PTD-J domain acts as an antigen carrier for cell mediated immunity

Abstract

BALB/c mice elicited specific cell-mediated immunity after transplantation with CT26 cells stably expressing human telomerase reverse transcriptase (CT26(hTERT#10)). Restingmemory T cells were reactivated in vitro by CT26(hTERT#10) cells and PTD-J-hTERTepi recombinant proteins, in which the polypeptide hTERTepi comprised TH and TC epitopes, as indicated by interferon γ (IFNγ) secretion. Preand post-injections of PTD-J-hTERTepi with in vitro expanded and differentiated dendritic cells reduced CT26(hTERT#10) tumor size. Moreover, Hsp72 activated numerous splenocytes to perform IFNγ secretion and further reduced tumor sizes.

PTD-J-X: To Elicit Humoral and Cellular Immunity as Will

“…Furthermore, mice pre-treated or post-treated with PTD-J-hTERTepi could diminish the transplanted CT26 (hTERT#10) tumor more quickly than the untreated control. Hsp72 could enhance this effect significantly in the post-treatment cases [6]. It is curious how could Hsp72 augment both humoral and cellular immunity?…”