Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification

Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

“…For MS sample preparation, our optimized protocol was followed 89 , 90 with the exception that acetone precipitation, which was used given the small volume/amount of sample. Seven times the sample volume of ice-cold acetone was added, and the samples were precipitated overnight.…”