Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin

Rahmani, Mohammad-Shafie; Pijut, Paula M.; Shabanian, Naghi

doi:10.1007/s11240-016-1072-8

Cited by 26 publications

(18 citation statements)

References 67 publications

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“…MS full- and half-strength media have been successfully used to induce callus formation from protoplast-derived microcalli in several plant species, including petunia species [ 13 , 16 , 21 , 28 ]. However, previous studies did not investigate the role of different culture media in callus formation.…”

Section: Discussionmentioning

confidence: 99%

“…Of all treatment combinations, 1.0 mg/L BA and 0.2 mg/L IBA is the most suitable for shoot regeneration from a protoplast-derived callus. Genetic stability of in vitro regenerated plants has been detected in several plant species using RAPD marker [ 22 , 28 , 29 , 30 ]. In this study, as RAPD analysis showed occurrence of the same banding patterns in protoplast-derived shoots compared with those in donor plants.…”

Section: Discussionmentioning

confidence: 99%

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Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Kang

Naing

Kim

2020

Biology

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Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in Petunia hybrida cv. Mirage Rose. The following conditions were found to be most optimal for protoplast yield and viability: 0.6 M mannitol, 2.0% cellulase, and 6 h digestion time. A plating density of 10 × 104 protoplasts/mL under osmoticum condition (0.58 M mannitol) showed high microcolony viability in liquid culture. The Kao and Michayluk medium was found to be appropriate for callus proliferation from microcalli under a 16-h light photoperiod. Calli cultured in Murashige and Skoog medium containing 1.0 mg/L 6-benzylaminopurine and 0.2 mg/L 3-indole butyric acid showed the highest shoot regeneration frequency and number of shoots obtained per explant. Random amplification of polymorphic DNA analysis showed that the protoplast-derived shoots exhibited the same banding patterns as those of donor plants. Collectively, these findings can contribute to solving problems encountered in protoplast isolation and shoot regeneration in other petunia cultivars and related species. As the protocol developed by us is highly reproducible, it can be applied in biotechnology research on P. hybrida cv. Mirage Rose.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Kang

Naing

Kim

2020

Biology

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“…The second method provides tools to avoid hybridization barriers related to sexual reproduction and can allow development of hybrids from more distantly related species (Eeckhaut et al 2013;Kuligowska et al 2016). The ability to isolate high numbers of healthy protoplasts enables a successful protoplast culture and development of an efficient protoplast-to-plant system (Rahmani et al 2016). However, its establishment is influenced by many factors, among others: genotype, physiological status and growing conditions of the protoplast source tissue, protoplast isolation and medium composition (Davey et al 2005;Eeckhaut et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Zeatin is frequently combined with NAA and used in protoplast regeneration protocols for shoot induction e.g. in Solanum (Borgato et al 2007) and Albizia julibrissin (Rahmani et al 2016). Moreover, zeatin has a positive effect on shoot regeneration from leaf-derived callus of K. blossfeldiana (Varga et al 1988).…”

Section: Discussionmentioning

confidence: 99%

“…In the future research further efforts are needed to accomplish the increase in the shoot regeneration efficiency. The optimal medium supplementation with PGRs for shoot regeneration from protoplast-derived callus varies depending on the plant species and even genotypes (Davey et al 2005;Rahmani et al 2016). Sanikhani et al (2006) and Lütken et al (2011) reported successful shoot regeneration in Kalanchoë blossfeldiana by TDZ.…”

Section: Discussionmentioning

confidence: 99%

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Protoplast isolation and culture from Kalanchoë species: optimization of plant growth regulator concentration for efficient callus production

Chen

Mackenzie

Eeckhaut

et al. 2019

Plant Cell Tiss Organ Cult

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A high yield of isolated protoplasts and efficient regeneration protocols are prerequisites for successful development of somatic hybrids. In the present study, protoplast isolation and regeneration were evaluated in 12 Kalanchoë accessions belonging to nine species. The highest protoplast yield was obtained from K. blossfeldiana 'Charming Red Meadow' with 10.78 ± 0.51 × 10 5 protoplasts per gram fresh weight. We observed significant differences of protoplast yield while there was no distinct difference in viability among the accessions. Seven accessions reached the microcolony stage and four developed microcalli in medium supplemented with 1.0 mg/l 1-naphthaleneacetic acid (NAA), 0.5 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D). Using five selected accessions we optimized the PGR (plant growth regulators) concentrations using combinations of NAA, BAP and 2,4-D. K. blossfeldiana cultivars 'Charming Red Meadow' and 'Paris' produced significantly different numbers of calli depending on the PGR concentrations. For plant regeneration, the medium was supplemented with 1 mg/l NAA and 2 mg/l BAP or 2 mg/l zeatin. Shoots were regenerated on medium supplemented with NAA and BAP for K. blossfeldiana 'Charming Red Meadow' and K. blossfeldiana 'Paris'. The plants successfully developed roots on the medium supplemented with IAA. The medium containing zeatin induced root formation directly from callus in K. blossfeldiana 'Charming Red Meadow'. Our findings have the potential to facilitate the use of Kalanchoë species in somatic hybridization breeding programs. Key MessageThe study revealed a strong genotype-dependent efficiency of colony and microcallus formation. Plants were regenerated from two Kalanchoë blossfeldiana cultivars on medium supplemented with NAA and BAP.

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Hairy Root Culture for the Production of Useful Secondary Metabolites

Abraham

Thomas

2017

Biotechnology and Production of Anti-Cancer Compounds

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Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin

Cited by 26 publications

References 67 publications

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Protoplast isolation and culture from Kalanchoë species: optimization of plant growth regulator concentration for efficient callus production

Hairy Root Culture for the Production of Useful Secondary Metabolites

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