Pseudarthrobacter albicanus sp. nov., isolated from Antarctic soil

Abstract

A Gram-stain positive, strictly aerobic, non-motile and rod-shaped strain, NJ-Z5T, was isolated from a soil sample obtained from the Antarctic Peninsula. This strain was taxonomically characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NJ-Z5T belonged to the genus Pseudarthrobacter and showed the highest similarities to Pseudarthrobacter sulfonivorans ALLT (98.07%), followed by Pseudarthrobacter siccitolerans 4J27T (98.00%), Pseudarthrobacter phenanthrenivorans Sphe3T (97.93%) and Pseudarthrobacter psychrotolerans YJ56T (97.82%). The strain was able to grow at 4–28 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, pH 7.0) and with 0–1.0% (w/v) NaCl (optimum, 0%). It had catalase activity but no oxidase activity. The chemotaxonomic characteristics of strain NJ-Z5T, which had MK-9 (H2) as its predominant menaquinone and anteiso-C15:0 (58.5%), anteiso-C17:0 (9.9%) and iso-C16:0 (7.0%) as its major fatty acids, were consistent with classification in the genus Pseudarthrobacter . The polar lipid profile of strain NJ-Z5T comprised phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids and two unidentified phospholipids. The genome of strain NJ-Z5T was 4.57 Mbp with a G+C content of 67.1 mol%. Average nucleotide identity (ANI) values between strain NJ-Z5T and other species of the genus Pseudarthrobacter were found to be low (ANIm <86%, ANIb <80% and OrthoANIu <80 %). Furthermore, digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values between strain NJ-Z5T and the closely related species ranged from 22.7 to 24.0% and from 75.5 to 77.2%, respectively. On the basis of its differential physiological properties, chemotaxonomic characteristics and low ANI, dDDH and AAI results, strain NJ-Z5T is considered to represent a novel species within the genus Pseudarthrobacter , for which the name Pseudarthrobacter albicanus sp. nov. is proposed. The type strain is NJ-Z5T (=CGMCC 1.15636T=KCTC 39722T).

Pseudarthrobacter humi sp. nov., an actinobacterium isolated from soil

“…The major polar lipid profile of RMG13 T was similar to those of the phylogenetically related type strains of species of the genus Pseudarthrobacter . However, the minor polar lipid profile differs from that of the phylogenetically related strains, supporting the hypothesis that the strain represents a novel species of the genus Pseudarthrobacter [6, 42]. The major fatty acid profile of RMG13 T contained anteiso-C 15 : 0 (44.1 %), iso-C 15 : 0 (16.4 %) and anteiso-C 17 : 0 (10.1 %).…”

“…According to LPSN (https://lpsn.dsmz.de/ genus/pseudarthrobacter), the genus Pseudarthrobacter currently comprises 13 species with validly published names. Members of this genus have been isolated from various sources, such as antarctic soil, veterinary clinic materials, sediments, other soils and filtration substrates [5][6][7][8][9][10]. Members of the genus Pseudarthrobacter are Gram-stain-positive, aerobic and rod-or coccusshaped.…”

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

“…Moreover, studies have revealed that members of the genus Pseudarthrobacter can grow under a wide range of temperatures. For example, Pseudarthrobacter albicanus NJ-Z5 T isolated from Antarctic soil has been shown to grow at temperatures ranging from 4 to 28 °C 40 . Similarly, Pseudarthrobacter humi RMG13 T isolated from soil exhibited a capacity to grow within a temperature range of 4–37 °C 41 .…”