Pulmonary Involvement in the Antisynthetase Syndrome: A Comparative Cross-sectional Study

Andersson, Helena; Aaløkken, Trond Mogens; Günther, Anne; Mynarek, Georg; Garen, Torhild; Lund, May Brit; Molberg, Øyvind

doi:10.3899/jrheum.151067

Cited by 25 publications

(18 citation statements)

References 41 publications

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“…To study the interactions, 50 mg matrices (S-R and S-Y) were soaked with 1 mL of bLf solutions (0.063-10 mg/mL) in adsorption buffer (20 mM sodium phosphate buffer, pH 7.0) for 16 h at 25ЊC. The concentration of commercial bLf in the supernatants was determined spectrophotometrically at 280 nm considering an absorption coefficient of 1.51 mL/mg·cm [20]. The equilibrium concentration of bLf bound to the matrix per unit of total amount of matrix was calculated by difference between the concentration of bLf at the beginning of the experiment and that remaining in the soluble phase after adsorption.…”

Section: Methodsmentioning

confidence: 99%

Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand

et al. 2014

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The worldwide production of whey increases by around 186 million tons each year and it is generally considered as a waste, even when several whey proteins have important economic relevance. For its valorization, inexpensive ligands and integrated chromatography methods need to be developed for specific and low-cost protein purification. Here, we describe a novel affinity process with the dye Yellow HE-4R immobilized on Sepharose for bovine lactoferrin purification. This approach based on a low-cost ligand showed an efficient performance for the recovery and purification of bovine lactoferrin directly from whey, with a yield of 71% and a purification factor of 61.

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Section: Methodsmentioning

confidence: 99%

Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand

et al. 2014

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“…Altogether, 54 of the 68 patients (79%) had been diagnosed with myositis by their treating physician prior to study inclusion; 27 with PM, 25 with DM and two with hypomyopathic/amyopathic DM. For comparison, 97% were diagnosed with ILD [23]. The 54 ASS patients with a prior myositis diagnosis had longer median disease duration (76 months, range 6–232) than the 14 cases without myositis (22 months, range 7–230; p < 0.001).…”

Section: Resultsmentioning

confidence: 99%

“…The study cohort has been described elsewhere [23]. Briefly, the cohort included 68 ASS patients and 67 healthy age and sex-matched controls examined at Oslo University Hospital between September 2011 and June 2014.…”

Section: Methodsmentioning

confidence: 99%

Comparative analyses of muscle MRI and muscular function in anti-synthetase syndrome patients and matched controls: a cross-sectional study

et al. 2017

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BackgroundMagnetic resonance imaging (MRI) of thigh muscles is increasingly used to assess disease activity and damage extent in chronic myositis, but the validity of the findings is not clear. Here, the primary aim was to compare thigh MRI findings in patients having chronic myositis associated with anti-synthetase syndrome (ASS) and in matched healthy controls.MethodsCross-sectional analyses of thigh muscle MRI, muscular function and creatinine kinase (CK) were performed in 68 ASS patients (median disease duration 71 months) and 67 controls matched for age and gender. MRI changes associated with disease activity (edema in muscles and fascia) and damage (fatty replacement and muscle volume reduction) were assessed semiquantitatively, giving a total MRI score of 0–78 (total edema 0–42 and total damage 0–36).ResultsASS patients had higher total MRI score than the matched controls (14.1 versus 3.0; p < 0.001) and less muscle strength (p < 0.001). Muscle edema was more frequent in ASS patients than controls (38% versus 12%), as was fatty replacement (42% versus 4%). In ASS patients, we found that the total edema score correlated with CK, but 23% of the patients with normal CK had score > 18. Muscle compartment analyses in ASS patients showed that muscle edema was most pronounced anteriorly, while fatty replacement dominated posteriorly.ConclusionsThis study showed, for the first time, the magnitude of difference in muscle MRI findings between chronic myositis cases and matched controls. In ASS patients, muscle MRI appeared to provide useful complementary information to muscle strength and CK levels in the assessment of myositis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13075-017-1219-y) contains supplementary material, which is available to authorized users.

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“…The AN ligand density was calculated based on the acid volume consumed in the titration. In this study, six AN SepFF adsorbents with ligand densities of 15,20,26,29,55, and 64 mmol/L (herein referred to as AN15 SepFF, AN20 SepFF, AN26 SepFF, AN29 SepFF, AN55 SepFF, and AN64 SepFF) were prepared for the purification of IgG.…”

Section: Preparation Of MMC Adsorbentsmentioning

confidence: 99%

“…In single-column chromatography, however, it is difficult to simultaneously achieve high purity as well as high binding capacity and yield. Accumulating evidence suggests that high binding capacity for organic acids, sugars, or other low-molecular-weight compounds along with high purity and yield can easily be obtained by simulated moving bed (SMB) chromatography, even in cases in which the adsorbents exhibit significant binding of impurities [28][29][30]. Likely, SMB chromatography is widely used in the separation of proteins, plasmids, and even virus [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Improved purification of immunoglobulin G from plasma by mixed‐mode chromatography

Chai

Sun

Wang

et al. 2014

J of Separation Science

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Efficient loading of immunoglobulin G in mixed-mode chromatography is often a serious bottleneck in the chromatographic purification of immunoglobulin G. In this work, a mixed-mode ligand, 4-(1H-imidazol-1-yl) aniline, was coupled to Sepharose Fast Flow to fabricate AN SepFF adsorbents with ligand densities of 15-64 mmol/L, and the chromatographic performances of these adsorbents were thoroughly investigated to identify a feasible approach to improve immunoglobulin G purification. The results indicate that a critical ligand density exists for immunoglobulin G on the AN SepFF adsorbents. Above the critical ligand density, the adsorbents showed superior selectivity to immunoglobulin G at high salt concentrations, and also exhibited much higher dynamic binding capacities. For immunoglobulin G purification, both the yield and binding capacity increased with adsorbent ligand density along with a decrease in purity. It is difficult to improve the binding capacity, purity, and yield of immunoglobulin G simultaneously in AN SepFF chromatography. By using tandem AN SepFF chromatography, a threefold increase in binding capacity as well as high purity and yield of immunoglobulin G were achieved. Therefore, the tandem chromatography demonstrates that AN SepFF adsorbent is a practical and feasible alternative to MEP HyperCel adsorbents for immunoglobulin G purification.

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Pulmonary Involvement in the Antisynthetase Syndrome: A Comparative Cross-sectional Study

Cited by 25 publications

References 41 publications

Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand

Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand

Comparative analyses of muscle MRI and muscular function in anti-synthetase syndrome patients and matched controls: a cross-sectional study

Improved purification of immunoglobulin G from plasma by mixed‐mode chromatography

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