Prototype 3D-Printed Traps Capture Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) Directly into Preservative for Improved Detection of “Candidatus Liberibacter solanacearum”

Wentz, Katie; Cooper, William C.; Horton, David; Wohleb, Carrie H.; Waters, Tim; Halbert, Susan E.; Ramadugu, Chandrika; Snyder, James; Kao, Robert M.

doi:10.18474/0749-8004-55.2.147

Cited by 4 publications

(10 citation statements)

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“…In pilot studies, we reported that propylene glycol with salt (NaCl) was suitable for ACP preservation and pathogen detection in stored ACPs for up to six weeks [ 26 ]. Using a previous model of the stem trap designed and printed by the Division of Plant Industry, similar studies were conducted with the tomato psyllid, Bactericera cockerelli ; the psyllids were well preserved and suitable for pathogen testing [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…All the components were printed using a 3D printer in the stem traps, except the clear plastic dome at the top and the capture tubes attached to the funnel. A previous version of the stem traps described here was used for the trapping of the tomato psyllid, Bactericera cockerelli [ 22 , 24 ]; the stem trap described in the current manuscript has many novel features and modifications compared to the one described earlier in the tomato psyllid study.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, adhesive traps have a high level of non-target by-catch, are very messy, difficult to handle, and are inconvenient to use in the field and the laboratory [ 22 , 23 ]. Due to degradation of ACPs captured on sticky cards, the DNA extracted is of lower quality compared to other preservation methods [ 24 ]. Alternative trapping systems that are easy to deploy, reduce non-target by-catch, do not generate the sticky mess, and preserve specimens for molecular analysis are valuable [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Due to degradation of ACPs captured on sticky cards, the DNA extracted is of lower quality compared to other preservation methods [ 24 ]. Alternative trapping systems that are easy to deploy, reduce non-target by-catch, do not generate the sticky mess, and preserve specimens for molecular analysis are valuable [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we describe the development of ACP traps for efficient capture in field conditions. The 3D-printed traps provide a method to capture insects that can be tested for the presence of Liberibacter pathogens [ 24 , 26 ]. The traps were evaluated in the field conditions in Florida.…”

Section: Introductionmentioning

confidence: 99%

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The Development and Evaluation of Insect Traps for the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae), Vector of Citrus Huanglongbing

Snyder

Dickens

Halbert

et al. 2022

Insects

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Citrus huanglongbing (HLB) is a severe problem for citrus cultivation. The disease management programs benefit from improved field tools suitable for surveying the ACP vector (Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae)) and the associated pathogen. In the present study, we utilize three-dimensional (3D) printers and design tools to develop traps that can capture and preserve ACPs. Three novel, 3D-printed traps were designed and evaluated: stem trap, and cylinder traps 1 and 2. The traps and yellow sticky cards were deployed weekly for 8 months in 2 non-commercial citrus groves in Florida; in California, the traps were evaluated for 12 months in field cages and 4 citrus groves. The stem traps captured lower numbers of ACPs at all experimental sites compared to the cylinder traps. Capture rates in the cylinder traps were comparable to the sticky trap, making the device a viable tool for monitoring field ACPs. The two main advantages of using the reusable 3D traps over standard methods of ACP and HLB surveys include dynamic sampling that can be conducted year-round and the capture of ACPs that can be preserved and tested. Improved trapping may facilitate quick management decisions and mitigate HLB.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Development and Evaluation of Insect Traps for the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae), Vector of Citrus Huanglongbing

Snyder

Dickens

Halbert

et al. 2022

Insects

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Directed Sequencing of Plant Specific DNA Identifies the Dietary History of Four Species of Auchenorrhyncha (Hemiptera)

Cooper

Marshall

Foutz

et al. 2021

Annals of the Entomological Society of America

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Auchenorrhyncha (Hemiptera) includes several families of sap-feeding insects that tend to feed on a wide-range of host plants. Some species within Auchenorrhyncha are major agricultural pests that transmit plant pathogens or cause direct feeding damage. Nearly all pest Auchenorrhyncha are highly polyphagous, have mobile nymphs, and colonize crops from noncrop habitats. We examined whether methods for gut content analysis developed for more specialized Hemiptera identify dietary history of polyphagous Auchenorrhyncha. We used high-throughput sequencing of the plant genes trnF and ITS to examine the dietary history of Circulifer tenellus (Baker) (Cicadellidae), Colladonus geminatus (Van Duzee) (Cicadellidae), Colladonus montanus reductus (Van Duzee) (Cicadellidae), and Lycorma delicatula (White) (Fulgoridae). C. tenellus is a vector of the vegetable pathogens Candidatus Phytoplasma trifolii and beat curly top virus. Both Colladonus species are vectors of Ca. Phytoplasma pruni, the pathogen associated with X-disease of stone fruits. Lycorma delicatula is an invasive pest of grape and ornamentals in the eastern United States. Results showed spring hosts for C. tenellus included Brassicaceae, especially Sisymbrium sp. (tumble mustard), and spring hosts for both Colladonus species included Taraxacum sp. (dandelion). Gut content analysis also detected a decrease in host-breadth by L. delicatula from early to late instars. Results demonstrate that directed sequencing of plant DNA identified the dietary history of leafhopper and planthopper pests. Expanded use of gut content analysis will help identify the noncrop sources of phytoplasma-infected C. tenellus and Colladonus, and to examine seasonal changes in host shifts by L. delicatula.

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Seasonal activity of Trechnites insidiosus (Hymenoptera: Encyrtidae) and its host Cacopsylla pyricola (Hemiptera: Psyllidae) in pear

Zilnik,

Cooper,

Horton

et al. 2023

Journal of Economic Entomology

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Cacopsylla pyricola (Förster) (Hemiptera: Psyllidae) is the most expensive and challenging insect pest of commercial pear trees in the Pacific Northwest. Integrated pest management (IPM) programs are working toward relying more heavily on natural enemies to reduce insecticide use. Trechnites insidiosus (Crawford) (Hymenoptera: Encyrtidae) is the main parasitoid of C. pyricola, but little is known about its biology in the region. Developing sampling tools is important for the deployment of IPM programs, including monitoring of natural enemies. In this study, we examined 2 conventional monitoring methods: beat trays and yellow sticky cards, in addition to screened sticky cards and 3D-printed cylinder traps. Additionally, we tested an overwintering trap for the collection of parasitized C. pyricola. The trapping methods were tested in orchards in Oregon and Washington. Unscreened cards caught the most T. insidiosus and C. pyricola, followed by screened cards, cylinder traps, and then beat trays. Beat trays sometimes failed to catch any T. insidiosus, even when it was found in abundance via other methods. Screened cards and cylinder traps reduced bycatch and increased ease of identifying T. insidiosus. Specimens from the cylinder traps were also more suitable for use in molecular analysis. The overwintering traps were effective at capturing parasitized C. pyricola, but were highly variable year to year. The ideal trapping method will vary based on research needs (e.g., DNA preservation, reducing bycatch, catching higher numbers), but both screened sticky cards and cylinder traps were viable methods for monitoring T. insidiosus and its host.

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Prototype 3D-Printed Traps Capture Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) Directly into Preservative for Improved Detection of “Candidatus Liberibacter solanacearum”

Cited by 4 publications

References 12 publications

The Development and Evaluation of Insect Traps for the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae), Vector of Citrus Huanglongbing

The Development and Evaluation of Insect Traps for the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae), Vector of Citrus Huanglongbing

Directed Sequencing of Plant Specific DNA Identifies the Dietary History of Four Species of Auchenorrhyncha (Hemiptera)

Seasonal activity of Trechnites insidiosus (Hymenoptera: Encyrtidae) and its host Cacopsylla pyricola (Hemiptera: Psyllidae) in pear

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