Ps1294 Multifactorial Hepcidin Suppression in Beta-Thalassemia Carriers With Iron Overload

Abstract

renal insufficiency. Furthermore, the question raises if the TRP metabolism has a potential link to iron deficiency (ID) or iron deficiency anemia (IDA). This issue is not covered in the current literature. Aims: This prospective study aimed at evaluating the potential association between iron metabolism and hemoglobin (Hb) concentrations and the TRP metabolism in a large cohort of patients sub-grouped by the presence or absence ID or anemia. Methods: In this prospective study, 430 patients, who were admitted by general practitioners and specialists to the outpatient clinic of the Institute of Clinical Chemistry and Laboratory Medicine of the General Hospital Steyr (Steyr, Austria) for a medical check-up of their actual iron status, were included. All participants provided their written informed consent. They underwent venous blood sampling after an overnight fasting state in the morning (between 8.00 and 10.00 a.m.). The samples were used to investigate the iron metabolism (i.e., Hb, mean corpuscular volume [MCV], mean corpuscular hemoglobin [MCH], ferritin, transferrin saturation [TSAT], serum iron, transferrin, soluble transferrin receptor [sTfR], reticulocyte hemoglobin [CHr]), and also the TRP metabolism (TRP, kynurenine [KYN], kynurenic acid [KYNA], KYN/ TRP ratio, KYNA/KYN index). Serum concentrations of TRP, KYN, and KYNA were measured by high-pressure liquid-chromatography Results: Serum TRP, KYN and KYNA concentrations were positively correlated with Hb (p-values <0.001, 0.029, and <0.001) and ferritin (p-values 0.033, 0.008, and <0.001) measurements. In total, 159 patients with ID had significantly lower median (interquartile range) TRP (58.