Pseudomonas spp. ISOLATED FROM THE ORAL CAVITY OF HEALTHCARE WORKERS FROM AN ONCOLOGY HOSPITAL IN MIDWESTERN BRAZIL

Lima, Ana Beatriz Mori; Leão-Vasconcelos, Lara Stefânia Netto de Oliveira; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; André, María Claudia; Barbosa, María Alves; Prado-Palos, Marinésia Aparecida

doi:10.1590/s0036-46652015000600009

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…The role of Bacillus acidophilus as a cariogenic agent was reported earlier this century [32,33]. Early studies reported diverse microorganisms as cariogenic agents like Streptococcus mutans [34][35][36], S. sanguinis, Bacillus cereus [37], L. acidophilus [38], S. aureus [39][40][41][42], S. sobrinus [43,44], Pseudomonas stutzeri [45], S. haemolyticus [46]. L. rhamnosus, and L. salivarus found in the current study, which belongs to the casei group of Lactobacillus [47].…”

Section: Discussionsupporting

confidence: 77%

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

et al. 2022

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Among oral diseases, dental caries is one of the most frequent to affect human health. The current research work aimed to ascertain the antibacterial, anti-biofilm, and antioxidative potential of Piper betle leaf extract against bacteria isolated from dental caries. Analysis for the presence of phytochemical compounds revealed compounds, such as tannins, steroids, phenolic compounds, and alkaloids, which were also confirmed by TLC and FTIR. GC-MS analysis elucidated the presence of 20 phytocompounds, among which were some well-reported bioactive compounds. The chloroform extract of P. betle demonstrated good antibacterial activity (7 mm) and minimum inhibitory concentration (MIC) (100 mg mL−1) against Bacillus gaemokensis MW067143, which was the frequent biofilm producer among isolated bacterial strains. Fractions of the extract were isolated through column chromatography, after which the antibacterial activity was again evaluated. Spirost-8-en-11-one,3-hydroxy(3β,5α,14β,20β,22β,25R), an oxosteroid in nature, was observed to exhibit remarkable antibacterial potential (12 mm) against B. gaemokensis. Bacterial cells treated with P. betle extract had elevated SOD, APOX, POX, and GR activity, while its proteolytic activity against whole bacterial proteins was pronounced with the suppression of several proteins (50, 40, 15, and 10 kDa) in SDS-PAGE. Bacterial cells treated with P. betle extract demonstrated decreased growth, while the extract was also observed to exhibit inhibition of biofilm formation (70.11%) and demolition of established B. gaemokensis biofilms (57.98%). SEM analysis revealed significant changes to bacterial morphology post treatment with P. betle, with cellular disintegration being prominent. In silico network pharmacology analysis elucidated proteins like ESR1 and IL6 to be majorly involved in biological pathways of dental caries, which also interact with the protective ability of P. betle. Gene Ontology (GO) terms and KEGG pathways were also screened using enrichment analysis. Molecular docking demonstrated the highest binding affinity of Spirost-8-en-11-one,3-hydroxy-,(3β,5α,14β,20β,22β,25R) with bacterial proteins FabI (−12 kcal/mol), MurB (−17.1 kcal/mol), and FtsZ (−14.9 kcal/mol). Therefore, it is suggested that P. betle can serve a potentially therapeutic role and could be used in the preparation of herbal formulations for managing bacterial flora.

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Section: Discussionsupporting

confidence: 77%

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

et al. 2022

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“…On the other hand, P. fluorescens , E. coli , and A. haemolyticus also appeared more frequently than other isolated Gram-negative species. P. fluorescens, which is nonpathogenic, is mainly identified at low levels in the microflora of the human body, and it accounts for 8.3% of Gram-negative bacteria found in oral flora [29]. The prevalence of E. coli and A. haemolyticus in the oral cavity was also estimated and identified as associated with dental caries and periodontitis [25].…”

Section: Discussionmentioning

confidence: 99%

Quantitative and qualitative profile of aerobic bacteria in the second mandibular molar free of caries and caries, based on a case–control study

AL-Janabi

2022

Reviews and Research in Medical Microbiology

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Bacteria growing in the oral cavity are an important effective factor in causing caries. Molars are the most commonly affected by bacterial decay. Bacterial species and their counts in caries of the second mandibular molar compared with those in caries-free teeth were investigated. A total of 100 patients with caries in the second mandibular molar and 95 caries-free individuals participated in a case–control study. A swab from the molar zone was cultured to identify the bacterial species. Colony counts and the frequency with which isolated bacteria occur were also determined. The isolated bacteria comprised 34 species ranging between 22 Gram-positive and 12 Gram-negative bacteria from caries molar and 14 species from the control group were diagnosed. Staphylococcus aureus and Kocuria rosea were revealed to have a more significant frequency in caries patients, whereas Kocuria kristinae was isolated with a high frequency from caries-free individuals. The majority of isolates had a large number of colonies, mostly Gram-positive bacteria. Some species have been found in one group of participants but not in another. Six bacterial species were only isolated from control group, whereas the patient group had 16 species that were not found in the control group. In conclusion, Gram-positive bacteria are most frequently associated with caries on the second mandibular molar. Some species of Gram-positive bacteria such as S. aureus and K. rosea and Gram-negative bacteria such as Pseudomonas fluorescens and Escherichia coli occur with great diversity and quantity with caries of the second mandibular molar compared with that of healthy individuals. Meanwhile, some species may disappear or be limited to the decayed area of the second mandibular molar.

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“…Furthermore, Pseudomonas spp. was isolated from the saliva of 15% of all healthcare workers from an oncology hospital in Mid-western Brazil 8. Occupational exposure in healthcare workers also may be considered as a risk factor of bacterial colonisation 9…”

Section: Discussionmentioning

confidence: 99%

Septic pulmonary embolism caused byPseudomonas aeruginosaafter a CO₂laser surgery for rhinitis

Nishizawa

Kanemura²,

Jinta

et al. 2019

BMJ Case Rep

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A 26-year-old healthy patient had a fever and chest pain three days after nasal carbon dioxide (CO2) laser surgery for chronic and allergic rhinitis. In the emergency room, he was diagnosed as a right pneumothorax and managed as outpatients with oral antibiotic therapy and close follow-up. Six days later, in follow-up clinic, his presenting signs and symptoms included right chest pain, tachypnoea and elevated levels of white blood cell count and C reactive protein. He was diagnosed as septic pulmonary embolism (SPE) by the detection of multiple nodules with cavitation on chest CT. Culture of pleural fluids showed Pseudomonas aeruginosa. Intravenous antibiotic treatment and drainage of the pleural effusion improved his condition. Since SPE occurred after nasal CO2 laser surgery in this case, careful attention should be paid to infectious complications of nasal CO2 laser surgery.

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Pseudomonas spp. ISOLATED FROM THE ORAL CAVITY OF HEALTHCARE WORKERS FROM AN ONCOLOGY HOSPITAL IN MIDWESTERN BRAZIL

Cited by 14 publications

References 12 publications

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Quantitative and qualitative profile of aerobic bacteria in the second mandibular molar free of caries and caries, based on a case–control study

Septic pulmonary embolism caused byPseudomonas aeruginosaafter a CO₂laser surgery for rhinitis

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Pseudomonas spp. ISOLATED FROM THE ORAL CAVITY OF HEALTHCARE WORKERS FROM AN ONCOLOGY HOSPITAL IN MIDWESTERN BRAZIL

Cited by 14 publications

References 12 publications

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Quantitative and qualitative profile of aerobic bacteria in the second mandibular molar free of caries and caries, based on a case–control study

Septic pulmonary embolism caused byPseudomonas aeruginosaafter a CO2laser surgery for rhinitis

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Septic pulmonary embolism caused byPseudomonas aeruginosaafter a CO₂laser surgery for rhinitis