Pseudomonas putida Strain FStm2 Isolated from Shark Skin: A Potential Source of Bacteriocin

Abstract

Bacteriocin-producing Pseudomonas putida strain FStm2 isolated from shark showed broad range of antibacterial activity against all pathogens tested except Bacillus subtilis ATCC11774, MRSA N32064, Proteus mirabilis ATCC12453, Enterococcus faecalis ATCC14506, Salmonella typhimurium ATCC51312, Salmonella mutan ATCC25175, and Aeromonas hydrophila Wbf314. Of the three growth media tested in this study, TSB was observed to support the bacteriocin activity the most. While the highest bacteriocin activity was observed for media supplemented with 1 % NaCl, there was an observed reduction in bacteriocin activity with increasing salt concentration. Although the least bacteriocin activity was observed for marine broth, addition of increasing amounts of tryptone, glucose, or yeast extract increased bacteriocin activity. This was, however, contrary to the effect observed when MgSO4 and MnSO4 were added as supplements. In the presence of α-amylase, lipase, DNase, and RNase, a positive effect on bacteriocin production was observed. Proteinase K strongly inhibited bacteriocin production. Furthermore, the bacteriocins produced were heat stable within the temperature range of 30-70 °C. Bacteriocin activity also was not affected within a wide pH range of 3-9. Exposure to detergents did not inhibit the activity of the bacteriocin at the concentrations tested. Instead, a positive effect on the relative activity of produced bacteriocin was observed as sodium dodecyl sulfate (SDS), EDTA, and Tween 20 at 1 % concentration all improved bacteriocin activity when the cell-free supernatant was tested against Serratia marcescens ATCC 13880. The bacteriocin was purified by ammonium sulfate precipitation and gel filtration on a Superdex-200 column. SDS-PAGE analysis of the partially purified bacteriocin revealed an apparent molecular weight of ~32 kDa.

Lactococcus Lactis Strains From Intestinal Organ of Black Tips Shark Carcharhinus Limbatus Producing Nisin-Like Bacteriocin Active Against Shrimp and Fish Pathogens (Vibrio Parahaemolyticus and Vibrio Alginolyticus)

“…isolated from shark. These include Lactobacilus pentosus isolated from grey carpet shark Chiloscyllium punctatum (Jiang et al, 2017); a non-LAB bacteria of Halomonadaceae family isolated from a dead shark (Zhen-Xing et al, 2016); Bacillus isolated from deep sea shark (Bindiya et al, 2015); and Pseudomonas putida isolated from shark skin (Ahmad et al, 2013). The objectives of our study was to isolate and study the potential LAB strains from the intestine of a Black tip shark Carcharhinus limbatus, originated from Malaysian East Coast coastal water.…”

Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances

“…Moreover, the inhibitory activity decreased by 90% when treated with a protease, suggesting the presence of protein compounds in the crude extract. These characteristics are commonly related to some bacteriocins produced by Pseudomonas strains [56]. Additional studies are required to confirm the chemical nature of the inhibitory substances isolated in this work.…”

Impact of bacteriocins on multidrug‐resistant bacteria and their application in aquaculture disease prevention and control

“…The stability of the bacteriocin was maintained within a range of 30-70 C and pH 3-9, but the activity was greatly affected by the culture conditions of the strain. 145 In addition to possessing antimicrobial properties, bacteriocins may also play a role in immune regulation. Bacteriocins can act as signalling peptides, regulating the host immune system by signalling through quorum sensing or signalling cells of the host immune system.…”