2019
DOI: 10.1016/j.scijus.2018.08.005
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Psychedelic fungus (Psilocybe sp.) authentication in a case of illegal drug traffic: sporological, molecular analysis and identification of the psychoactive substance

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Cited by 14 publications
(10 citation statements)
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“…The HRM melting curve has prominent capacity to discriminate DNA barcodes based on their composition, length, or GC content, which allows for the examination of genetic variations within DNA sequences. Moreover, it supports forensic evidence of the suspicious species [19][20][21][22][23]. For example, it has been demonstrated that HRM could identify Cannabis sativa and "legal high" drugs, among other species [21].…”
Section: Introductionsupporting
confidence: 62%
See 1 more Smart Citation
“…The HRM melting curve has prominent capacity to discriminate DNA barcodes based on their composition, length, or GC content, which allows for the examination of genetic variations within DNA sequences. Moreover, it supports forensic evidence of the suspicious species [19][20][21][22][23]. For example, it has been demonstrated that HRM could identify Cannabis sativa and "legal high" drugs, among other species [21].…”
Section: Introductionsupporting
confidence: 62%
“…The identification of fungi at the species level is critical for many research areas, such as health sciences, agriculture, and forensic science, and for determining whether hallucinogenic mushrooms are essential for treatment or are being trafficked as drugs [13,16,22]. In addition, studies have shown that psilocybin may relieve depression and so this HRM method could also be utilized for research in this area [30].…”
Section: Discussionmentioning
confidence: 99%
“…The reaction mixture for real‐time PCR and HRM analysis consisted of a total volume of 15 µl. This contained 7.5 µl of 2× Kapa SYBR qPCR Mix (Kapa Biosystems Roche), (Cowan & Elkins, ; Solano et al, ) 0.2 µM forward primer, 0.2 µM reverse primer, and 1 µl of 25 ng/µl DNA. SYBR fluorescence dye was used to monitor the accumulation of the amplified product during PCR and the HRM process to derive the T m value.…”
Section: Methodsmentioning
confidence: 99%
“…HRM analysis is the method of choice for rapid analysis of sequence variation within PCR amplicons by determining their melting temperature (T m ). 22 T m depends on the base composition of amplicons. T m differences can be detected by monitoring the fluorescence changes during PCR cycles using real-time PCR differential analysis.…”
Section: Introductionmentioning
confidence: 99%