PSAT1 prompted cell proliferation and inhibited cell apoptosis in multiple myeloma through regulating PI3K/AKT pathway

Zhu, Hongqing; Si, Yejun; Yun, Zhong; Li, Meng; Ji, Jian-Min; Jiang, Ou

doi:10.4314/tjpr.v19i4.10

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…PSAT1 catalyzes the conversion of 3-phosphohydroxypyruvate to phosphoserine, which is further used in the formation of serine. Downregulation of PSAT1 promotes growth inhibition and induces apoptosis [ 41 ]. Cells with high PSAT1 levels increase the ratio of reduced GSH (glutathione) to oxidized GSSG (Glutathione disulfide) and lower ROS.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of Metabolic Subtypes of Endometrial Cancer Using a Systems-Level Approach

Srivastava

Vinod

2023

Metabolites

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Endometrial cancer (EC) is the most common gynecological cancer worldwide. Understanding metabolic adaptation and its heterogeneity in tumor tissues may provide new insights and help in cancer diagnosis, prognosis, and treatment. In this study, we investigated metabolic alterations of EC to understand the variations in metabolism within tumor samples. Integration of transcriptomics data of EC (RNA-Seq) and the human genome-scale metabolic network was performed to identify the metabolic subtypes of EC and uncover the underlying dysregulated metabolic pathways and reporter metabolites in each subtype. The relationship between metabolic subtypes and clinical variables was explored. Further, we correlated the metabolic changes occurring at the transcriptome level with the genomic alterations. Based on metabolic profile, EC patients were stratified into two subtypes (metabolic subtype-1 and subtype-2) that significantly correlated to patient survival, tumor stages, mutation, and copy number variations. We observed the co-activation of the pentose phosphate pathway, one-carbon metabolism, and genes involved in controlling estrogen levels in metabolic subtype-2, which is linked to poor survival. PNMT and ERBB2 are also upregulated in metabolic subtype-2 samples and present on the same chromosome locus 17q12, which is amplified. PTEN and TP53 mutations show mutually exclusive behavior between subtypes and display a difference in survival. This work identifies metabolic subtypes with distinct characteristics at the transcriptome and genome levels, highlighting the metabolic heterogeneity within EC.

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Section: Resultsmentioning

confidence: 99%

Identification and Characterization of Metabolic Subtypes of Endometrial Cancer Using a Systems-Level Approach

Srivastava

Vinod

2023

Metabolites

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“…The WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium-monosodium salt) in the kit could be reduced to highly water-soluble yellow formazan by dehydrogenase under the action of 1-methoxy PMS (1-methoxy-5-methylphenazinonium sulfate) in the cell. The amount of produced formazan was proportional to the number of living cells and could be measured by the absorbance at 450 nm with a microplate reader [ 29 ]. Therefore, cell proliferation could be analyzed by this characteristic.…”

Section: Resultsmentioning

confidence: 99%

Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System

et al. 2022

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Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow delivery of multiple drugs into cells for cell editing, but the biosafety is rarely explored. In this work, a nanostraw-mediated nano-electroporation system was developed, which allowed repeated perforation of the same set of cells in a minimally invasive manner, while the biosafety aspect of this system was investigated. Highly controllable fabrication of Al2O3 nanostraw arrays based on a porous polyethylene terephthalate (PET) membrane was integrated with a microfluidic device to construct the nanostraw-electroporation system. The pulse conditions and intervals of nano-electroporation were systematically optimized to achieve efficient cells perforation and maintain the viability of the cells. The cells proliferation, the early apoptosis activities after nanostraw-electroporation and the changes of gene functions and gene pathways of cells after repeated nano-electroporation were comprehensively analyzed. These results revealed that the repeated nanostraw-electroporation did not induce obvious negative effects on the cells. This work demonstrates the feasibility of repeated nano-electroporation on cells and provides a promising strategy for future biomedical applications.

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“…PSAT1 catalyzes the conversion of 3-phosphohydroxypyruvate to phosphoserine, which is further used in the formation of serine. Downregulation of PSAT1 promotes growth inhibition and induces apoptosis [29]. Cells with high PSAT1 levels increase the ratio of GSH (reduced) to GSSG (oxidized) and lower ROS.…”

Section: Metabolic Gene Alterations In Ecmentioning

confidence: 99%

Identification and characterization of metabolic subtypes of endometrial cancer using systems-level approach

Srivastava

Vinod

2023

Preprint

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Endometrial cancer (EC) is the most common gynaecological cancer worldwide. Understanding the metabolic adaptation and its heterogeneity in tumor tissues may provide new insights and help in cancer diagnosis, prognosis, and treatment. In this study, we investigated metabolic alterations of EC to understand the variations in the metabolism within tumor samples. We integrated the TCGA transcriptomics data of EC (RNA-Seq) with the human genome-scale metabolic model (HMR2.0) and performed unsupervised learning to identify the metabolic subtypes of EC and uncover the underlying dysregulated metabolic pathways and reporter metabolites in each subtype. The relationship between metabolic subtypes and clinical variables was explored. Further, we characterized each subtype at the molecular level and correlated the subtype-specific metabolic changes occurring at the transcriptome level with the genomic alterations. EC patients are stratified into two robust metabolic subtypes (cluster-1 and cluster-2) that significantly correlate to patient survival, tumor stages, mutation, and copy number variations. We observed co-activation of pentose phosphate pathway and one-carbon metabolism along with genes involved in controlling estrogen levels in cluster-2, which is linked to poor survival. PNMT and ERBB2 are also upregulated in cluster-2 samples and present in the same chromosome locus 17q12, which is amplified. PTEN and TP53 mutations show mutually exclusive behavior between subtypes and display a difference in survival. This work identifies metabolic subtypes with distinct characteristics at the transcriptome and genome levels, highlighting the metabolic heterogeneity within EC.

show abstract

PSAT1 prompted cell proliferation and inhibited cell apoptosis in multiple myeloma through regulating PI3K/AKT pathway

Cited by 3 publications

References 17 publications

Identification and Characterization of Metabolic Subtypes of Endometrial Cancer Using a Systems-Level Approach

Identification and Characterization of Metabolic Subtypes of Endometrial Cancer Using a Systems-Level Approach

Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System

Identification and characterization of metabolic subtypes of endometrial cancer using systems-level approach

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