We introduce C * -pseudo-multiplicative unitaries and (concrete) Hopf C * -bimodules, which are C * -algebraic variants of the pseudo-multiplicative unitaries on Hilbert spaces and the Hopf-von Neumann-bimodules studied by Enock, Lesieur, and Vallin [5,6,4,10,19,20]. Moreover, we associate to every regular C * -pseudo-multiplicative unitary two Hopf-C *bimodules and discuss examples related to locally compact groupoids.