PSI-15 Embryo viability based upon energy

Abstract

The objective of this study was to analyze embryo energy release and whether or not it is indicative of survivability upon successful implantation. In both bovine and equine reproduction, fertility is a lowly heritable trait that has an exceedingly large financial impact on the ability to grow genetic lines. In order to increase pregnancy rates, producers must have access to accurate decision-making tools. Nineteen (n = 19) fertilized bovine embryos collected via flushing by licensed veterinarian, Dr. Russell Killingsworth, DVM, in Shamrock, TX, were assessed for energy release using amplification of video with a Nikon Camera and Iolight Microscope. Once embryos were flushed, they were filmed for a thirty-five (35) second observation period. Embryo dimensions were then taken using Image J software. Embryo release of energy was humanly perceptible and measurable by changes in their morphological dimensions. Measurements of significance were taken on the embryo intercellular mass area (ICM), total embryo area, and on the vertical, diagonal, and horizontal axis of the sub-zonal distance, or the distance between the zona pellucida and outer edge of the ICM. All measurements were taken in microns. Once embryos were successfully measured, all were transferred into recipients. Significant findings presented that embryos with a greater ICM, total embryo area, and sub-zonal vertical, diagonal, and horizontal distance established pregnancies more frequently than embryos with less momentous differences in distance (P < 0.05). This research has shown the relationship between embryo energy and pregnancy had a significance of determining pregnancy viability. This has therefore resulted in enhanced prediction of embryo transfer techniques based on embryo quality making pregnancy rates for producers more predictable and consistent.