PTHG2 Reduces Bone Loss in Ovariectomized Mice by Directing Bone Marrow Mesenchymal Stem Cell Fate

Chen, Jiao; Zhang, Hao; Wu, Ximei; Wang, Fuxiao; Wang, Yili; Gao, Qianmin; Liu, Han; Yan, Hu; Su, Jiacan; Jing, Yingying

doi:10.1155/2021/8546739

Cited by 11 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The light microscopes (Olympus BX53 and Nikon ECLIPSE Ci-L plus) were used for section imaging. The number of positive cells or area in sections was quantified using ImageJ ( 56 ).…”

Section: Micro-ct Analysesmentioning

confidence: 99%

Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

et al. 2023

Self Cite

View full text Add to dashboard Cite

Abnormal subchondral bone remodeling featured by overactivated osteoclastogenesis leads to articular cartilage degeneration and osteoarthritis (OA) progression, but the mechanism is unclear. We used lymphocyte cytosolic protein 1 ( Lcp1 ) knockout mice to suppress subchondral osteoclasts in a mice OA model with anterior cruciate ligament transection (ACLT), and Lcp1 −/− mice showed decreased bone remodeling in subchondral bone and retarded cartilage degeneration. For mechanisms, the activated osteoclasts in subchondral bone induced type-H vessels and elevated oxygen concentration, which ubiquitylated hypoxia-inducible factor 1 alpha subunit (HIF-1α) in chondrocytes and led to cartilage degeneration. Lcp1 knockout impeded angiogenesis, which maintained hypoxia environment in joints and delayed the OA progression. Stabilization of HIF-1α delayed cartilage degeneration, and knockdown of Hif1a abolished the protective effects of Lcp1 knockout. Last, we showed that Oroxylin A, an Lcp1- encoded protein l -plastin (LPL) inhibitor, could alleviate OA progression. In conclusion, maintaining hypoxic environment is an attractive strategy for OA treatment.

show abstract

“…The light microscopes (Olympus BX53 and Nikon ECLIPSE Ci-L plus) were used for section imaging. The number of positive cells or area in sections was quantified using ImageJ ( 56 ).…”

Section: Micro-ct Analysesmentioning

confidence: 99%

Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Caplan coined the term MSCs in 1991 [ 6 ]. One of the defining features of MSCs is the tri-lineage differentiation capacity including osteogenesis, chondrogenesis and adipogenesis [ [7] , [8] , [9] ]. MSCs were considered to be an appropriate cell source for bone tissue engineering [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Wang¹,

Guo²,

Wang³

et al. 2022

Journal of Orthopaedic Translation

Self Cite

View full text Add to dashboard Cite

“…cAMP is involved in the regulation of preadipocyte differentiation ( Yadav and Jang, 2021 ). cAMP signaling is associated with adipogenic differentiation of bone marrow mesenchymal stem cells ( Chen et al, 2021 ). Therefore, we speculate that ADCY2 gene may be involved in adipocyte differentiation and thus affect the formation of gallstones.…”

Section: Discussionmentioning

confidence: 99%

Association Study Between Polymorphic Loci in Cholesterol Metabolism Pathway and Gallstone in the Tibetan Population

Chen

Zhang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Background: The incidence of gallstones in the Tibetan population is increasing rapidly. Previous studies indicated that genetic variation located in the cholesterol metabolism pathway may be associated with the incidence of gallstones.Methods: By recruiting 132 Tibetan gallstone patients and 52 normal Tibetan controls, we performed next-generation sequencing for 508 genes in the cholesterol metabolism pathway. Additionally, by integrating the sequence data of 41 normal Tibetan subjects in the public database, we finally obtained 93 normal Tibetan controls. Single nucleotide polymorphisms (SNPs) calling were performed by using the GATK pipeline. The quality control criteria for SNPs were: missing rate <0.05; minor allele frequency (MAF) > 0.01; and p value >0.001 in the Hardy-Weinberg Equilibrium (HWE) test. To eliminate the influence of population heterogeneity, Principal Component Analysis (PCA) was carried out by using the smartpca software. Association analyses were performed by Plink software. Multiple tests were adjusted by the false discovery rate (FDR) method.Results: A total of 2,401 SNPs were obtained by analyzing 508 genes, and 2,011 SNPs left after quality control. After adjusting the eigen vectors, we found that 10 SNPs (SNV05997, rs80145081, rs80005560, rs79074685, rs748546375, rs201880593, rs142559357, rs750769471, rs869789 and rs4072341) were significantly associated with gallstone. Subsequently, by comparing the case group with our control group and the public database control group separately, we further found that the SNP rs869789 was consistently significantly associated with gallstone (p = 9.04 × 10–3 in cases vs. our controls and 5.73 × 10–3 in cases vs. public controls, respectively).Conclusion: By systematically analyzed SNPs in the cholesterol metabolism pathway, we identified one polymorphic locus rs869789 significantly associated with the pathogenesis of gallstone in the Tibetan population. This study will provide clue for further mechanism study of gallstone in the Tibetan population.

show abstract

PTHG2 Reduces Bone Loss in Ovariectomized Mice by Directing Bone Marrow Mesenchymal Stem Cell Fate

Cited by 11 publications

References 60 publications

Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Association Study Between Polymorphic Loci in Cholesterol Metabolism Pathway and Gallstone in the Tibetan Population

Contact Info

Product

Resources

About