Protocol to detect in vitro and in cell ubiquitylation of mitochondrial DNA polymerase gamma by mitochondrial E3 ligase MITOL

Hyperubiquitylation of DNA helicase RECQL4 by E3 ligase MITOL prevents mitochondrial entry and potentiates mitophagy

“…Protocols for both in vitro and in cell ubiquitylation for PolγA by MITOL have recently been described ( 45 ). In vitro deubiquitylation assays using USP30 were carried out as described in ( 20 , 46 ).…”