Pullulans produced by strains of Cryphonectria parasitica—I. Production and characterisation of the exopolysaccharides

Forabosco, Andrea; Bruno, Giovanni; Sparapano, Lorenzo; Liut, Gianfranco; Marino, Deborah R.; Delben, F.

doi:10.1016/j.carbpol.2005.10.005

Cited by 62 publications

(24 citation statements)

References 28 publications

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“…Roukas and Biliaderis (1995) initially precipitated crude polysaccharide with two volumes of acetone, dissolved in distilled water and reprecipitated with absolute ethanol. Pullulan precipitation by addition of ethanol at 4°C has been reported by a number of workers (Chi & Zhao, 2003;Forabosco et al, 2006;Göksungur, Dagbagli, Uçan, & Güvenç, 2005). Kachhawa et al (2003) compared different solvents including methanol, ethanol, isopropyl alcohol, ethyl methyl ketone, tetrahydrofuran and acetone for efficiency of pullulan recovery and reported ethanol to be most efficient.…”

Section: Screening Of Organic Solventsmentioning

confidence: 95%

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Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Singh

Saini

Kennedy³

2009

Carbohydrate Polymers

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Section: Screening Of Organic Solventsmentioning

confidence: 95%

“…Pullulan precipitation using two volumes of ethanol at 4°C for 1 h has been reported (Göksungur et al, 2005;Lazaridou et al, 2002). Forabosco et al (2006) treated culture filtrates with three volumes of cold ethanol for pullulan precipitation at À20°C for 24 h.…”

Section: Time Of Precipitationmentioning

confidence: 99%

Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Singh

Saini

Kennedy³

2009

Carbohydrate Polymers

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“…Besides A. pullulans, Rhodotorula bacarum has also been reported to produce a large amount of melanin-free pullulan [2]. Recently, Forabosco et al [3] reported that pullulan is also produced by Cryphonectria parasitica, which is a fungal agent of chestnut blight. Some distinctive properties of pullulan, such as structure flexibility, enhanced water-solubility, and excellent film-and fiber-forming properties, are attributed to regular alternation of α-(1→4) and α-(1→6) linkages in the ratio of 2:1 [4].…”

mentioning

confidence: 99%

Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

Singh

Saini

2008

Appl Biochem Biotechnol

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Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.

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“…Polysaccharides from plant extract attract interesting in drug industry due to their unique biological, chemical and physical properties [10,36]. These naturally occurring compounds were highly recommended because they have less toxicity and have much fewer undesirable side effects than the drugs currently in use [33].…”

Section: Introductionmentioning

confidence: 99%

Effect of Longan polysaccharides on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro

Zhu

Zhou

Liu

et al. 2015

Med Biol Eng Comput

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For autologous chondrocyte implantation (ACI) to restore cartilage defect, limited cell numbers and dedifferentiation of chondrocytes are the major difficulties. An alternative is the use of growth factors, but the high cost and potential tumorigenesis are the major obstacles. To ensure successful ACI therapy, it is of significance to find effective substituted pro-chondrogenic agent. Polysaccharides from plant extract have low toxicity and few undesirable side effects, which were reported to facilitate cartilage regeneration. In this study, we investigated the effect of Longan polysaccharides (LP) on rabbit articular chondrocytes through examination of the cell proliferation, morphology, viability, glycosaminoglycan synthesis and cartilage-specific gene expression. Results showed that close to the positive group which used the growth factor of TGF-β, LP could effectively promote chondrocytes growth and enhance secretion and synthesis of cartilage extracellular matrix by up-regulating expression levels of aggrecan, collagen II and sox9 compared to the negative control. Expression of collagen I gene was effectively down-regulated, demonstrating the inhibition of chondrocytes dedifferentiation by LP. Hypertrophy that might lead to chondrocyte ossification was also undetectable in LP groups. Range of 4.69-18.76 µg/ml was recommended dose of LP, among which the most profound response was observed with 9.38 μg/ml. All the evidences revealed that LP may replace the growth factors to be applied in ACI therapy. This study might provide a basis for development of a novel agent in the treatment of articular cartilage defect.

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Pullulans produced by strains of Cryphonectria parasitica—I. Production and characterisation of the exopolysaccharides

Cited by 62 publications

References 28 publications

Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

Effect of Longan polysaccharides on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro

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