Pulmonary edema measured by MRI correlates with late-phase response to allergen challenge

Evans, Rhys L.; Changani, K. Kumar; Hotee, Sarah; Pindoria, Kashmira; Campbell, Scott E.; Nials, Anthony T.; Ford, William R.; Broadley, Kenneth J.; Kidd, Emma Jane

doi:10.3109/01902148.2014.985407

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…5C), which showed no differences in mRNA expression between WT and Chi3l1 À/À mice. Though more commonly measured in acute lung injury, pulmonary edema can also contribute to AHR in animal models (34) and is present in very severe clinical cases of asthma (35). We analyzed wet/dry ratios of WT and Chi3l1 À/À mice and observed decreased ratios in the latter during fungal asthma.…”

Section: Chitinase 3-like-1/brp-39 Deficiency Does Not Affect Productmentioning

confidence: 99%

Chitinase 3-like-1 protects airway function despite promoting type 2 inflammation during fungal-associated allergic airway inflammation

Mackel

Garth

Jones

et al. 2021

American Journal of Physiology-Lung Cellular and Molecular Phys

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Exposure to fungi can result in a wide range of comorbidities depending on the immune status of the host. Chronic exposure and reactivity to fungi such as Aspergillus fumigatus can result in conditions such as severe asthma with fungal sensitization (SAFS) or allergic bronchopulmonary aspergillosis (ABPA). However, the pathophysiology of SAFS and ABPA are not well understood. Here, we report that the chitinase-like protein YKL-40 is elevated in lung lavage fluid from human asthmatics that are sensitized to fungi. Initial studies demonstrated that mice deficient in the murine ortholog of YKL-40, breast regression protein-39 (BRP-39, chitinase-3-like 1, Chi3l1), were not more susceptible to acute infection with A. fumigatus. However, in an experimental model of fungal-associated allergic airway inflammation (fungal asthma), Chi3l1-/- mice had significantly increased airway hyperresponsiveness (AHR). Surprisingly, increased AHR in Chi3l1-/- mice occurred in the presence of significantly lower type 2 responses (decreased eosinophil numbers and decreased IL-4, IL-5, IL-33, CCL17 and CCL22 levels), although type 1 and type 17 responses were not different. Increased AHR was not associated with differences in Periodic-acid-Schiff staining of lung tissue, differences in the expression of Muc5ac and Clca3, nor differences in lung edema. Bone marrow chimera studies revealed that the presence of BRP-39 in either the hematopoietic or non-hematopoietic compartment was sufficient for controlling AHR during fungal asthma. Collectively, these results indicate that BRP-39 protects against AHR during fungal asthma despite contributing to type 2 inflammation, thus highlighting an unexpected protective role for BRP-39 in allergic fungal asthma.

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Section: Chitinase 3-like-1/brp-39 Deficiency Does Not Affect Productmentioning

confidence: 99%

Chitinase 3-like-1 protects airway function despite promoting type 2 inflammation during fungal-associated allergic airway inflammation

Mackel

Garth

Jones

et al. 2021

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…MRI was increasingly applied also in patients with asthma, demonstrating the occurrence of segmental lung edema and being a robust method for monitoring the response to treatment. [164][165][166][167]. Furthermore, it has been shown that hyperpolarized gases allow the evaluation of functional changes in distal small airways of asthmatic patients.…”

Section: Magnetic Resonance Imaging (Mri) and Pet/mrimentioning

confidence: 99%

Molecular Imaging of Pulmonary Inflammation and Infection

Giraudo

Evangelista

Fraia

et al. 2020

IJMS

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Infectious and inflammatory pulmonary diseases are a leading cause of morbidity and mortality worldwide. Although infrequently used in this setting, molecular imaging may significantly contribute to their diagnosis using techniques like single photon emission tomography (SPET), positron emission tomography (PET) with computed tomography (CT) or magnetic resonance imaging (MRI) with the support of specific or unspecific radiopharmaceutical agents. 18F-Fluorodeoxyglucose (18F-FDG), mostly applied in oncological imaging, can also detect cells actively involved in infectious and inflammatory conditions, even if with a low specificity. SPET with nonspecific (e.g., 67Gallium-citrate (67Ga citrate)) and specific tracers (e.g., white blood cells radiolabeled with 111Indium-oxine (111In) or 99mTechnetium (99mTc)) showed interesting results for many inflammatory lung diseases. However, 67Ga citrate is unfavorable by a radioprotection point of view while radiolabeled white blood cells scan implies complex laboratory settings and labeling procedures. Radiolabeled antibiotics (e.g., ciprofloxacin) have been recently tested, although they seem to be quite unspecific and cause antibiotic resistance. New radiolabeled agents like antimicrobic peptides, binding to bacterial cell membranes, seem very promising. Thus, the aim of this narrative review is to provide a comprehensive overview about techniques, including PET/MRI, and tracers that can guide the clinicians in the appropriate diagnostic pathway of infectious and inflammatory pulmonary diseases.

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Pulmonary edema measured by MRI correlates with late-phase response to allergen challenge

Abstract: We show a temporal relationship between edema and the LAR and their parallel reduction, along with eosinophils and AHR, by dexamethasone. This suggests a close causative association between pulmonary edema and impaired airways function.

Cited by 2 publications

References 24 publications

Chitinase 3-like-1 protects airway function despite promoting type 2 inflammation during fungal-associated allergic airway inflammation

Chitinase 3-like-1 protects airway function despite promoting type 2 inflammation during fungal-associated allergic airway inflammation

Molecular Imaging of Pulmonary Inflammation and Infection

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