Pseudomonas sp. NGC7 as a microbial chassis for glucose-free muconate production from a variety of lignin-derived aromatics and its application to the production from sugar cane bagasse alkaline extract

Akutsu, Miho; Abe, Nanase; Sakamoto, Chiho; Kurimoto, Yuki; Sugita, Haruka; Tanaka, Makoto; Higuchi, Y; Sakamoto, Kimitoshi; Kamimura, Naofumi; Kurihara, Hiroyuki; Masai, Eiji; Sonoki, Tomonori

doi:10.1016/j.biortech.2022.127479

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…The engineered strains such as Amycolatopsis sp. [80], Corynebacterium glutamicum [81,82], Rhodococcus opacus [83,84], Escherichia coli [74,[85][86][87], and Pseudomonas putida KT2440 [23,[88][89][90][91][92][93][94] have been reported to produce high ccMA titers from lignin-related aromatics. As a general rule, different systems biocatalysis approaches rely on the blockage of ccMA degradation pathways to favor its accumulation and on the overexpression of heterologous enzymes involved in the conversion of lignin-derived aromatics into ccMA.…”

Section: Ciscis-muconic Acidmentioning

confidence: 99%

“…To bypass the constrain for the application of the P. putida KT2440 strain in the ccMA production, and to employ reaction mixtures containing syringyl nucleus compounds as sources for cell growth, the novel Pseudomonas sp. NGC7 strain, isolated as syringateassimilating bacterium [110], was used [93]. The plasmid pTS119 carrying the pcaHG, aroY, catA, and vanAB genes was introduced in a NGC7 mutant strain not expressing the PcaHG and CatB enzymes (NGC703/pTS119).…”

Section: Escherichia Colimentioning

confidence: 99%

“…In an oxygen-dissolved (DO)-stat fed-batch culture and lowering the DO up to 2.5%, ccMA accumulated up to 35.4 mol% yield (15 g/L) from a mixture of vanillic acid and 4-HBA when the initial feeding rate of the substrates (0.2 mL/h) was increased twice and three times 42 h and 50 h after the inoculation, respectively (Table 3). The engineered strain successfully grew on the sugar cane bagasse alkaline extract prepared via a mild alkaline treatment and accumulated ccMA with a 18.7 mol% yield without sugar feeding (Table 3) [93].…”

Section: Escherichia Colimentioning

confidence: 99%

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Lignin Valorization: Production of High Value-Added Compounds by Engineered Microorganisms

et al. 2023

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Lignin is the second most abundant polymer in nature, which is also widely generated during biomass fractionation in lignocellulose biorefineries. At present, most of technical lignin is simply burnt for energy supply although it represents the richest natural source of aromatics, and thus it is a promising feedstock for generation of value-added compounds. Lignin is heterogeneous in composition and recalcitrant to degradation, with this substantially hampering its use. Notably, microbes have evolved particular enzymes and specialized metabolic pathways to degrade this polymer and metabolize its various aromatic components. In recent years, novel pathways have been designed allowing to establish engineered microbial cell factories able to efficiently funnel the lignin degradation products into few metabolic intermediates, representing suitable starting points for the synthesis of a variety of valuable molecules. This review focuses on recent success cases (at the laboratory/pilot scale) based on systems metabolic engineering studies aimed at generating value-added and specialty chemicals, with much emphasis on the production of cis,cis-muconic acid, a building block of recognized industrial value for the synthesis of plastic materials. The upgrade of this global waste stream promises a sustainable product portfolio, which will become an industrial reality when economic issues related to process scale up will be tackled.

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Section: Ciscis-muconic Acidmentioning

confidence: 99%

Section: Escherichia Colimentioning

confidence: 99%

Section: Escherichia Colimentioning

confidence: 99%

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Lignin Valorization: Production of High Value-Added Compounds by Engineered Microorganisms

et al. 2023

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“…Such products have high application value in the manufacture of nylon 66, polytrimethylene terephthalate (PTT), polyethylene terephthalate (PET), industrial plastics, resins, pharmaceuticals, and food ingredients [ [5] , [6] , [7] , [8] , [9] ]. In particular, when 1,4-butadiene, a building block for synthetic rubbers, is produced from ccMA in a ferulate decarboxylase mutant, the global market of ccMA alone is estimated to be over USD 100 million [ 5 , 7 , [10] , [11] , [12] ]. Given its versatility in superior performing bioproducts, ccMA has been identified by Shanks and Keeling as a “bio-privileged” molecule [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas

Wang

et al. 2023

Synthetic and Systems Biotechnology

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“…Species of Pseudomonas bacteria have been identi ed from soils, plants, animals, marine habitats, and clinical materials, making it one of the most varied and pervasive bacterial genera (Clark et al, 2006 Lignin is the most prevalent heterogeneous aromatic polymer found on Earth, the complicated structure presents a signi cant obstacle in biore nery processes since it is not readily susceptible to degradation. Some members of genus Pseudomonas contribute signi cantly in lignin degradation and transformation because they can produce laccase, lignin peroxidase and manganese peroxidase (Akutsu et al, 2022;Nawaz et al, 2023). Nawaz et al isolated Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas oryzisoli sp. nov., isolated from the soil of rice fields

Zang,

Ma,

et al. 2023

Preprint

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A Gram-negative, rod-shaped, and aerobic bacterium Z8T, was isolated from rice fields. It hydrolyses starch and urea, reduces nitrate, and is Tween-20, -40, and − 80, peroxidase and oxidase positive. It grows best at 35°C, 0.0–1.0% NaCl, and pH 7.0. The primary fatty acids found in the membrane are C16:0, C16:1 ω7c and/or C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The predominant quinone system is ubiquinone-9. Strain Z8T is most closely related to Pseudomonas khazarica TBZ2T (98.59%) and Pseudomonas guguanensis JCM 18416T (98.35%) according to similarity and phylogenetic analysis based on 16S rRNA. The genome of strain Z8T comprises 4,468,130 bp, G + C content is 62.95%. The average nucleotide identity with strains of genus Pseudomonas is 77.73–89.05%, dDDH values between Z8T and genus Pseudomonas is 21.90–36.20%. Based on the polyphasic analysis, a novel species with the name Pseudomonas oryzisoli sp. nov. is proposed, and type strain is Z8T (= CCTCC AB2022325T = JCM 35791T).

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Pseudomonas sp. NGC7 as a microbial chassis for glucose-free muconate production from a variety of lignin-derived aromatics and its application to the production from sugar cane bagasse alkaline extract

Cited by 8 publications

References 27 publications

Lignin Valorization: Production of High Value-Added Compounds by Engineered Microorganisms

Lignin Valorization: Production of High Value-Added Compounds by Engineered Microorganisms

Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas

Pseudomonas oryzisoli sp. nov., isolated from the soil of rice fields

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