Prussian Blue acts as a mediator in a reagentless cytokinin biosensor

Kowalska, Marta; Tian, Faming; Šmehilová, Mária; Galuszka, Petr; Frébort, Ivo; Napier, Richard; Dale, Nicholas

doi:10.1016/j.aca.2011.06.018

Cited by 12 publications

(5 citation statements)

References 40 publications

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“…In addition, the collection of sufficient amounts of material to exceed the detection limit of currently used devices is another major bottleneck. Alternatively, a biosensor system with electrochemical detection could be developed based on ACO activity, as was performed for cytokinin measurements based on cytokinin oxidase (CKX) activity (Kowalska et al. , 2011).…”

Section: Tissue‐ and Cell‐type‐specific Regulation Of Ethylenementioning

confidence: 99%

Ethylene in vegetative development: a tale with a riddle

et al. 2012

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SummaryThe vegetative development of plants is strongly dependent on the action of phytohormones. For over a century, the effects of ethylene on plants have been studied, illustrating the profound impact of this gaseous hormone on plant growth, development and stress responses. Ethylene signaling is under tight self-control at various levels. Feedback regulation occurs on both biosynthesis and signaling. For its role in developmental processes, ethylene has a close and reciprocal relation with auxin, another major determinant of plant architecture. Here, we discuss, in view of novel findings mainly in the reference plant Arabidopsis, how ethylene is distributed and perceived throughout the plant at the organ, tissue and cellular levels, and reflect on how plants benefit from the complex interaction of ethylene and auxin, determining their shape. Furthermore, we elaborate on the implications of recent discoveries on the control of ethylene signaling.

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Section: Tissue‐ and Cell‐type‐specific Regulation Of Ethylenementioning

confidence: 99%

Ethylene in vegetative development: a tale with a riddle

et al. 2012

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“…The study of brain ethanol concentration dynamics in real time, therefore, requires a different approach. In this regard, amperometric biosensors are proving useful as they allow detection of a range of nonelectroactive analytes by virtue of the presence of a biological element (usually an enzyme) that can interact specifically with a target, producing an electroactive byproduct. − In this study, different designs of alcohol biosensors were developed and characterized, where the biological element was alcohol oxidase (AOx; EC 1.1.3.13), a flavoprotein with eight subunits, each containing a flavin adenine dinucleotide (FAD) cofactor molecule that plays a pivotal role in the enzyme activity (reaction ). AOx is capable of catalyzing the oxidation of primary, aliphatic short-chain alcohols (such as ethanol or methanol) to the respective aldehydes (reactions and ). RCH 2 OH + AOx / FAD → RCHO + AOx / FADH 2 AOx / FADH 2 + normalO 2 → AOx / FAD + normalH 2 normalO 2 normalH 2 normalO 2 → normalO 2 + 2 normalH + + 2 normale − …”

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confidence: 99%

Development and Characterization of an Implantable Biosensor for Telemetric Monitoring of Ethanol in the Brain of Freely Moving Rats

et al. 2012

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Ethanol is one of the most widespread psychotropic agents in western society. While its psychoactive effects are mainly associated with GABAergic and glutamatergic systems, the positive reinforcing properties of ethanol are related to activation of mesolimbic dopaminergic pathways resulting in a release of dopamine in the nucleus accumbens. Given these neurobiological implications, the detection of ethanol in brain extracellular fluid (ECF) is of great importance. In this study, we describe the development and characterization of an implantable biosensor for the amperometric detection of brain ethanol in real time. Ten different designs were characterized in vitro in terms of Michaelis-Menten kinetics (V(MAX) and K(M)), sensitivity (linear region slope, limit of detection (LOD), and limit of quantification (LOQ)), and electroactive interference blocking. The same parameters were monitored in selected designs up to 28 days after fabrication in order to quantify their stability. Finally, the best performing biosensor design was selected for implantation in the nucleus accumbens and coupled with a previously developed telemetric device for the real-time monitoring of ethanol in freely moving, untethered rats. Ethanol was then administered systemically to animals, either alone or in combination with ranitidine (an alcohol dehydrogenase inhibitor) while the biosensor signal was continuously recorded. The implanted biosensor, integrated in the low-cost telemetry system, was demonstrated to be a reliable device for the short-time monitoring of exogenous ethanol in brain ECF and represents a new generation of analytical tools for studying ethanol toxicokinetics and the effect of drugs on brain ethanol levels.

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“…For instance, MHCF possesses notable electron mediating ability. A variety of MHCFs have been prepared and used for electrochemical sensors [30][31][32][33][34][35]. We previously described a miniaturized amperometric detector coupled to a microfluidic device using CNTs-cobalt hexacyanoferrate (CoHCF) modified graphite electrode; further a microchip electrophoresis-electrochemistry method was proposed [36].…”

Section: Introductionmentioning

confidence: 99%