pUGTdb: A comprehensive database of plant UDP-dependent glycosyltransferases

Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4′-di-O-glycosyltransferase from Ziziphus jujuba var. spinosa

“…Glycosylation is an important post-modification biosynthetic reaction in plant secondary metabolism 1 . This type of reaction is generally catalyzed by uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) 2 . Thus far, a huge family of glycosyltransferases (GTs) have been discovered from plants 3 .…”

“… 1 This type of reaction is generally catalyzed by uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs). 2 Thus far, a huge family of glycosyltransferases (GTs) has been discovered from plants. 3 They provide powerful tools to prepare important bioactive glycosides, including schaftoside, 4 stevioside, 5 glycyrrhizin, 6 and ginsenosides.…”

Discovery, mechanisms, and engineering of the missing apiosylation step in the biosynthesis of flavonoid apiosides in Leguminosae plants

“…The aglycones could be flavanones (1,(3)(4)(5)(6), chalcones (2, 7-8), flavones (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22), isoflavones (23)(24)(25)(26)(27), flavonols (28)(29)(30)(31), and dihydrochalcone (32) To fully identify structures of the products, we purified six 2-O-apiosides (6a, 15a, 24a, 27a, 32a, and 35a) from scaled-up catalytic reactions. All the products are new compounds except for 15a 21 .…”

“…The UGTmediated glycosylation reactions have only one step, and show high catalytic efficiency and selectivity. Thus far, a big family of plant UGTs have been reported, and most of them accept popular sugar donors such as UDP-glucose (UDP-Glc), UDP-xylose (UDP-Xyl), UDP-galactose (UDP-Gal), UDP-arabinose (UDP-Ara), and UDPglucuronic acid (UDP-GlcA) 13,14 . It is noteworthy that no reported UGTs could accept UDP-apiose (UDP-Api) as the sugar donor.…”

Functional characterization of two efficient glycosyltransferases catalysing the formation of rutin from Sophora japonica L.

“…6 This reaction is generally catalysed by uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs). 7 The biosynthesis of rutin may involve two UGTs, a flavonoid 3- O -glucosyltransferase and a 6′′- O -rhamnosyltransferase. Thus far, dozens of flavonoid 3- O -glycosyltransferases have been identified from plants, including NpUGT6 8 from Nelumbo nucifera , SmUF3GT 9 from Salvia miltiorrhiza , CsUGT72AM1 10 from Camellia sinensis , Sb3GT1 11 from Scutellaria baicalensis , VvGT1 12 and VvGT6 13 from Vitis vinifera , UGT78K1 14 from Glycine max , and AtUGT73B3 15 and AtUGT78D2 15 from Arabidopsis thaliana .…”