Pseudacidovorax intermedius gen. nov., sp. nov., a novel nitrogen-fixing betaproteobacterium isolated from soil

Kämpfer, Peter; Thummes, Kathrin; Chu, Horng-I; Tan, Chen-Chung; Arun, A. B.; Chen, Wen-Ming; Lai, Wei-An; Shen, Fo-Ting; Rekha, P. D.; Young, Chiu-Chung

doi:10.1099/ijs.0.65175-0

Cited by 28 publications

(9 citation statements)

References 21 publications

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“…As shown in Table S1, the presence of C 16 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) as the major fatty acids of strain CY1 T was similar to related genera. However, strain CY1 T showed a relatively higher content (>10 %) of C 10 : 0 3-OH, which is lower (≤6 %) in Alicycliphilus denitrificans K601 T (this study) and members of the genera Diaphorobacter (Khan & Hiraishi, 2002), Acidovorax (Willems et al , 1990), Delftia (Chen et al , 2012), Comamonas (Tamaoka et al , 1987) and Pseudacidovorax (Kämpfer et al , 2008). The variation in the proportion of C 17 : 0 cyclo and summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ), as well as the absence of C 20 : 0 and summed feature 7 (unknown equivalent chain length 18.846 and/or C 19 : 1 ω6 c ), indicated significant differences of strain CY1 T from the genus Alicycliphilus .…”

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confidence: 51%

Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory

Wang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory A taxonomic study was carried out on strain CY1 T , which is a novel bacterium isolated from wastewater sludge of a melamine-producing factory in Sanming city, Fujian, China. Strain CY1 T was shown to rapidly and completely degrade melamine to NH 3 and CO 2 under aerobic conditions. The isolate was Gram-stain-negative, short-rod-shaped and motile by one unipolar flagellum. Growth was observed at salinities from 0 to 7 % NaCl (optimum, 0.1 %), at temperatures from 15 to 50 6C (optimum, 40-45 6C) and at pH 7-9.5 (optimum pH 9.5). Quinone-8 was detected as the major respiratory quinone. 16S rRNA gene sequence comparisons showed that strain CY1 T was affiliated to the family Comamonadaceae in the class Betaproteobacteria. It was most closely related to members of the genera Alicycliphilus (95.5 %), Diaphorobacter (94.6-95.1 %), Acidovorax (92.9-95.4 %), Delftia (93.0-93.6 %) and Comamonas (92.6-93.9 %). The average nucleotide identity (ANI) values between strain CY1 T and those representing related genera ranged from 84.0 to 86.1 % using Mummer, and from 74.9 to 81.1 % using BLAST. The dominant fatty acids were C 16 : 1 v7c and/or C 16 : 1 v6c, C 16 : 0 , C 10 : 0 3-OH and C 18 : 1 v7c and/or C 18 : 1 v6c, and the major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminophospholipid. The G+C content of the chromosomal DNA was 69.5 mol%. On the basis of the phenotypic and phylogenetic data, strain CY1 T represents a novel species of a new genus, for which the name Melaminivora alkalimesophila gen. nov., sp. nov. is proposed. The type strain of Melaminivora alkalimesophila is CY1 T (5CCTCC AB 2012024 T 5DSM 26006 T ).

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confidence: 51%

Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory

Wang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…Other species found, such as Azohydromonas australica, have been reported as nitrogen-fixing bacteria (Nguyen and Kim 2017), but there are studies regarding the use of this species in the process of manufacturing biodegradable synthetic plastic (Sharma et al 2017). In the case of the bacteria Pseudoacidovorax sp., there was a high similarity with Pseudoacidovorax intermedius, which is also a nitrogen fixing bacterium isolated from the soil (Kampfer et al 2008). Few studies have been performed on these last three species recently described as nitrogen-fixing bacteria.…”

Section: Discussionmentioning

confidence: 96%

Ora-pro-nobis (Pereskia aculeata Mill.) Nutrition as Related to Soil Chemical and Physical Attributes and Plant Growth-Promoting Microorganisms

Vega

Kemmelmeier

Rufini

et al. 2020

J Soil Sci Plant Nutr

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The objective of this study was to evaluate the relationship of nutrients in Ora-pro-nobis to the physical-chemical attributes and the communities of N 2-fixing bacteria and arbuscular mycorrhizal fungi of the soils in the southern part of the state of Minas Gerais, Brazil. Rhizospheric soil, root, and leaf samples were collected from 12 sampling points in four neighboring municipalities. N 2-fixing bacteria from rhizospheric soil, roots, and washed roots macerated in water were isolated using a N-free semisolid medium, and the 16S rRNA gene was sequenced. Arbuscular mycorrhizal fungi spores were extracted from the soil by the wet-sieving method, followed by sucrose-gradient centrifugation. The collected roots were cleared and analyzed according to the traditional methods for the evaluation of mycorrhizal colonization. The soil samples and leaves were analyzed for physicalchemical and chemical attributes, respectively. There was no relationship between the total nitrogen in the soil and the protein content (based on N content) in the leaf of Ora-pro-nobis as well as between the concentration of the other nutrients in the leaf and their corresponding concentration in the soil, except for potassium and magnesium. Of the 86 isolates obtained, 70 were identified in the genus of the N 2-fixing bacteria Azospirillum. There was a variation in spore density and mycorrhizal colonization. The families Ambisporaceae, Archaeosporaceae, Claroideogloraceae, Glomeraceae, Acaulosporaceae, Diversisporaceae, and Gigasporaceae were identified. Associative N 2-fixing bacteria and arbuscular mycorrhizal fungi were abundantly found in close relationship with Ora-pro-nobis, which may explain the ability of this plant to sustain high protein content in its leaves even in soils with relatively low nitrogen concentration.

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“…S9.5) are nitrogen fixers. They may have displaced other genera because of ammonium self-sufficiency [52][53][54] . Thus, the regional pool R might have acted as a 'hotspot'sphin for genus and function selection, which underscores the possibility of selecting desired functions by modifying the conditions of the regional pool.…”

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confidence: 99%

Stabilising microbial communities by looped mass transfer

Abdulkadir

Schattenberg

et al. 2021

Preprint

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Creating structurally and functionally stable microbiomes would be greatly beneficial to biotechnology and human health but so far has proven challenging. Here, we propose a looped mass transfer design that keeps microbiomes constant over long periods of time. The effluent of five parallel reactors that began with the same inoculum, was mixed in a reactor that represented a regional pool. Part of this pool was transferred back to the five reactors. Community dynamics were monitored and visualized by quantitative microbial flow cytometry and selected taxonomic sequencing of whole communities and sorted subcommunities. The rescue effect, known from metacommunity theory, was the main stabilizing mechanism that led to the survival of subcommunities with zero netgrowth, especially at high mass transfer rates. The looped mass transfer approach promises to overcome notorious stochastic structural fluctuations in bioreactors and has the potential to design and stabilize communities that can perform desired functions.

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Pseudacidovorax intermedius gen. nov., sp. nov., a novel nitrogen-fixing betaproteobacterium isolated from soil

Cited by 28 publications

References 21 publications

Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory

Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory

Ora-pro-nobis (Pereskia aculeata Mill.) Nutrition as Related to Soil Chemical and Physical Attributes and Plant Growth-Promoting Microorganisms

Stabilising microbial communities by looped mass transfer

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