Pseudomonas caeni sp. nov., a denitrifying bacterium isolated from the sludge of an anaerobic ammonium-oxidizing bioreactor

Abstract

Two Gram-negative, rod-shaped, motile, aerobic bacterial strains designated HY-14 T and HY-24 were isolated from the sludge of an anaerobic ammonium-oxidizing bioreactor. The strains could not grow with 5 % (w/v) NaCl, did not produce acid from D-glucose or utilize D-glucose, gluconate or citrate as a sole carbon source. Summed feature 3 and C 16 : 0 were the most abundant fatty acids; hydroxyl fatty acids C 12 : 0 3-OH and C 10 : 0 3-OH were also present. Fatty acid C 12 : 0 2-OH was absent. The DNA G+C contents of strains HY-14 T and HY-24 were 50.6±0.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains HY-14 T and HY-24 formed a monophyletic clade within the genus Pseudomonas. The highest sequence similarities were to Pseudomonas pseudoalcaligenes DSM 50188 T (95.9 %). On the basis of phenotypic and phylogenetic properties, strains HY-14 T and HY-24 are proposed as a novel species of the genus Pseudomonas, for which the name Pseudomonas caeni sp. nov. is proposed. The type strain is HY-14 T (5KCTC 22292 T 5CCTCC AB208156 T ).

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

“…2). The G?C content of strain JQ-41 was 65.8 mol%, which fell within the expected range for the genus Pseudomonas (58-70 mol%) [20] and was very similar to reported P. aeruginosa strains' data [22,32]. In conclusion, based on the morphology, physiobiochemical characteristics, and 16S rDNA gene analysis, as well as the G?C content of the genomic DNA, strain JQ-41 was identified as Pseudomonas aeruginosa.…”

Microbial population changes in anaerobic membrane bioreactor treating landfill leachate monitored by single-strand conformation polymorphism analysis of 16S rDNA gene fragments

“…2E), is represented by clone NK2, which belongs to the Pseudomonadaceae family, and it was closely related to Pseudomonas caeni sp. nov. (98% similarity), a denitrifying bacterium isolated from the sludge of an anaerobic ammoniumoxidizing bioreactor (Xiao et al, 2009). The decrease in the bacterial diversity indicates that the bacterial community was reduced due to the increase of the applied OLR.…”

Pseudomonas formosensis sp. nov., a gamma-proteobacteria isolated from food-waste compost in Taiwan

“…T were C 12 : 0 , C 16 : 0 , C 17 : 0 cyclo, C 19 : 0 cyclo v8c, C 16 : 1 v7c/C 16 : 1 v6c and C 18 : 1 v7c/C 18 : 1 v6c which are generally present in members fo the genus Pseudomonas (Xiao et al, 2009;Pascual et al, 2012). Minor fatty acids are C 10 : 0 3-OH, C 12 : 0 3-OH and iso-C 15 : 0 3-OH.…”

“…TR, Trace (less than 1 %); NA, no data available. Data in columns 3 and 6 are from Pascual et al (2012); data in column 7 was obtained from Xiao et al (2009 …”