Pseudomonas atagosis sp. nov., and Pseudomonas akappagea sp. nov., New Soil Bacteria Isolated from Samples on the Volcanic Island Izu Oshima, Tokyo

Abstract

During the exploration of microbial natural resources, two strains of Pseudomonas, PS14 T and PS24 T , were isolated from samples taken from Izu Oshima, a volcanic island located 120 km southwest of central Tokyo. Phylogenetic analysis based on 16S rRNA gene sequences showed that PS14 T was most similar to Pseudomonas baetica a390 T (99.6%) and Pseudomonas helmanticensis OHA11 T (99.5%), and that PS24 T was most similar to Pseudomonas qingdaonensis JJ3 T (98.8%) and Pseudomonas lutea OK2 T (98.7%). The major fatty acids of these two strains were C 16:0 and C 17:0 cyclo, summed feature 3 (C 16:1 ω6c and/or C 16:1 ω7c), and summed feature 8 (C 18:1 ω7c and/or 18:1 ω6c). The phylogenetic analyses, DNA-DNA hybridization results and phenotypic traits indicated that PS14 T and PS24 T constitute two novel species, Pseudomonas atagosis sp. nov. (type strain PS14 T = CECT 9940 T , = LMG 31496 T) and Pseudomonas akappagea sp. nov. (type strain PS24 T = CECT 9941 T , = LMG 31497 T), respectively. The sequence data of the draft genomes of PS14 T and PS24 T were deposited in the Gen-Bank database under accession numbers VXCA00000000 and VXCP00000000, respectively, and the sequence data of their 16S rRNA genes were deposited in the GenBank database under accession numbers MN396717 and MN382268, respectively.

Pseudomonas germanica sp. nov., isolated from Iris germanica rhizomes

“…The resulting supernatant was discarded, and the cell pellet was resuspended in 0.22 µm sterile-filtered cryo-protectant solution (ATCC Reagent-18) containing 0.75 % (w/v) trypticase soy broth, 10 % (w/v) sucrose, and 5 % (w/v) bovine serum albumin fraction V. Samples (200 or 30 mg) of freeze-dried cells were used for polar lipids and cellular fatty acids composition analysis, respectively. Results from FIT28 T were compared with biochemical features and data from phylogenetic-related species which were extracted from the literature [40,41,45,46,48,50,51]. The most abundant fatty acid in FIT28 T is an aliphatic unsaturated chain of 16 carbons (C 16 : 0 ) which represents the 29.6 % of the total cellular fatty acids (Table 4).…”

“…Percentages of cellular fatty acids composition of strain FIT28 T and related species Species 1, FIT28 T ; 2, 'P. crudilactis' UCMA 17988[46]; 3, P. helmanticensis OHA11 T[51]; 4, P. baetica a390 T[41]; 5, P. atagonensis PS14 T[50]; 6, P. koreensis DSM 16610 T[40,42,44]; 7, P. granadensis LMG 27940 T[40,44]; 8, P. iridis P42 T[48]; 9, P. atacamensis LMG 34516 T[38] and 10, P. moraviensis DSM 16007 T[40,45]. Not detected fatty acids are indicated by − and traces by tr.…”

Pseudomonas rustica sp. nov., isolated from bulk tank raw milk at a German dairy farm

“… *Data from MBT-4 T were obtained in this study. Data for other type strains were obtained from references a , [27]; b , [32]; c , [21]; d , [33]; e , [34]; f , [35]; g , [26]; h , [36]. …”

“…koreensis subgroup was extended by the inclusion of P. atagonensis [26], P. neuropathica [27], " P. crudilactis" [21], P.…”

“… *Data from MBT-4 T were obtained in this study. Data for other type strains were obtained from references a , [27]; b , [32]; c , [21]; d , [33]; e , [34]; f , [35]; g , [26]; h , [36]. †Summed features are fatty acids that cannot be resolved reliably from another fatty acid using the chromatographic conditions chosen.…”