Pseudogene PDIA3P1 promotes cell proliferation, migration and invasion, and suppresses apoptosis in hepatocellular carcinoma by regulating the p53 pathway

Kong, Yahui; Zhang, Lufei; Huang, Yu; He, Tianyu; Zhang, Linshi; Zhao, Xinyi; Zhou, Xin; Zhou, Dongkai; Yan, Yu; Zhou, Jiarong; Xie, Haiyang; Zhou, Lin; Zheng, Shusen; Wang, Weilin

doi:10.1016/j.canlet.2017.07.031

Cited by 59 publications

(45 citation statements)

References 38 publications

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“…Since the hypothesis of ceRNA was developed in 2011 33 , an increasing number of studies have been published to decode the sophisticated RNA network 28,34 . In 2017, Kong et al found that PDIA3P1 knockdown decreased the migration, invasion, and proliferation of HCC cells by affecting the p53 pathway, but the detailed mechanisms were not studied in detail 24 . In other studies, Sun and colleagues demonstrated that PDIA3P1 is overexpressed in OSCC and promotes tumor cell proliferation by absorbing miR-185-5p 25 .…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we analyzed the microarray data of U87MG glioblastoma cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions from the Gene Expression Omnibus (GEO), and identified protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P1), a 2099-bp segment mapping to chromosome 1q21.1, which has been reported highly expressed in HCC and OSCC, upregulated under hypoxic conditions 24,25 . However, the regulation of gene expression and functional mechanisms of PDIA3P1 require further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma

Wang

Gao

et al. 2020

Cell Death Dis

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Hypoxia is a critical factor in the malignant progression of glioma, especially for the highly-invasive mesenchymal (MES) subtype. But the detailed mechanisms in hypoxia-induced glioma MES transition remain elusive. Pseudogenes, once considered to be non-functional relics of evolution, are emerging as a critical factor in human tumorigenesis and progression. Here, we investigated the clinical significance, biological function, and mechanisms of protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P1) in hypoxia-induced glioma MES transition. In this study, we found that PDIA3P1 expression was closely related to tumor degree, transcriptome subtype, and prognosis in glioma patients. Enrichment analysis found that high PDIA3P1 expression was associated with epithelial-mesenchymal transition, extracellular matrix (ECM) disassembly, and angiogenesis. In vitro study revealed that overexpression of PDIA3P1 enhanced the migration and invasion capacity of glioma cells, while knockdown of PDIA3P1 induced the opposite effect. Further studies revealed that PDIA3P1 functions as a ceRNA, sponging miR-124-3p to modulate RELA expression and activate the downstream NF-κB pathway, thus promoting the MES transition of glioma cells. In addition, Hypoxia Inducible Factor 1 was confirmed to directly bind to the PDIA3P1 promotor region and activate its transcription. In conclusion, PDIA3P1 is a crucial link between hypoxia and glioma MES transition through the PDIA3P1-miR-124-3p-RELA axis, which may serve as a prognostic indicator and potential therapeutic target for glioma treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma

Wang

Gao

et al. 2020

Cell Death Dis

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“…A pseudogene is a non-coding gene that is not producing protein (18). In this study, we identified two fusion genes (CIC-DUX4L8, EIF2AK4-ANXA2P2) (Figure 3) with pseudogene partners (DUX4L8, ANXA2P2).…”

Section: Pseudogenes Are Involved In Formation Of Gene Fusionmentioning

confidence: 85%

Identification of Novel Fusion Transcripts in Undifferentiated Pleomorphic Sarcomas by Transcriptome Sequencing

Zheng

Zhang

Cai

et al. 2019

Cancer Genomics Proteomics

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Background/Aim: Undifferentiated pleomorphic sarcoma (UPS) is an aggressive mesenchymal neoplasm characterized by chromosomal instability. The aim of this study was to identify fusion events involved in UPS. Materials and Methods: Transcriptome sequencing was performed to search for new fusion genes in 19 UPS samples, including two paired recurrent (R) and re-recurrent (RR) samples. Results: A total of 66 fusion genes were detected. Among them, 10 novel fusion genes were further confirmed by reverse transcription polymerase chain reaction (RT-PCR) and Sanger sequencing. Retinoblastoma (RB1) fusions (2 cases) were the most recurrent fusion genes. The gene fusions RB1-RNASEH2B, RB1-FGF14-AS1, and E2F6-FKBP4 were correlated with the Rb/E2F pathway. Pseudogenes were involved in the formation of the gene fusions CIC-DUX4L8 and EIF2AK4-ANXA2P2. Importantly, targetable gene fusions (PDGFRA-MACROD2 and NCOR1-MAP2K1) were detected in UPS. Conclusion: Screening for the presence of fusion transcripts will provide vital clues to the understanding of genetic alterations and the finding of new targeted therapies for UPS.

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“…LncRNAs can alter gene expression through multiple regulatory approaches, such as chromatin modification, transcriptional regulation, and post‐transcriptional regulation (Jalali et al, 2015; Harrap, 2016; Laham‐Karam et al, 2017; Chen et al, 2018). Aberrant expression of lncRNAs has been proved to be associated with abnormal biological behaviors of malignant tumors, such as cervical cancer, hepatocellular carcinoma, glioma, and breast cancer (Gao et al, 2017; Kong et al, 2017; Luan et al, 2017; Aalijahan and Ghorbian, 2019). Besides, lncRNAs can regulate the expression of their nearby genes to regulate cancer progression (Zhu et al, 2016; Su et al, 2017; Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

YY1‐induced upregulation of FOXP4‐AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells

Yang

et al. 2020

Cell Biology International

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Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non‐coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio‐informatics prediction, forkhead box P4 antisense RNA 1 (FOXP4‐AS1) and forkhead box P4 (FOXP4) were upregulated in esophageal carcinoma samples and were positively correlated with each other. The present study aimed to explore the function of FOXP4‐AS1 and FOXP4 in ESCC cells. Function assays disclosed that knockdown of FOXP4‐AS1 or FOXP4 efficiently suppressed cell proliferation and induced cell apoptosis. Moreover, FOXP4‐AS1 positively regulated FOXP4 by interacting with insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) to stabilize FOXP4 messenger RNA. In addition, FOXP4‐AS1 could upregulate the expression of FOXP4 by sponging miR‐3184‐5p. Finally, we found that Yin Yang 1 (YY1) is a transcription factor that can transcriptionally activate both FOXP4‐AS1 and FOXP4 in ESCC cells. In a word, YY1‐induced upregulation of FOXP4‐AS1 and FOXP4 promote the proliferation of ESCC cells

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Pseudogene PDIA3P1 promotes cell proliferation, migration and invasion, and suppresses apoptosis in hepatocellular carcinoma by regulating the p53 pathway

Cited by 59 publications

References 38 publications

Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma

Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma

Identification of Novel Fusion Transcripts in Undifferentiated Pleomorphic Sarcomas by Transcriptome Sequencing

YY1‐induced upregulation of FOXP4‐AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells

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