citation statements)

References 25 publications

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“…Our data also indicate that uropathogenesis of P. mirabilis may be a multifactorial process in which different virulence factors can act in a concerted or even redundant fashion, since absence of different factors did not abolish the colonization of the lower and upper urinary tract in mice (Zunino et al, 2000(Zunino et al, , 2001(Zunino et al, , 2003Legnani-Fajardo et al, 1996;Bahrani et al, 1994;Massad et al, 1994).…”

Section: Discussionmentioning

confidence: 90%

“…Although the experimental ascending UTI model in mice has been extensively used to evaluate the role of different P. mirabilis virulence factors (Zunino et al, 2000(Zunino et al, , 2001(Zunino et al, , 2003Legnani-Fajardo et al, 1996;Bahrani et al, 1994;Massad et al, 1994;Jones et al, 1990;Swihart & Welch, 1990), so far, it has not been used to assess the potential infectivity of P. mirabilis isolates from diverse sources. Different groups of mice were transurethrally challenged with each isolate.…”

Section: Experimental Ascending Uti In Micementioning

confidence: 99%

“…We determined that all isolates expressed MR/P and PMF fimbriae. These two particular types of fimbriae are frequently related to P. mirabilis UTI pathogenesis (Zunino et al, 2001(Zunino et al, , 2003Bahrani et al, 1994;Massad et al, 1994).…”

Section: Pcr Amplification and Expression Of Fimbrial Genesmentioning

confidence: 99%

“…Whole-cell preparations obtained from 48 h static cultures in LB broth were run on SDS-PAGE and proteins were transferred to nitrocellulose membranes (Bio-Rad) as described by Towbin et al (1979). Western immunoblots were performed using a 1 : 200 dilution in PBS/Tween 20/1 % (w/v) skimmed milk of rabbit polyclonal immune sera raised against MrpA (Zunino et al, 2001), PmfA (Zunino et al, 2003) and AtfA (Zunino et al, 2000), and mouse polyclonal immune sera raised against UcaA (Scavone et al, 2004). Isogenic fimbrial mrpA, pmfA, atfA-C and ucaA mutants were included as controls in the Western blotting assays (strains MSD2, P2, A4 and UM1 respectively; see Table 1).…”

Section: Sds-pagementioning

confidence: 99%

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Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

2006

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Proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (UTIs). In this study, a collection of P. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. Isolates were characterized in vitro, assessing several virulence properties that had been previously associated with P. mirabilis uropathogenicity. Swarming motility, urease production, growth in urine, outer-membrane protein patterns, ability to grow in the presence of different iron sources, haemolysin and haemagglutinin production, and the presence and expression of diverse fimbrial genes, were analysed. In order to evaluate the infectivity of the different isolates, the experimental ascending UTI model in mice was used. Additionally, the Dienes test and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay were performed to assess the genetic diversity of the isolates. The results of the present study did not show any correlation between distribution of the diverse potential urovirulence factors and isolate source. No significant correlation was observed between infectivity and the origin of the isolates, since they all similarly colonized the urinary tract of the challenged mice. Finally, all isolates showed unique ERIC-PCR patterns, indicating that the isolates were genetically diverse. The results obtained in this study suggest that the source of P. mirabilis strains cannot be correlated with pathogenic attributes, and that the distribution of virulence factors between isolates of different origins may correspond to the opportunistic nature of the organism.

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Section: Discussionmentioning

confidence: 90%

Section: Experimental Ascending Uti In Micementioning

confidence: 99%

Section: Pcr Amplification and Expression Of Fimbrial Genesmentioning

confidence: 99%

Section: Sds-pagementioning

confidence: 99%

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Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

2006

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“…These strains possess MR/P, PMF, and UCA fimbriae, flagella and produce hemolysin [16][17][18][19][20]. P. mirabilis strains were stored at -80 ºC in Luria-Bertani (LB) broth supplemented with 12% (v/v) glycerol and grown aerobically at 37 ºC.…”

Section: Methodsmentioning

confidence: 99%

Effect of Ibicella lutea on uropathogenic Proteus mirabilis growth, virulence, and biofilm formation

Sosa

Zunino

2009

J Infect Dev Ctries

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Background: Proteus mirabilis, an important uropathogen that can cause complicated urinary tract infections (UTI), has emerged as a therapeutic problem following mutations that compromise the use of antimicrobial drugs. Due to the serious effects associated with uropathogenic P. mirabilis and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for its control. The objective of this study was to assess the effect of Ibicella lutea extract, a South American indigenous plant, on growth, virulence and biofilm production of uropathogenic P. mirabilis. Methodology: This study was based on the extract generation and the assessment of its effect on bacterial features related to virulence. These assays involved determination of antibacterial activity, swarming motility, Western blot to assess expression of fimbriae and flagella, biofilms formation, haemagglutination, haemolysis, and electron microscopy. Results and Conclusions: I. lutea extract had an effect on bacterial growth rate and bacterial morphology. It also affected P. mirabilis swarming differentiation, hemagglutination and biofilm formation on glass and polystyrene. These findings suggest that I. lutea may have a role as an agent for the control of P. mirabilis UTI.

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Encrustation of Indwelling Urinary Devices

Chan

Lange

2017

Ureteric Stenting

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Proteus mirabilis fimbriae (PMF) are important for both bladder and kidney colonization in mice

Cited by 54 publications

References 25 publications

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Effect of Ibicella lutea on uropathogenic Proteus mirabilis growth, virulence, and biofilm formation

Encrustation of Indwelling Urinary Devices

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