Pterostilbene administration improves the recovery potential of extremely low-frequency magnetic field in acute renal ischemia-reperfusion injury: An FTIR spectroscopic study

Abstract

Renal ischemia-reperfusion (I/R) injury, one of the drastic outcomes of renal failure and organ transplantation, tends to deteriorate over time; therefore, noninvasive therapeutic strategies will avail the progression-free survival of the patients. Magnetic field has been proposed as a noninvasive treatment strategy; however, with recent scientific advances, many controversies have arisen regarding its efficacy. Pterostilbene, a natural analog of resveratrol, was documented to be effective in treatment of I/R injuries. This study aims to assess the acute therapeutic effects of combined extremely low-frequency magnetic field (ELF-MF) and pterostilbene treatment on renal I/R injury. After induction of renal I/R in Wistar rats, treatments of 50 Hz, 1 mT ELF-MF applied alone or in combination with pterostilbene were applied for 5 consecutive days. Kidney homogenates were analyzed by Fourier transform infrared spectroscopy. I/R injury resulted in an altered protein and lipid structure with the dominance of longer acyl chains; a slight decrease in lipid, protein, unsaturated lipid, and unsaturated/saturated lipid content; and an increase in membrane fluidity and lipid peroxidation in rat kidneys. Although ELF-MF treatment alone was not sufficient to restore all ischemia-induced alterations, the combined treatment strategy of pterostilbene administration in the presence of ELF-MF was successful and warrants further investigation.

Doksorubisinin Karaciğer Dokusunda Oluşturduğu Hasarın ve Propolisin Bu Hasar Üzerindeki Potansiyel Koruyucu Etkisinin Değerlendirilmesi

“…Changes in the parameters associated with the order and fluidity of the phospholipids, the main components of the cellular membrane, affect the normal functioning of membranes. The shifts towards higher values detected in the wavenumbers of the CH2 antisymmetric and symmetric stretching bands after DOX administration indicated a decrease in the order and an increase in the acyl chain flexibility of membrane lipids (11,16). A significant increase observed in the bandwidth of the CH2 antisymmetric stretching band indicated that DOX caused an increase in the fluidity of membrane.…”

“…CH2 antisym./CH3 antisym. ratio was utilized to detect the changes in the chain length of phospholipids, while the C=O/lipid and CH3 antisym./lipid ratios were utilized to detect the changes in the carbonyl status of and methyl concentration in the tissue, respectively (11,16). As seen from Table 2, the CH2 antisym./CH3 antisym.…”

“…To obtain information about the structural alterations in the proteins, the ratio of Amide I/Amide II was calculated (11) and it has been seen that this ratio decreased significantly in the DOX and DOX + PRPLS treated groups compared to control group. In addition, to compare the relative alterations in the amounts of lipids and proteins, the lipid/protein ratio was calculated (16). This ratio increased significantly in the DOX and DOX + PRPLS treated groups in comparison with control group (Table 2).…”

“…The alterations in the wavenumbers of the CH2 antisymmetric and symmetric stretching bands and bandwidth of the CH2 antisymmetric stretching band were analyzed to examine the order and fluidity of the biological membrane (16). As seen from Table 3, the wavenumber values of the CH2 antisymmetric and symmetric stretching bands shifted to higher values in the DOX and DOX + PRPLS treated groups and the bandwidth of the CH2 antisymmetric stretching band increased significantly in the same groups compared to control group.…”