PRP significantly promoted the adhesion and migration of vascular smooth muscle cells on the stent material

Abstract

Background The adhesion and survival state between cells and scaffold material is a major problem in the process of tissue engineered blood vessel (TEBV) culture. Platelet rich plasma (PRP) contains a large amount of biologically active factors and fibrin, which is expected to play an important role in the process of TEBV culture.Purpose To combine PRP with cells and scaffold material to promote cell adhesion and biological activity on the scaffold material.Methods The adhesion status and migration ability of SMC cells under the conditions of optimal concentration suitable for SMC growth and optimal concentration of PRP were examined by scanning electron microscopy, HE staining, CCK8, qPCR, WB and other experimental means, and compared with the situation of conventional culture (20% FBS); finally, the effect of PRP on the deposition of ECM in vascular tissue engineering culture was verified by three-dimensional culture.Results 20% PRP is a more suitable concentration of SMC, compared with the control group, the 20% PRP group has better migration ability, the number of SMC adhesion is significantly higher than the control group, in addition to this, the collagen deposition in the experimental group is significantly higher than the control experimental group.Conclusion 20% PRP can promote the ability of SMC adhesion, migration and collagen deposition on the scaffold material.