PSII Fluorescence Techniques for Measurement of Drought and High Temperature Stress Signal in Crop Plants: Protocols and Applications

Brestič, Marián; Živčák, Marek

doi:10.1007/978-81-322-0807-5_4

Cited by 208 publications

(179 citation statements)

References 196 publications

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“…Strasser et al (2000) introduced the performance index (PI abs ) as the product of three independent parameters combining structural and functional criteria: density of reaction centers, the quantum efficiency of primary photochemistry and conversion of excitation energy in electron transport. This parameter was found to be much more sensitive to environmental effects than commonly used parameter F v /F m (Brestič and Živčák 2013) and it was shown to be well correlated with photosynthetic capacity measured as CO 2 assimilation (van Heerden et al 2003, Ripley et al 2004. Later, the total performance index (PI tot ) was introduced (Smit et al 2009).…”

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confidence: 98%

Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency

Živčák

Olšovská

Slamka

et al. 2014

Plant Soil Environ.

120

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Nitrogen deficiency influences importantly the plant photosynthetic capacity and crop productivity. Here, we employed the rapid, non-invasive measurements of chlorophyll a fluorescence kinetics for calculation of the integrative fluorescence parameters related to the leaf photosynthetic performance. In pot experiments with winter wheat (Triticum aestivum L.) we cultivated plants during the whole growing period in the soil substrate supplied with four different doses of nitrogen. The leaf nitrogen and chlorophyll content as well as the plant dry mass were analyzed after chlorophyll fluorescence records in three growth stages. Our results indicate that the commonly used parameter F v /F m (the maximum quantum yield of photochemistry) was almost insensitive to nitrogen treatment. In contrary, the performance index (PI abs ) and total performance index (PI tot ) were much more responsive and significant differences among plants of different nitrogen treatments as well as between the youngest and third leaf from the top were observed. Parameter PI tot was shown to express only small diurnal changes, thus being more reliable and more useful for comparison of different samples in field conditions than more frequently used parameter PI abs .

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mentioning

confidence: 98%

Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency

Živčák

Olšovská

Slamka

et al. 2014

Plant Soil Environ.

120

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“…Several authors observed the decrease in PI values with the advancing drought in different crops (Strasser et al 2000;Živčák et al 2008). In adult trees this reaction is probably the manifestation of acute stress and primary photochemical processes are more sensitive to high temperatures than to drought (Brestič & Živčák 2013). The gradual increase of the values during the growing season is the result of the annual dynamics of pigment content, and hence, it is the natural change in the efficiency of photochemical processes.…”

Section: Chlorophyll Fluorescence Measurementsmentioning

confidence: 99%

Photosynthetic response of European beech to atmospheric and soil drought

Priwitzer¹,

Kurjak²,

Kmeť³

et al. 2014

Forestry Journal

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Physiological response of European beech under soil and atmospheric drought conditions was investigated in this study. A group of six beech trees was irrigated during the growing season 2012, while the second group of non-irrigated (control) beech trees was treated under natural soil drought. During the experiment, we observed more than 45-day long period when no precipitation fell on the soil surface. The relationship of P N (CO 2 assimilation rate) to g S (stomatal conductance) was very tight in both groups, which indicates that stomatal opening was the main factor limiting P N . The statistically significant differences in g S between the groups of trees were revealed only on the last measuring day. The significant differences in P N were confirmed on the days when the differences in soil water potential (Ψ S ) appeared. On these measurement days, the P N values of irrigated individuals were approximately 1.9 or 3.3 times greater than the values of non-irrigated individuals. At the level of primary photosynthetic processes (chlorophyll fluorescence parameters) we did not observe lower values of the control individuals in comparison with the irrigated trees in any of the evaluated parameters. Long-term soil water deficit caused strong decrease of leaf water potential (Ψ L ) in the control trees, but Ψ L values of the irrigated trees were also rather low due to diurnal dynamics in higher parts of crown. Close relationship between Ψ L and g S was confirmed for the control (non-irrigated) trees, but could not be confirmed for the irrigated trees. We revealed significant influence of VPD (vapour pressure deficit of the air) on g S only in the control group. On the days when Ψ S decreased, the stomata of the non-irrigated trees were closed in spite of the low VPD values. Almost complete stomatal closure in both groups of trees was caused by the increase of VPD to 1.2 kPa. Keywords: Fagus sylvatica; water deficit; vapour pressure deficit; leaf water potential; stomatal closure Abstrakt Príspevok sa zaoberá skúmaním fyziologických reakcií buka na atmosférické a pôdne sucho. Skupina šiestich bukov bola zavlažovaná počas vegetačnej sezóny 2012, pričom druhá skupina bukov (kontrola) bola ponechaná v prirodzených pôdnych vlahových podmienkach. Počas experimentu bola pozorovaná viac než 45 dní trvajúca perióda, kedy sa zrážková voda nedostala na povrch pôdy. Potvrdili sme silnú závislosť rýchlosti asimilácie (P N ) na prieduchovej vodivosti (g S ), čo dokazuje, že otvorenosť prieduchov bola hlavným limitujúcim faktorom priebehu asimilácie CO 2 . Štatisticky významné rozdiely g S medzi skupinami boli potvrdené len počas posledného merania. Významné rozdiely v P N sa potvrdili pre dni, v ktorých sme zistili výrazné rozdiely vo vodnom potenciáli pôdy (Ψ S ). Počas týchto dní boli priemerné hodnoty P N pre zalievané stromy 1,9, resp. 3,3-krát vyššie v porovnaní s nezavlažovanými jedincami. Na úrovni primárnych fotosyntetických procesov (parametre fluorescencie chlorofylu) sme nezistili nižšie hodnoty kontrolných jedin...

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“…Additionally, specific automated imaging systems can analyze chlorophyll fluorescence emitted by the green parts of plants (Chen et al, 2014). Unlike to majority of imaging methods, the parameters of chlorophyll fluorescence are directly associated with physiological processes, mostly with photosynthesis (Kalaji et al, 2016) with broad possibilities to detect effects of stress factors (Brestic and Zivcak, 2013;Zivcak et al, 2014;Brestic et al, 2015, in press). Using the special technical application of chlorophyll fluorescence may detect also some qualitative traits associated with content of biologically active phenolic compounds (Sytar et al, , 2016.…”

Section: Integration Of Imaging Techniques In High Throughput Phenotymentioning

confidence: 99%

Applying hyperspectral imaging to explore natural plant diversity towards improving salt stress tolerance

Sytar

Brestič

Živčák

et al. 2017

Science of The Total Environment

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• Salinity represents an abiotic stress constraint affecting growth and productivity of plants • Better solutions is to improve the level of salt resistance using natural genetic variability within crop species • Phenomic methodology employing different non-invasive imaging systems for detecting quantitative and qualitative changes caused by salt stress at the whole plant and canopy level. Hyperspectral imaging techniques provide unique opportunities for fast and reliable evaluation of numerous characteristics associated both with various structural, biochemical and physiological traits • Salt-soil-plant interaction and sustainable coastal agriculture need powerful phenotyping tools Salinity represents an abiotic stress constraint affecting growth and productivity of plants in many regions of the world. One of the possible solutions is to improve the level of salt resistance using natural genetic variability within crop species. In the context of recent knowledge on salt stress effects and mechanisms of salt tolerance, this review present useful phenomic approach employing different non-invasive imaging systems for detection of quantitative and qualitative changes caused by salt stress at the plant and canopy level. The focus is put on hyperspectral imaging technique, which provides unique opportunities for fast and reliable estimate of numerous characteristics associated both with various structural, biochemical Science of the Total Environment 578 (2017) 90-99 ⁎ Corresponding authors at:

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PSII Fluorescence Techniques for Measurement of Drought and High Temperature Stress Signal in Crop Plants: Protocols and Applications

Cited by 208 publications

References 196 publications

Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency

Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency

Photosynthetic response of European beech to atmospheric and soil drought

Applying hyperspectral imaging to explore natural plant diversity towards improving salt stress tolerance

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