Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.

Moussous, A.; Paris, Cédric; Khelifi-Slaoui, Majda; Bekhouche, Mohamed; Zaoui, Djamila; Rosloski, Sarah Marie; Makhzoum, Abdullah; Desobry, Stéphane; Khelifi, Lakhdar

doi:10.1007/s11627-017-9862-1

Cited by 16 publications

(8 citation statements)

References 47 publications

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“…The syntheses of the metabolites reach the greatest levels in a certain period of the culture, and metabolite accumulation in cultures harvested before or after this period could signi cantly reduce (Lee et al 2001;Moussous et al 2018;Ono et al 1998). Therefore, in this study, L-Phe applications as a precursor and different culture durations were used to determine their effects on root growth, alkaloid and phenolic production in H. niger adventitious root cultures and to create a protocol that provides high metabolite yield.…”

Section: Discussionmentioning

confidence: 99%

“…). Therefore, it is possible to increase metabolite productivity by determining the most appropriate culture duration when synthesis is at the highest level for in vitro secondary metabolite production (Moussous et al 2018). Secondary metabolite yield will be low if in vitro plants are not harvested during the period when secondary metabolite production is high.…”

Section: Discussionmentioning

confidence: 99%

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L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.

Demırcı

Albayrak

Baydar

2021

Plant Cell Tiss Organ Cult

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This study was aimed to determine the effects of different concentrations of L-phenylalanine (0.25, 0.50 and 1.00 mM L-Phe) applied to adventitious root cultures for different culture durations on root growth and production of tropane alkaloids and phenolics in Hyoscyamus niger. For this purpose, adventitious roots obtained from petiole explants of in vitro seedlings were used as plant materials. After adventitious roots were cultured in liquid MS medium, L-Phe was applied to the adventitious roots. Then the roots were harvested on the 1st, 3rd, and 7th day of the applications, fresh and dry root weights, root growth indexes were analysed. It was also examined the contents of tropane alkaloids (hyoscyamine and scopolamine) and phenolics (gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, p-coumaric, ocoumaric acid, rutin, caffeic acid, ferulic acid, chlorogenic acid, and quercetin) by HPLC in both adventitious roots and culture medium. As a result, the greatest root growth values were obtained from the roots harvested on the 3rd and 7th days after 0.50 and 1.00 mM L-Phe applications. The maximum scopolamine and hyoscyamine amounts were detected in the cultures with the application of 0.50 mM L-Phe for 3 days. In terms of phenolics, 0.50 mM and 1.00 mM L-Phe application with 1st and 3rd days were selected as the most appropriate L-Phe applications and culture duration. Consequently, it has been determined that in vitro secondary metabolite production may be increased in H. niger root cultures with the selection of appropriate L-Phe concentrations and culture durations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.

Demırcı

Albayrak

Baydar

2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

L-Phenylalanine Applications and Culture Duration Affect Root Growth and Production of Tropane Alkaloids and Phenolics in Adventitious Root Cultures of Hyoscyamus Niger L.

Demırcı

Albayrak

Baydar

2021

Preprint

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This study was aimed to determine the effects of different concentrations of L-phenylalanine (0.25, 0.50 and 1.00 mM L-Phe) applied to adventitious root cultures for different culture durations on root growth and production of tropane alkaloids and phenolics in Hyoscyamus niger. For this purpose, adventitious roots obtained from petiole explants of in vitro seedlings were used as plant materials. After adventitious roots were cultured in liquid MS medium, L-Phe was applied to the adventitious roots. Then the roots were harvested on the 1st, 3rd, and 7th day of the applications, fresh and dry root weights, root growth indexes were analysed. It was also examined the contents of tropane alkaloids (hyoscyamine and scopolamine) and phenolics (gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, p-coumaric, o-coumaric acid, rutin, caffeic acid, ferulic acid, chlorogenic acid, and quercetin) by HPLC in both adventitious roots and culture medium. As a result, the greatest root growth values were obtained from the roots harvested on the 3rd and 7th days after 0.50 and 1.00 mM L-Phe applications. The maximum scopolamine and hyoscyamine amounts were detected in the cultures with the application of 0.50 mM L-Phe for 3 days. In terms of phenolics, 0.50 mM and 1.00 mM L-Phe application with 1st and 3rd days were selected as the most appropriate L-Phe applications and culture duration. Consequently, it has been determined that in vitro secondary metabolite production may be increased in H. niger root cultures with the selection of appropriate L-Phe concentrations and culture durations.

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“…In B. candida hairy roots, elicitation with pectinase have also increased the release of scopolamine by 1500% and hyoscyamine by 1100% ( Pitta Alvarez et al., 2003 ). Bacterial strains of Pseudomonus were used as biotic elicitors in D. stramonium hairy roots, which enhanced hyscyamine and scopolamine production maximally by 583% and 265% respectively ( Moussous et al., 2018 ). Chitosan as a biotic elicitor has showed 2.5 to 3-fold enhancement in hyoscyamine production in hairy roots of Hyoscyamus muticus ( Oksman-Caldentey et al., 1991 ).…”

Section: Strategies Adopted For Enhancement Of Secondary Metabolite P...mentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. ‘Hairy roots’, mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the in vitro production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with Agrobacterium rhizogenes. With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.

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Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.

Cited by 16 publications

References 47 publications

L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.

L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.

L-Phenylalanine Applications and Culture Duration Affect Root Growth and Production of Tropane Alkaloids and Phenolics in Adventitious Root Cultures of Hyoscyamus Niger L.

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

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