PrP genotypes in a pedigree flock of Santa Inês sheep

Lima, Arjuman; Bossers, Alex; Souza, Carlos Eduardo A.; Oliveira, Sandra Maria de; Oliveira, De

doi:10.1136/vr.160.10.336

Cited by 14 publications

(29 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Outros estudos envolvendo a raça Santa Inês, Lima et al (2007) mostraram que em 29 amostras genotipadas foram encontrados 9 alelos e 13 genótipos. Os resultados semelhantes demonstram que a raça Santa Inês possui grande variabilidade genética.…”

Section: Discussão E Conclusõesunclassified

“…Levando em consideração que essa combinação não é considerada resistente, ϐica claro que mais da metade do rebanho ovino analisado tem susceptibilidade ao desenvolvimento da doença. De maneira semelhante Sotomaior et al (2008) e Lima et al (2007) em seus estudos veriϐicaram a predominância do genótipo ARQ/ARQ nos animais analisados, sendo que nestas ocasiões foram analisadas amostras de animais criados no Brasil. Os resultados semelhantes demonstram que a raça nativa Santa Inês é potencialmente susceptivel ao scrapie.…”

Section: Discussão E Conclusõesunclassified

“…Em outros estudos realizados envolvendo a raça nacional Santa Inês não foi detectado este genótipo (Lima et al 2007, Sotomaior et al 2008. A baixa frequência do alelo ARR no rebanho nacional reforça a susceptibilidade da raça Santa Inês em relação ao scrapie.…”

Section: Discussão E Conclusõesunclassified

See 2 more Smart Citations

Genotipagem de polimorfismos no gene prnp em ovinos da raça Santa Inês no Estado de São Paulo

et al. 2012

View full text Add to dashboard Cite

Section: Discussão E Conclusõesunclassified

See 1 more Smart Citation

Genotipagem de polimorfismos no gene prnp em ovinos da raça Santa Inês no Estado de São Paulo

et al. 2012

View full text Add to dashboard Cite

“…A survey conducted for this review of more than 20 studies [1,2,4,14,20,21,23,24,27,38,54,64,68,69,82,83,[86][87][88]90] reporting on sheep populations from 15 different countries in Europe, America and Asia revealed that so far all but one (Iceland) presented significant frequencies of all five major alleles (ARQ; ARR; AHQ; VRQ; ARH) in their sheep. Iceland appears not to have any ARR animals [86].…”

Section: Classical Scrapie In Sheepmentioning

confidence: 99%

PrPgenetics in ruminant transmissible spongiform encephalopathies

2008

View full text Add to dashboard Cite

-Scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) are prion diseases in ruminants with considerable impact on animal health and welfare. They can also pose a risk to human health and control is therefore an important issue. Prion protein (PrP) genetics may be used to control and eventually eradicate animal prion diseases. The PrP gene in sheep and other representatives of the order Artiodactyles has many polymorphisms of which several are crucial determinants of susceptibility to prion diseases, also known as transmissible spongiform encephalopathies (TSE). This review will present the current understanding of PrP genetics in ruminants highlighting similarity and difference between the species in the context of TSE.

show abstract

“…Since then, some studies have analyzed variation in codons 136, 154 and 171 of the prion protein (Passos et al 2008, Lima et al 2007, Sotomaior et al 2008), but other codon variations in the PrP gene from Brazilian ovine and goat breeds have not been extensively analyzed.…”

Section: Introductionmentioning

confidence: 99%

Single nucleotide polymorphisms at 15 codons of the prion protein gene from a scrapie-affected herd of Suffolk sheep in Brazil

et al. 2011

View full text Add to dashboard Cite

Scrapie is a transmissible spongiform encephalopathy of sheeps and goats, associated with the deposition of a isoform of the prion protein (PrPsc). This isoform presents an altered conformation that leads to aggregation in the host's central nervous and lymphoreticular systems. Predisposition to the prion agent infection can be influenced by specific genotypes related to mutations in amino acids of the PrPsc gene. The most characterized mutations occur at codons 136, 154 and 171, with genotypes VRQ being the most susceptible and ARR the most resistant. In this study we have analyzed polymorphisms in 15 different codons of the PrPsc gene in sheeps from a Suffolk herd from Brazil affected by an outbreak of classical scrapie. Amplicons from the PrPsc gene, encompassing the most relevant altered codons in the protein, were sequenced in order to determine each animal's genotype. We have found polymorphisms at 3 of the 15 analyzed codons (136, 143 and 171). The most variable codon was 171, where all described alleles were identified. A rare polymorphism was found at the 143 codon in 4% of the samples analyzed, which has been described as increasing scrapie resistance in otherwise susceptible animals. No other polymorphisms were detected in the remaining 12 analyzed codons, all of them corresponding to the wild-type prion protein. Regarding the risk degree of developing scrapie, most of the animals (96%) had genotypes corresponding to risk groups 1 to 3 (very low to moderate), with only 4% in the higher risks group. Our data is discussed in relation to preventive measures involving genotyping and positive selection to control the disease.

show abstract

PrP genotypes in a pedigree flock of Santa Inês sheep

Abstract: SCRAPIE is a prion disease of sheep and goats, and is characterised by the accumulation of pathologically folded prion protein (PrP) derived from host-encoded PrP. For sheep, over 40 polymorphisms of PrP have been described

Cited by 14 publications

References 15 publications

Genotipagem de polimorfismos no gene prnp em ovinos da raça Santa Inês no Estado de São Paulo

Genotipagem de polimorfismos no gene prnp em ovinos da raça Santa Inês no Estado de São Paulo

PrPgenetics in ruminant transmissible spongiform encephalopathies

Single nucleotide polymorphisms at 15 codons of the prion protein gene from a scrapie-affected herd of Suffolk sheep in Brazil

Contact Info

Product

Resources

About