Prozac affects stickleback nest quality without altering androgen, spiggin or aggression levels during a 21-day breeding test

Sebire, Marion; Davis, Jessica Elphinstone; Hatfield, Robert G.; Winberg, Svante; Katsiadaki, Ioanna

doi:10.1016/j.aquatox.2015.09.009

Cited by 28 publications

(14 citation statements)

References 95 publications

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“…Our finding that exposure to fluoxetine increased boldness matches findings in previous work on sticklebacks (Grécias et al, 2017) and on other fish species (Wong et al, 2013;Ansai et al, 2016;Singer et al, 2016;Sinyakova et al, 2018). Fluoxetine has been shown to also decrease the predator response in sticklebacks (Sebire et al, 2015) and in the fathead minnow (Painter et al, 2009;Weinberger and Klaper, 2014). Additionally, exposure to a similar serotonin-manipulating pharmaceutical, citalopram, increased boldness in fish (Kellner et al, 2016) and other animals (Bergey et al, 2016;Holtmann et al, 2016).…”

Section: Discussionsupporting

confidence: 90%

“…our combined treatment, the overall drug dose was actually higher than in the single treatments (combined individuals got both the full serotonin and full dopamine doses), this could result in a lower pharmacological response in the combined treatment compared with the single drug treatment. Fluoxetine can affect nest quality in sticklebacks with no concentration response (Sebire et al, 2015), suggesting the existence of a plateau in the dose-response curve. Many studies dealing with the effects of pharmaceuticals in the environment are restricted by the use of only one or a couple of different drug concentrations, thus making it difficult to evaluate whether the observed effects are concentration dependent (Sumpter et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Abbey-Lee

Kreshchenko

Sala

et al. 2019

Journal of Experimental Biology

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Among-individual behavioral differences (i.e. animal personality) are commonly observed across taxa, although the underlying, causal mechanisms of such differences are poorly understood. Animal personality has been correlated with physiological functions as well as fitness-related traits. Variation in many aspects of monoamine systems, such as metabolite levels and gene polymorphisms, has been linked to behavioral variation. Therefore, here we experimentally investigated the potential role of monoamines in explaining individual variation in personality, using two common pharmaceuticals that respectively alter the levels of serotonin and dopamine in the brain: fluoxetine and ropinirole. We exposed threespined sticklebacks, a species that shows animal personality, to either chemical alone or to a combination of the two chemicals, for 18 days. During the experiment, fish were assayed at four time points for the following personality traits: exploration, boldness, aggression and sociability. To quantify brain gene expression on short-and longer-term scales, fish were sampled at two time points. Our results show that monoamine manipulations influence fish behavior. Specifically, fish exposed to either fluoxetine or ropinirole were significantly bolder, and fish exposed to the two chemicals together tended to be bolder than control fish. Our monoamine manipulations did not alter the gene expression of monoamine or stress-associated neurotransmitter genes, but control, untreated fish showed covariation between gene expression and behavior. Specifically, exploration and boldness were predicted by genes in the dopaminergic, serotonergic and stress pathways, and sociability was predicted by genes in the dopaminergic and stress pathways. These results add further support to the links between monoaminergic systems and personality, and show that exposure to monoamines can causally alter animal personality.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Abbey-Lee

Kreshchenko

Sala

et al. 2019

Journal of Experimental Biology

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“…The authors suggest that fin-clipping is an area that could offer the opportunity for refinement if genotyping might be completed without the invasive fin-clip and recently this has been reported (Breacker et al, 2017). There are efforts reported in the scientific community to refine this procedure in other species such as Neolamprologus pulcher (Trewavas & Poll 1952) (Le Vin et al, 2011) and Gasterosteus aculeatus L. 1758 (Sebire et al, 2015). Handling the smaller D. rerio without anaesthesia, however, may prove stressful.…”

Section: A Na E S T H E S I a A N D E U T H A Na S I Amentioning

confidence: 99%

International survey on the use and welfare of zebrafish Danio rerio in research

Lidster

Readman

Prescott

et al. 2017

Journal of Fish Biology

100

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A survey was conducted regarding zebrafish Danio rerio use for scientific research with a focus on: anaesthesia and euthanasia; housing and husbandry; breeding and production; refinement opportunities. A total of 98 survey responses were received from laboratories in 22 countries in Europe, North America, South America, Asia and Australia. There appears a clear and urgent need to identify the most humane methods of anaesthesia and euthanasia. Aversive responses to MS-222 were widely observed raising concerns about the use of this anaesthetic for D. rerio. The use of anaesthesia in fin clipping for genetic identification is widely practised and there appears to be an opportunity to further develop less invasive methods and refine this process. Optimization (and potentially standardization) of feeding is an area for further investigation. Given that diet and body condition can have such profound effects on results of experiments, differences in practice could have significant scientific implications. Further research into transition between dark and light phases in the laboratory appears to represent an opportunity to establish best practice. Plants and gravel were not considered practical by many laboratories. The true value and benefits need to be established and communicated. Overproduction is a concern both from ethical and financial viewpoints. There is an opportunity to further reduce wastage of D. rerio. There are clear concerns and opportunities for the scientific community to work together to further improve the welfare of these important laboratory models.

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“…Fluoxetine, for instance, causes in vitro contraction of gastric muscle through a cholinergic pathway (James et al, 2005) and slows gut movement in rats subjected to a force-swimming test (Xie et al, 2013). Behavioural effects of SSRI exposure have been shown in different fish species, although none of these previous studies have ruled out non-specific effects by using a separate treatment that combines exposure to a SSRI and to a serotonin antagonist to determine if it reverses the observed behavioural effects of SSRI completely (Gaworecki and Klaine, 2008;Wong et al, 2013;Hedgespeth et al, 2014;Sebire et al, 2015). Our results are thus consistent in methodology and findings with previous studies but do not rule out non-specific effects.…”

Section: Limitations and Future Approachesmentioning

confidence: 99%

“…+ ? Present study Hamilton et al, 2016 Present study Gibson and Keenleyside, 1966 Present study Milinski, 1985;Giles, 1987Painter et al, 2009Sebire et al, 2015;Weinberger and Klaper, 2014 Present study Croy and Hughes, 1991;Damsgird and Dill, 1998 Present study…”

Section: Introductionmentioning

confidence: 99%

Can the behaviour of threespine stickleback parasitized with Schistocephalus solidus be replicated by manipulating host physiology?

Hébert

Berger

Barber

et al. 2016

Journal of Experimental Biology

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Sticklebacks infected by the parasitic flatworm Schistocephalus solidus show dramatic changes in phenotype, including a loss of species-typical behavioural responses to predators. The timing of host behaviour change coincides with the development of infectivity of the parasite to the final host (a piscivorous bird), making it an ideal model for studying the mechanisms of infection-induced behavioural modification. However, whether the loss of host anti-predator behaviour results from direct manipulation by the parasite, or is a by-product (e.g. host immune response) or side effect of infection (e.g. energetic loss), remains controversial. To understand the physiological mechanisms that generate these behavioural changes, we quantified the behavioural profiles of experimentally infected fish and attempted to replicate these in non-parasitized fish by exposing them to treatments including immunity activation and fasting, or by pharmacologically inhibiting the stress axis. All fish were screened for the following behaviours: activity, water depth preference, sociability, phototaxis, anti-predator response and latency to feed. We were able to change individual behaviours with certain treatments. Our results suggest that the impact of S. solidus on the stickleback might be of a multifactorial nature. The behaviour changes observed in infected fish might result from the combined effects of modifying the serotonergic axis, lack of energy and activation of the immune system.

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Prozac affects stickleback nest quality without altering androgen, spiggin or aggression levels during a 21-day breeding test

Cited by 28 publications

References 95 publications

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

International survey on the use and welfare of zebrafish Danio rerio in research

Can the behaviour of threespine stickleback parasitized with Schistocephalus solidus be replicated by manipulating host physiology?

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