Protocol for efficient micropropagation of spring gentian and sand jurinea

Cvrčková, H.; Máchová, P.; Dostál, J.; Malá, Jana

doi:10.17221/60/2013-jfs

Cited by 1 publication

(1 citation statement)

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“…1h). In many studies, the organic elicitors are regulating the biochemical entities which have prolific influence on plant growth and multiple shoot development as they control vital cellular processes including DNA replication, fruit development, senescence and secondary metabolite production under abiotic and biotic stress conditions (Baskaran and Van Staden, 2011;Cvrckova et al 2014).…”

Section: Multiple Shoots Proliferation With Organic Elicitorsmentioning

confidence: 99%

Effects of carbendazim on <em>In vitro</em> micropropagation and flowering of pepper elder herbal plant

Ahmed

Lydia

Musthafa

et al. 2019

Trop Agric Res & Ext

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The present study reports on application of carbendazim for micropropagation and in vitro flowering of Peperomia pellucida. In vitro multiplication of shoot tips and axillary nodal explants were used in 25-100 mgL -1 carbendazim in 70% alcohol treatment for 5 min in MS (Murashige and Skoog) medium and MS hormone medium The explants were cultured on MS medium supplemented with auxins, cytokinins and additives (Casin hydrolyte and glutamine) used to proliferate the shoots. The maximum number of shoots (21.5/shoot tip) were developed on MS medium supplemented with 2.0 mgL -1 KIN (Kinetin) 0.5 mgL -1 NAA (α-Naphthalene acetic acid), 100 mgL -1 casein hydrolysate and 100 mgL -1 glutamine additives. The highest number of roots per shoot (17.6) and highest rooting frequency (94.2%) were obtained when the adventitious shoots were inoculated on MS medium with 1.5 mgL -1 IAA (Indole-3-acetic acid). The highest number of shoot development (75.4 %) was achieved on MS medium supplemented with 2.0 mgL -1 KIN 2.0 + 0.5 mgL -1 ABA (Abscisic acid) with 5% sucrose. It was found that MS medium devoid of 1.5 mgL -1 IAA promote the root formation and maturation of in vitro flower in P. pellucida. Acclimatized plantlets were transferred to botanical garden and the characters compared with mother plant of P. pellucida.

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Section: Multiple Shoots Proliferation With Organic Elicitorsmentioning

confidence: 99%