Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Siedlecka-Kroplewska, Kamila; Jóźwik, Agnieszka; Kaszubowska, Lucyna; Kowalczyk, Anna; Bogusławski, Wojciech

doi:10.5603/fhc.2012.0080

Cited by 19 publications

(7 citation statements)

References 19 publications

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“…In our study, we showed that pterostilbene has dose-dependent cytotoxic effects on Jurkat and Hut-78 cells after 24 and 48 h treatment. This effect has also been observed in acute myeloid leukemia and the MOLT-4 human lymphoblastic leukemia cell line [21, 22]. At the same time, we found that the doses of pterostilbene we used in our present study are safe.…”

Section: Discussionsupporting

confidence: 86%

Pterostilbene Induces Cell Apoptosis and Cell Cycle Arrest in T-Cell Leukemia/Lymphoma by Suppressing the ERK1/2 Pathway

Chang

Xiao

et al. 2017

BioMed Research International

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Pterostilbene is a natural 3,5-dimethoxy analog of trans-resveratrol that has been reported to have antitumor, antioxidant, and anti-inflammatory effects. T-cell leukemia/lymphoma is one of the more aggressive yet uncommon non-Hodgkin lymphomas. Although there has been increasing research into T-cell leukemia/lymphoma, the molecular mechanisms of the antitumor effects of pterostilbene against this malignancy are still largely unknown. The aim of this study is to confirm the effects of pterostilbene in T-cell leukemia/lymphoma. Jurkat and Hut-78 cells treated with pterostilbene were evaluated for cell proliferation using Cell Counting Kit-8, and apoptosis, cell cycle progression, reactive oxygen species generation, and mitochondrial membrane potential were analyzed using flow cytometry. The level of protein expression was detected by western blot. The results demonstrated that pterostilbene significantly inhibited the growth of T-cell leukemia/lymphoma cell lines in vitro and induced apoptosis in a dose- and time-dependent manner. Moreover, pterostilbene treatment markedly induced S-phase cell cycle arrest, which was accompanied by downregulation of cdc25A, cyclin A2, and CDK2. Pterostilbene also induced the generation of reactive oxygen species and the loss of mitochondrial membrane potential and inhibited ERK1/2 phosphorylation. Taken together, our study demonstrated the potential of pterostilbene to be an effective treatment for T-cell leukemia/lymphoma.

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Section: Discussionsupporting

confidence: 86%

Pterostilbene Induces Cell Apoptosis and Cell Cycle Arrest in T-Cell Leukemia/Lymphoma by Suppressing the ERK1/2 Pathway

Chang

Xiao

et al. 2017

BioMed Research International

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“…It has been shown that dose-dependent PTER induces apoptosis in HL60 cell at concentrations similar to those used in our investigation [32,33,42,43]. Furthermore, our research confirmed that, PTER induces apoptosis in MOLT 4 [43], MV4-11, U937, and THP-1 human leukemia cell lines [32].…”

Section: Discussionsupporting

confidence: 87%

“…Antitumor activity of stilbene derivatives has been shown in vitro in many cell lines. The induction of apoptosis in cancer cells by stilbene derivatives is well-documented [17,26,32,33,34,35,36,37,38,39,40,41,42,43]. Stilbene derivatives have been shown to have antitumor activity due to several mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Imaging Flow Cytometric Analysis of Stilbene-Dependent Apoptosis in Drug Resistant Human Leukemic Cell Lines

et al. 2019

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Background: The natural compounds have been researched extensively as an alternative to the conventional chemotherapy and radiation. Stilbene derivatives appear as a group of therapeutics which deserves special attention. The present study was designed to analyze the effects of stilbene derivatives on drug resistant human leukemic cells. The aim of this work was to evaluate the apoptotic effect of stilbene derivatives in various concentrations on leukemic cells (LC) with and without resistant phenotype. Methods: Human acute promyelocytic leukemia (APL) cell lines (HL60, HL60/MX1, HL60/MX2) and acute lymphoblastic leukemia (ALL) cell lines (CEM/C1, CCRF-CEM) were studied. T-resveratrol, piceatannol, rhaponticin, deoxyrhaponticin, pterostilbene were used to stimulate apoptosis. Mitoxantrone (MIT) was applied to induce drug resistance. Results: t-Resveratrol (RES), deoxyrhaponticin (D-RHAP), rhaponticin (RHAP), pterostilbene (PTER), and piceatannol (PIC) influenced viability and induced apoptosis in all investigated cell lines. Conclusions: Our results confirmed that RES, PIC, RHAP, D-RHAP, and PTER are essential therapeutic compounds with anticancer activity exhibited by induction of apoptosis in leukemic cells with and without resistant phenotype. Stilbene-induced apoptosis in HL60/MX1, HL60/MX2, CEM/C1, and CCRF-CEM leukemia cell lines have been presented in very few studies so far and our research is an important contribution to the investigation of these substances.

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“…48,49 Also, PTS has been shown to cause a transient accumulation of cells in the G 0 /G 1 -cell cycle phase followed by the S-phase arrest in MOLT4 human leukemia cells. 50 Almost the same observations were reported by 10 PTS-induced apoptosis due to ROS generation in MCF7 and nitric oxide over production in PC3 cells. It was concluded previously that PTS exerted its anticancer action in breast cancer by arresting cells in G0/G1, G2/M or S phase.…”

Section: Cell Cycle Progression Analysissupporting

confidence: 65%

Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites

Kutbi¹,

Kammoun²,

El-Telbany³

2021

IJN

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Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Cited by 19 publications

References 19 publications

Pterostilbene Induces Cell Apoptosis and Cell Cycle Arrest in T-Cell Leukemia/Lymphoma by Suppressing the ERK1/2 Pathway

Pterostilbene Induces Cell Apoptosis and Cell Cycle Arrest in T-Cell Leukemia/Lymphoma by Suppressing the ERK1/2 Pathway

Imaging Flow Cytometric Analysis of Stilbene-Dependent Apoptosis in Drug Resistant Human Leukemic Cell Lines

Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites

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