Prussian Blue Modified Ferritin as Peroxidase Mimetics and Its Applications in Biological Detection

Abstract

Ferritins are natural nanoscale structures composed with 24 subunits endowed with similar three-dimensional structures. The iron is stored in the form of ferrihydrite phosphate in the hollow spherical ferritin shells. Prussian blue nanoparticles (PBNPs) have been certified a kind of mimetic enzyme with the advantages of stability, high catalytic activity and low prices. In this context, we designed a strategy to synthesize PBNPs of small size using ferritin as template and meanwhile retain the biological properties of ferritin. Our results show the resulting nanostructures (Prussian blue modified ferritin nanoparticles, PB-Ft NPs) got very small size and relatively high catalytic activity, furthermore, PB-Ft NPs successfully combined the intrinsic enzyme mimetic activity of PBNPs and the specificity of ferritin. Peroxidase-like activity which fits well the Michaelis-Menten kinetics was found strongly depending on pH, temperature and the concentration of PB-Ft NPs. Then a sensitive method for glucose detection was developed using glucose oxidase (GOx) and PB-Ft NPs. The consequence of Enzyme-linked immunosorbent assay (ELISA) shows PB-Ft NPs possess both specificity and peroxidase-like activity, which suggests that PB-Ft NPs can be served as a useful reagent in some biological detections.

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

“…Numerous papers (see Table 6) described the ability of PBA-NZ to react with typical ROS-enzyme substrates, such as TMB, ABTS and NADH [169]. PB and PBAs are used successfully in optical biosensors as a result of their PO-like properties [12,16,[169][170][171][172][173][174][175]. The first communications concerning electrochemical reduction of H 2 O 2 on PB-modified glassy carbon electrode were done by Itaya and colleagues [164,165].…”

“…In 2000, this group named PB an "artificial PO" [177]. At the same time, a large number of other scientific groups, especially from China, worked hard on this problem [148,154,[156][157][158][159]166,[168][169][170][171][172][173][174].…”

“…PB and PBAs are used successfully in optical biosensors as a result of their PO-like properties [ 12 , 16 , 169 , 170 , 171 , 172 , 173 , 174 , 175 ]. The first communications concerning electrochemical reduction of H 2 O 2 on PB-modified glassy carbon electrode were done by Itaya and colleagues [ 164 , 165 ].…”

Protein‐Based Artificial Nanosystems in Cancer Therapy

“…Zhang et al reported the synthesis of a nanosized PB‐encapsulated structure using FRT as the reactor where ferrocyanide and ferric ion are mixed together. [ 32 ] While they developed this material as a mimetic peroxidase for glucose detection, the special structure of PB‐encapsulated FRT should also have the capacity of using photothermal conversion for PTT.…”

Magnetic drug delivery systems

“…There have been some reports that naturally occurring ferritin is equivalent to a storage compartment for iron in animal and plant cells [115]. Zhang et al [116] devised a strategy to use ferritin as a template for the synthesis of small, Prussian blue-modified ferritin nanoparticles (PB-Ft NPs), which retain the biological characteristics of ferritin. Their results showed that the PB-Ft NPs have a mean size of 22.8 nm, and retain specificity and peroxidase-like activity.…”