2003
DOI: 10.1182/blood-2003-04-1308
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PTP-MEG2 is activated in polycythemia vera erythroid progenitor cells and is required for growth and expansion of erythroid cells

Abstract: Polycythemia vera (PV) is a human clonal hematologic disorder. Previously we demonstrated that erythroid colony-forming cells (ECFCs) from PV patients contained a hyperactive membrane-associated tyrosine phosphatase. We now show that this phosphatase corresponded to protein tyrosine phosphatase (

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Cited by 42 publications
(41 citation statements)
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“…Plasmids and Retrovirus Production-Retroviral MSCV-IRES-GFP (pMIG) plasmid expressing human PTPN9 and its substrate trapping mutant D470A (DA) cDNAs were as described (17). pCMV plasmid expressing N-terminal FLAGtagged PTPN9 WT and PTPN9 DA were generated by PCR.…”
Section: Methodsmentioning
confidence: 99%
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“…Plasmids and Retrovirus Production-Retroviral MSCV-IRES-GFP (pMIG) plasmid expressing human PTPN9 and its substrate trapping mutant D470A (DA) cDNAs were as described (17). pCMV plasmid expressing N-terminal FLAGtagged PTPN9 WT and PTPN9 DA were generated by PCR.…”
Section: Methodsmentioning
confidence: 99%
“…Antibodies, Immunoblotting, and Immunoprecipitation-Anti-PTPN9 rabbit serum was described previously (17 , and EGFR antibodies were from Cell Signaling Technology, Inc. Anti-STAT3, STAT5, ErbB2, Shc, Akt, and ERK1/2 antibodies were purchased from Santa Cruz Biotechnology, Inc. Anti-phosphotyrosine antibody (4G10) was from Millipore. For Western blot, cells were directly lysed in 1ϫ SDS sample buffer.…”
Section: Methodsmentioning
confidence: 99%
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“…Interestingly, PTPN9 has been shown to play an important role in the development of erythroid cells. 35 To test that PTPN9 is a miR-126 target, a standard luciferase assay was set up in which a fragment of the 3Ј-UTR of PTPN9 that contains the seed region was inserted downstream of a luciferase open reading frame. Constructs containing a mutated or deleted sequence of the seed region were produced as controls.…”
Section: Ptpn9 Is a Target Of Mir-126mentioning
confidence: 99%
“…Initial studies targeted the cytokine receptors and their ligands, and later the individual signaling proteins and transcription factors were examined. [11][12][13][14][15][16][17][18][19] The discovery of gainof-function mutations in the Janus kinase 2 (JAK2) by different experimental approaches has been a foreseeable outcome of these efforts. [20][21][22][23][24] The presence of the valine 617 to phenylalanine mutation of JAK2 (JAK2-V617F) has been detected across the MPN entities and at low frequency in other clonal myeloid disorders.…”
Section: Introductionmentioning
confidence: 99%