Pseudodesulfovibrio indicus gen. nov., sp. nov., a piezophilic sulfate-reducing bacterium from the Indian Ocean and reclassification of four species of the genus Desulfovibrio

Cao, Junwei; Gayet, Nicolas; Zeng, Xiang; Shao, Zongze; Jebbar, Mohamed; Alain, Karine

doi:10.1099/ijsem.0.001286

Cited by 75 publications

(51 citation statements)

References 29 publications

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“…Within Deltaproteobacteria, three orders were represented, namely Desulfovibrionales, Desulfobacterales and Syntrophobacterales. OM-RGC.v1.006256245 was most closely related to HgcA from Pseudodesulfovibrio profundus , a strictly anaerobic piezophilic sulfate-reducer bacteria (SRB) previously isolated from marine sediment 18 . It belongs to the Desulfovibrionales order, which contains several members with confirmed Hg-methylating capacity, such as the model species Desulfovibrio desulfuricans with exceptionally high Hg-methylation rates, isolated from estuarine sediments 19 .…”

Section: Resultsmentioning

confidence: 94%

Widespread microbial mercury methylation genes in the global ocean

Villar

Cabrol

2019

Preprint

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11Methylmercury is a neurotoxin that bioaccumulates from seawater to high concentrations in 12 marine fish, putting human and ecosystem health at risk. High methylmercury levels have 13 been found in the oxic subsurface waters of all oceans, yet only anaerobic microorganisms 14 have been identified so far as efficient methylmercury producers in anoxic environments. 15The microaerophilic nitrite oxidizing bacteria Nitrospina has been previously suggested as a 16 possible mercury methylator in Antarctic sea ice. However, the microorganisms processing 17 inorganic mercury into methylmercury in oxic seawater remain unknown. Here we show 18metagenomic evidence from open ocean for widespread microbial methylmercury 19 production in oxic subsurface waters. We find high abundances of the key mercury 20 methylating genes hgcAB across all oceans corresponding to taxonomic relatives of known 21 mercury methylators from Deltaproteobacteria, Firmicutes and Chloroflexi. Our results 22 7 132

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Section: Resultsmentioning

confidence: 94%

Widespread microbial mercury methylation genes in the global ocean

Villar

Cabrol

2019

Preprint

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“…Three orders were represented within Deltaproteobacteria : Desulfovibrionales , Desulfobacterales and Syntrophobacterales . OM‐RGC.v1.006256245 was most closely related to HgcA from Pseudodesulfovibrio profundus , a strictly anaerobic piezophilic sulphate‐reducing bacteria (SRB) previously isolated from marine sediment (Cao et al ., ). OM‐RGC.v1.006256245 belongs to the Desulfovibrionales order, which contains several members with confirmed Hg‐methylating capacity, like the model species Desulfovibrio desulfuricans , which has exceptionally high Hg‐methylation rates (Gilmour et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Widespread microbial mercury methylation genes in the global ocean

Villar

Cabrol

Heimbürger‐Boavida

2020

Environ Microbiol Rep

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Methylmercury is a neurotoxin that bioaccumulates from seawater to high concentrations in marine fish, putting human and ecosystem health at risk. High methylmercury levels have been found in the oxic subsurface waters of all oceans, but only anaerobic microorganisms have been shown to efficiently produce methylmercury in anoxic environments. The microaerophilic nitrite-oxidizing bacteria Nitrospina have previously been suggested as possible mercury methylating bacteria in Antarctic sea ice. However, the microorganisms responsible for processing inorganic mercury into methylmercury in oxic seawater remain unknown. Here, we show metagenomic and metatranscriptomic evidence that the genetic potential for microbial methylmercury production is widespread in oxic seawater. We find high abundance and expression of the key mercury methylating genes hgcAB across all ocean basins, corresponding to the taxonomic relatives of known mercury methylating bacteria from Deltaproteobacteria, Firmicutes and Chloroflexi. Our results identify Nitrospina as the predominant and widespread microorganism carrying and actively expressing hgcAB. The highest hgcAB abundance and expression occurs in the oxic subsurface waters of the global ocean where the highest MeHg concentrations are typically observed.

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“…Quality control of the PMA reaction was carried out using Desulfovibrio aespoeensis (DSM 10631), a bacterial strain isolated from the Äspö HRL groundwater (Motamedi and Pedersen 1998 ), that was recently renamed as Pseudodesulfovibrio aespoeensis (Cao et al 2016 ). Cultures were grown using Desulfobacter medium (DSMZ 193) with strain-specific modifications (2.5 g/L sodium lactate and 1 g/L yeast extract as substrates, incubation at 30°C under anaerobic conditions).…”

Section: Methodsmentioning

confidence: 99%

Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling

López-Fernández

Broman

Turner

et al. 2018

FEMS Microbiology Ecology

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The deep biosphere is the largest ‘bioreactor’ on earth, and microbes inhabiting this biome profoundly influence global nutrient and energy cycles. An important question for deep biosphere microbiology is whether or not specific populations are viable. To address this, we used quantitative PCR and high throughput 16S rRNA gene sequencing of total and viable cells (i.e. with an intact cellular membrane) from three groundwaters with different ages and chemical constituents. There were no statistically significant differences in 16S rRNA gene abundances and microbial diversity between total and viable communities. This suggests that populations were adapted to prevailing oligotrophic conditions and that non-viable cells are rapidly degraded and recycled into new biomass. With higher concentrations of organic carbon, the modern marine and undefined mixed waters hosted a community with a larger range of predicted growth strategies than the ultra-oligotrophic old saline water. These strategies included fermentative and potentially symbiotic lifestyles by candidate phyla that typically have streamlined genomes. In contrast, the old saline waters had more 16S rRNA gene sequences in previously cultured lineages able to oxidize hydrogen and fix carbon dioxide. This matches the paradigm of a hydrogen and carbon dioxide-fed chemolithoautotrophic deep biosphere.

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Pseudodesulfovibrio indicus gen. nov., sp. nov., a piezophilic sulfate-reducing bacterium from the Indian Ocean and reclassification of four species of the genus Desulfovibrio

Cited by 75 publications

References 29 publications

Widespread microbial mercury methylation genes in the global ocean

Widespread microbial mercury methylation genes in the global ocean

Widespread microbial mercury methylation genes in the global ocean

Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling

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