Protocols for sperm cryopreservation in the domestic cat: A review

Buranaamnuay, Kakanang

doi:10.1016/j.anireprosci.2017.06.002

Cited by 34 publications

(31 citation statements)

References 71 publications

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“…La técnica de recolecta seminal por cateterismo uretral fue desarrollada inicialmente utilizando como modelo experimental al gato doméstico (Zambelli et al, 2008;Zambelli, Raccagni, Cunto, Andreani, & Isani, 2010). La técnica proporciona una opción rápida en comparación con la técnica por vagina artificial (Buranaamnuay, 2017), menor costo económico en equipo y una menor contaminación con orina en comparación con la técnica de electroejaculación en felinos (Lueders, Luther, Scheepers, & van der Horst, 2012;Pukazhenthi & Wildt, 2004), adicionalmente el cateterismo uretral ofrece menores complicaciones para la obtención de la muestra en comparación con la recolecta seminal del epidídimo (Filliers, et al, 2010). De esta forma, el cateterismo uretral posibilita la obtención de un mayor número de eyaculados con un proceso simplificado.…”

Section: Discussionunclassified

Biometría testicular y características seminales en felinos neotropicales (Carnivora: Felidae) sometidos a cateterismo uretral

Madrigal‐Valverde¹

2019

RBT

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Introducción: La administración de de un agonista de un fármaco alfa- 2-adrenérgicos asociada con la técnica del cateterismo uretral , es un método de desarrollo reciente en la colección seminal en felinos, ofreciendo las ventajas sobre la colecta por electroeyaculación y la colecta epididimária. La técnica se ha probado en Panthera onca y felinos de pequeño porte. Métodos: En este estudio se utilizaron individuos de Panthera onca , Puma yagouaroundi y Leopardus wedii en cautiverio en el parque Zoobotánico Getúlio Vargas, Salvador, Bahia, Brasil. Fue utilizado los farmácos de clorhidrato de medetomidina (0,1mg /kg) y cetamina (5mg /kg) y fue colectado semen por medio del cateterismo uretral. Durante el desarrollo del procedimiento, se realizó la colecta de los datos biométricos corporales y testiculares, así como la ultrasonografáa de los testículos. Las muestras seminales frescas fueron evaluadas los parámetros de volumen, por microscopía convencional, el vigor espermático, motilidad total, motilidad progresiva, la integridad estructural y funcional de la membrana plasmática y la morfología espermática. Las muestras se sometieron a un proceso de congelación - descongelación utilizando tres diferentes crioprotectores. Las muestras descongeladas fueron evaluadas por microscopía convencional, análisis seminal computarizado y citometría de flujo. Resultados: En este estudio fue realizado con éxito la técnica de colección seminal en las tres especies, siendo el primer estudio que informa de los parámetros seminales de P. yagouaroundi y el primero que reporta la colección seminal uretral en L. wiedii. Los datos presentados describen la morfocinética espermática de las especies incluidas de forma pionera. No hubo diferencias (P>0,05) en la calidad seminal evaluando por grupo de crioprotector, ni diferencias en la integridad de las membranas pré e post-congelamento. Discusión: Foi posible solamente la comparación con otras técnicas de colección seminal o con resultados de calidad seminal obtenidos de muestras de gato doméstico. El establecimiento de una potencial asociación de las características físicas del animal y la calidad seminal, es información de suma importancia en la aplicabilidad para la elección de reproductores en los programas de reproducción asistida.

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Section: Discussionunclassified

Biometría testicular y características seminales en felinos neotropicales (Carnivora: Felidae) sometidos a cateterismo uretral

Madrigal‐Valverde¹

2019

RBT

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“…Cryopreservation protocols for domestic cats describe glycerol (4% or 5%) and egg yolk (10-20%) associated with the diluents TRIS (tris(hydroxymethyl)aminomethane) or TEST (N-tris-hydroxymethyl-methyl-2-aminomethane) as cryoprotectants (Buranaamnuay, 2017). Although egg yolk is an excellent extracellular cryoprotectant, there are controversies about its use; it has been reported as having substances that hinder cellular respiration, affecting the electron transport system in the mitochondria of the spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of Aloe vera extracts in cold storage media and cryopreservation of domestic cat epididymal spermatozoa

et al. 2020

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This study evaluated the effect of the extract of Aloe vera at concentrations of 10% and 20% on the cryopreservation of sperm from the epididymis of domestic cats. Epididymal spermatozoa were recovered using the flotation technique and used in the treatments: control (TRIS-egg yolk at 20%), T10% (TRIS plus 10% of A. vera extract), and T20% (TRIS plus 20% of A. vera extract). The spermatozoa were subjected to 4ºC for 60 minutes, followed by 20 minutes in nitrogen vapors, and stored in a cryogenic cylinder. The samples were thawed at 37°C for 30 seconds. The sperm motility decreased (P<0.05) after thawing in the three treatments. Only the spermatozoa in the control treatment maintained postthawing vigor. The viability of spermatozoa decreased in the treatments with A. vera (P<0.05). According to the hypoosmotic test, all treatments maintained the sperm membrane functionality (P>0.05) during freezing; however, after thawing, it decreased (P<0.05) in the T10% and T20% treatments. The morphology and chromatin condensation of spermatozoa did not differ, regardless of the treatments and time of evaluation (P>0.05). The effect of the crude A. vera extract was not satisfactory on the cryopreservation of epididymal spermatozoa of domestic cats after thawing; although the motility of spermatozoa was similar to that found with the use of egg yolk, and it presented maintenance of the chromatin integrity. However, it is necessary to understand the action of the substances present in A. vera with the feline spermatozoa, well as the standardization and adjustment of physicochemical characteristics aiming at the future application of the vegetal extract.

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“…Cryopreservation allows for a long‐term storage of genetic materials facilitating offspring propagation (Chatdarong, ), especially in valuable cat breeds and endangered wild animals (Buranaamnuay, ; Zambelli et al, ). Nevertheless, freezing/thawing process reduces survival and fertilising ability of spermatozoa, and it induces a significant decrease in motility, viability, acrosome integrity (Luvoni, ; Terrell et al, ; Thiangtum, Pinyopummin, Hori, Kawakami, & Tsutsui, ) and lowers sperm metabolism by decreasing mitochondrial function in cat spermatozoa (Prochowska, Niżański, & Partyka, ; Terrell et al, ; Thuwanut, Arya, Comizzoli, & Chatdarong, ).…”

Section: Introductionmentioning

confidence: 99%

Changes in sperm function and structure after freezing in domestic cat spermatozoa

et al. 2018

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Sperm cryopreservation allows for a long-term storage of genetic. However, changes due to factors as cold shock, osmotic and oxidative stress cause reduction in viability and fertilising ability of frozen/thawed spermatozoa. Therefore, evaluation of cryoinjury of cat spermatozoa is a key factor in achieving better cryopreservation results. This study analysed the changes in structural and functional after freezing in ejaculated domestic cats spermatozoa. Semen samples (n = 60) were analysed before and after freezing, progressive motility was determined with computer-assisted sperm analysis and viability, and acrosome intact spermatozoa, mitochondrial function and superoxide anion ( ) were assessed by flow cytometry. The results demonstrated that cryopreservation induced changes in all sperm parameters (p< 0.05). Total sperm motility, viability, acrosome integrity and mitochondrial function of fresh samples were near to 80% and decrease near to 40% in frozen/thawed spermatozoa (p < 0.05); nevertheless, in contrast to all other sperm parameters, the sperm positive with increased post/thawing (p< 0.05). In conclusion, changes in frozen/thawed spermatozoa could be related to the effect of oxidative stress due to the increase in the synthesis of and a concomitant loss of functional competence. Therefore, the evaluation of these sperm parameters could contribute to complement the analysis of fresh or frozen semen used ART.

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Protocols for sperm cryopreservation in the domestic cat: A review

Cited by 34 publications

References 71 publications

Biometría testicular y características seminales en felinos neotropicales (Carnivora: Felidae) sometidos a cateterismo uretral

Biometría testicular y características seminales en felinos neotropicales (Carnivora: Felidae) sometidos a cateterismo uretral

Effect of supplementation of Aloe vera extracts in cold storage media and cryopreservation of domestic cat epididymal spermatozoa

Changes in sperm function and structure after freezing in domestic cat spermatozoa

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