Pseudomonas sp Isolated from Wastewater and their Interaction with Microalgae

Abstract

During biodegradation processes in Wastewater Treatment Plants (WWTP), bacteria play an important role, and associations of bacteria with microalgae have the potential to remediate wastewater by reducing pollutants. This work aimed to identify bacteria present in wastewater and to analyze their interaction with C. vulgaris to favor their phytoremediation. The physicochemical and microbiological characterization of the wastewater from the Alseseca-Sur WWTP was carried out, identifying the bacterial isolates, a phytoremediation treatment was performed with C. vulgaris and wastewater as a culture medium, quantifying the proliferation of C. vulgaris in the supplemented culture medium and the wastewater. The physicochemical parameters decreased after phytoremediation, the algae-bacteria system caused a decrease in COD due to its application in symbiosis; Pseudomonas sp. prevailed with 75%; C. vulgaris showed optimal growth when wastewater was used as culture medium, inoculating C. vulgaris in wastewater at 100%, 174 cells/mL were quantified compared to 24 cells/mL of C. vulgaris with bold medium plus drinking water. The phytoremediation process in conjunction with the bacterial load effluent from the Alseseca-Sur WWTP showed a decrease in the values of physicochemical parameters; in addition to increasing the number of cells/mL of C. vulgaris using water from the effluent of the Alseseca-Sur WWTP as a culture medium.

A Review on Production of Polyhydroxyalkanoates in Microorganisms

“…Enoyl-CoAhydratase plays a role as a catalyst in the (R)-specific hydration of 2-enoyl-CoA which in turn supplies (R)-3-hydroxyacyl-CoA monomers for the synthesis of PHA by fatty acid β-oxidation pathway (Zhang et al, 2019). P.oleovorans, P. aeruginosa, Burkholderia caryophylli can synthesize mcl-PHAs while, R. eutropha is unable to do so (Pérez-Nava et al, 2021). These organisms accommodate two phaC genes which are split apart by a phaZ gene in the phaC1ZC2D operon.…”

Microalgae-bacteria nexus for environmental remediation and renewable energy resources: Advances, mechanisms and biotechnological applications

“…The type of growth media greatly influences the efficiency of microalgae-bacteria interactions, impacting biomass production and nutrient removal efficiency. Pérez-Nava et al [ 115 ] demonstrated that C. vulgaris and Pseudomonas sp. have enhanced growth in wastewater compared to Bold's basal media, with microalga growth diminishing as the ratio of Bold's basal media increases.…”

Ruta graveolens methanol extract, fungal-mediated biosynthesized silver nanoparticles, and their combinations inhibit pathogenic bacteria

“…The inhibition zones ranged from 7 to 24 mm, and all of the tested bacteria (K. pneumonia, E. coli, E. cloacae, S. aureus, S. epidermidis, Shigella sp., and P. aeruginosa) were susceptible to it to varied degrees (Table 1). These variations in the sensitivity of gram-positive and gram-negative bacteria to nanoparticles may result from these differences in the cell surfaces of these bacteria [22,23]. The activity of the AgNPs (such as antimicrobial efficacy) depends on their specific surface areas.…”